TAXONOMY
Talaromyces haitouensis L. Wang, sp. nov. — Fungal Names FN570868; (Fig. 2)
Etymology: — haitouensis (Lat.): named after the locale where the ex-type strain was isolated.
Diagnosis: T. haitouensis is characterized by normal growth at 25°C and 37°C, green-yellow gymnothecia, orange-brown mycelium, and echinulate broadly ellipsoidal ascospores.
Type: China: Jiangsu: Lianyungang, Haitou Town, Haizhouwan Resort, 34° 55' 57"N, 119° 11' 56"E, 3 m, HMAS 350335, dried culture of ex-type AS3.16101 on MEA, the Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; ex type culture AS3.16101 T= HR1-7 from the riverside soil, 5 May 2021, X-Y. Liu; GenBank: ITS = MZ045695, BenA = MZ054634, CaM = MZ054637, Rpb2 = MZ054631.
Description: On CYA at 25°C after 7 d: Colonies 22–25 mm diam, thin, radially plicate slightly, margins submerged, fimbriate; velutinous and granular due to gymnothecia; gymnothecia limited, Pinard Yellow (R. Pl. IV); sporulation absent or sparse; mycelium Mars Yellow (R. Pl. III) mingled with Orange Vinaceous (R. Pl. XXVII); no exudate and soluble pigment; reverse Morocco Red (R. Pl. I), while Strawberry Pink (R. Pl. I) at margins. On MEA at 25°C after 7 d: Colonies 48–51 mm diam, thin, plane, margins submerged, regular; granular due to gymnothecia; gymnothecia abundant, Green Yellow (R. Pl. V); sporulation absent or limited, Olive-Gray (R. Pl. LI); mycelium Green Yellow (R. Pl. V) while white at margins; no exudate, no soluble pigment; reverse Light Danube Green (R. Pl. XXXII) centrally while Cinnamon Buff (R. Pl. XXIX) at peripheries. On YES at 25°C after 7 d: Colonies 25–28 mm diam, thin, radially sulcate, margins on agar surface, fimbriate; velutinous and granular due to limited gymnothecia at centres; gymnothecia Pinard Yellow (R. Pl. IV); sporulation absent or sparse, Light Olive Gray (R. Pl. LI); mycelium Pecan Brown to Cacao Brown (R. Pl. XXVIII), while white at margins; no exudate and soluble pigment; reverse Morocco Red (R. Pl. I), while Moral Red (R. Pl. I) at margins. On OA at 25°C after 7 d: Colonies 33–35 mm diam, thin, plane, mostly submerged and sparsely granular due to gymnothecia; gymnothecia limited in centres, Light Greenish Yellow (R. Pl. IV); sporulation absent; no exudate and soluble pigment; reverse Sulphur Yellow (R. Pl. V). On Cz at 25°C after 7 d: Colonies 14–17 mm diam, slightly thick, umbonate centrally, plane, margins submerged, fimbriate; velutinous and granular due to gymnothecia; gymnothecia limited, Pinard Yellow (R. Pl. IV); sporulation absent; mycelium Orange-Vinaceous to Pale Vinaceous (R. Pl. XXVII), while white at margins; exudate limited, Strawberry Pink (R. Pl. I); soluble pigment absent or limited, light red; reverse Nopal Red (R. Pl. I). On CYA at 37°C after 7 d: Colonies 18–20 mm diam, slightly thick, irregularly plicate slightly, margins on agar surface, regular; sporulation absent; mycelium Shell Pink (R. Pl. XXVIII); no exudates and soluble pigment; reverse Ochraceous-Salmon (R. Pl. XV). On CYA at 5°C after 7 d: No growth.
Gymnothecia globose, (400–) 450–480 µm, asci globose, 12.5–13 µm, and elliposoidal, 15 × 12–13 µm, maturing after 14 d; ascospores broadly ellipsoidal, 5–6 × 4 µm, echinulate. Conidiophores from aerial hyphae, 20–60 × 2–2.5 µm, smooth-walled, penicilli biverticillate, metulae 2–4, 10–13 × 2–2.5 µm, phialides 2–4, 9–12 × 2–2.5 µm, conidia pyriform to ellipsoidal, 2.5–3 × 2–2.5 smooth-walled.
Talaromyces zhenhaiensis L. Wang sp. nov. — Fungal Names FN570869; (Fig. 3)
Etymology: —zhenhaiensis (Lat.): named after the locale where the ex-type strain was isolated.
Diagnosis: T. zhenhaiensis is characterized by fast growth at 25°C and 37°C, naphthalene-yellow gymnothecia and mycelium, and smooth-walled ellipsoidal ascospores with one equatorial ridge.
Type: China: Zhejiang: Ningbo, Zhenhai, 29°58'19″N 121°48'19"E, 8 m, HMAS 350336, dried culture of ex-type AS3.16102 on MEA, the Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; ex type culture AS3.16102T = ZH3-18 from mudflat soil, 10 Sep 2019, F-H Song; GenBank: ITS = MZ045697, BenA = MZ054636, CaM = MZ054639, Rpb2 = MZ054633.
Description: On CYA at 25°C after 7 d: Colonies 65–67 mm diam, plane, thin, margins submerged, fimbriate, umbonate centrally; velutinous, overlaid with sparse floccose mycelium; sporulation absent; mycelium Sulphur Yellow (R. Pl. V) to Cartridge Buff (R. Pl. XXX), while white at margins; no exudate ; no soluble pigment; reverse Honey Yellow (R. Pl. XXX). On MEA at 25°C after 7 d: Colonies 43–45 mm diam, thin, plane, margins on agar surface, fimbriate; velutinous; sporulation absent, mycelium pale yellow, near Massicot Yellow (R. Pl. IV), while white at margins; immature gymnothecia abundant, white to Massicot Yellow (R. Pl. IV); no exudate, no soluble pigment; reverse Pale Orange Yelllow (R. Pl. XXX). On YES at 25°C after 7 d: Colonies 61–62 mm diam, thin, irregularly sulcate, margins on agar surface, fimbriate; velutinous with sparse floccose mycelium overlaid; no sporulation; mycelium Cartridge Buff (R. Pl. XXX) to Naphthalene Yellow (R. Pl. XVI), white at margins; no exudate and soluble pigment; reverse Raw Sienna (R. Pl. III). On OA at 25°C after 7 d: Colonies 37–40 mm diam, thin, plane, margins on agar surface, regular; granular due to abundant gymnothecia, cream yellow to Naphthalene Yellow (R. Pl. XVI); no sporulation; mycelium white; no exudate and soluble pigment; reverse Sulphur Yellow (R. Pl. V). On Cz at 25°C after 7 d: Colonies 38–40 mm diam, plane, thin, margins submerged, fimbriate; velutinous; sporulation absent; mycelium pale yellow, near Martius Yellow (R. Pl. IV) to Marguerite Yellow (R. Pl. XXX), while white at margins; immature gymnothecia abundant, white to Marguerite Yellow (R. Pl. XXX); no exudate and soluble pigment; reverse Aniline Yellow (R. Pl. IV). On CYA at 37°C after 7 d: Colonies 56–60 mm diam, thin, plane, margins on agar surface, regular; velutinous; no sporulation; mycelium white; no exudate and soluble pigment; reverse Augus Brown (R. Pl. III). On CYA at 5°C after 7 d: No growth.
Gymnothecia globose, 300–350 µm, asci globose to subglobose, 8–10 µm, borne in short chains, maturing after 14 d; ascospores flattened ellipsoidal, 4–5 × 3 µm, smooth-walled, with one equatorial ridge.
Additional strains examined. China: Hainan, Ledong, Mount Jianfengling, 18°42'36″N 108°49'48"E, 800 m; culture AS3.15693 = NN072335 from soil, 8 Nov 2015, X-Z. Jiang; GenBank: ITS = KY007094, BenA = KY007110, CaM = KY007102, Rpb2 = KY112592.