Table 1. Comparison of the physiological and biochemical differences between strain J12C1-MA-4T and phylogenetically related type strains in the family Rhodobacteraceae based on 16S rRNA gene and genome sequences.
1, Gymnodinialimonas ceratoperidinii J12C1-MA-4T; 2, Jannaschia helgolandensis KCTC 12191T; 3, Pseudooctadecabacter jejudonensis KCTC 32525T; 4, Loktanella acticola KCTC 52837T; 5, Loktanella ponticola KCTC 42133T; 6, Alterinioella nitratireducens KCTC 72738T; ND, not determined.
Characteristics
|
1
|
2a
|
3b
|
4c
|
5d
|
6e
|
Isolation source
|
Ceratoperidinium margalefii
|
North Sea water
|
Zone where the ocean and a freshwater spring meet
|
Seawater
|
Seawater
|
Seawater
|
Colony morphology
|
Circular, yellow, smooth
|
small,
whitish, shiny
|
circular, slightly convex, smooth, glistening, reddish orange
|
circular, slightly convex, glistening, smooth, greyish yellow
|
circular, slightly convex, smooth, glistening, greyish-yellow
|
circular, convex, smooth, and nontransparent
|
Growth condition
|
|
|
|
|
|
|
temperature range (oC) (optimum)
|
4–37 (30)
|
15–30 (25–30)
|
15–35 (30)
|
4–30 (25)
|
4–30 (25)
|
10–37 (30)
|
Motile
|
Non-motile
|
Non-motile
|
Non-motile
|
Non-motile
|
Non-motile
|
Non-motile
|
Cell size (μm)
|
1.0–1.8 X
0.2–0.5
|
1.9–3.2 X 0.7–1.1
|
0.2–0.5 X 0.8–5.0
|
0.4–1.2 X >0.4
|
0.2–0.7 X 0.4–7.0
|
0.8–1.2 X 1.0–2.5
|
pH range (optimum)
|
5.0–9.0 (7.0)
|
7.0–8.0 (ND)
|
6.0–8.0 (7.0–8.0)
|
6.0–8.0(7.0–8.0)
|
5.5–8.0(7.0–8.0)
|
6.5–9.5 (8.0)
|
NaCl concentration range (%) (optimum)
|
0–8 (1)
|
1–7 (ND)
|
1–6 (2)
|
0–8.0 (2.0-3.0)
|
0.5–6.0 (2)
|
1.0–11 (3.0)
|
a Data from Wagner et al. (Wagner-Dobler et al. 2003); b Data from Billerbeck et al. (Billerbeck et al. 2015) ; c Data from Park et al. (Park et al. 2017); d Data from Jung et al. (Jung et al. 2014); e Data from Kong et al. (Kong et al. 2021).
Table 2. Comparison of the genome features differences between strain J12C1-MA-4T and phylogenetically related type strains in the family Rhodobacteraceae based on 16S rRNA gene and genome sequences.
1, Gymnodinialimonas ceratoperidinii J12C1-MA-4T; 2, Jannaschia helgolandensis KCTC 12191T; 3, Pseudooctadecabacter jejudonensis KCTC 32525T; 4, Loktanella acticola KCTC 52837T; 5, Loktanella ponticola KCTC 42133T; 6, Alterinioella_nitratireducens KCTC 72738T; ND, not determined.
|
1
|
2a
|
3b
|
4c
|
5d
|
6e
|
ANI
|
-
|
71.59%
|
70.69%
|
-
|
69.58%
|
73.54%
|
AAI
|
-
|
60.55%
|
61.28%
|
-
|
60.18%
|
64.99%
|
dDDH
|
-
|
18.8%
|
20.9%
|
-
|
19.3%
|
18.7%
|
G+C
|
64.48 mol%
|
-
|
-
|
57.3 mol%
|
55.9 mol%
|
65.4 mol%
|
a Data from Wagner et al. (Wagner-Dobler et al. 2003); b Data from Billerbeck et al. (Billerbeck et al. 2015) ; c Data from Park et al. (Park et al. 2017); d Data from Jung et al. (Jung et al. 2014); e Data from Kong et al. (Kong et al. 2021).
Table 3. Biochemical differences between strain J12C1-MA-4T phylogenetically related type starins in the family Rhodobacteraceae based on 16S rRNA gene and genome sequences.
1, Gymnodinialimonas ceratoperidinii J12C1-MA-4T; 2, Jannaschia helgolandensis KCTC 12191T; 3, Pseudooctadecabacter jejudonensis KCTC 32525T; 4, Loktanella acticola KCTC 52837T; 5, Loktanella ponticola KCTC 42133T; 6, Alterinioella_nitratireducens KCTC 72738T; All data were obtained from parallel experiments in this study under certain defined conditions. +, positive; -, negative; w, weak.
Characteristics
|
1
|
2
|
3
|
4
|
5
|
6
|
API 20NE
|
|
|
|
|
|
|
Indoleproduction (TRyptoPhane)
|
-
|
+
|
-
|
-
|
-
|
-
|
Arginine DiHydrolase
|
-
|
+
|
-
|
-
|
+
|
-
|
UREase
|
+
|
+
|
+
|
+
|
-
|
-
|
Acid production (API 50CH)
|
|
|
|
|
|
|
Glycerol
|
-
|
-
|
-
|
+
|
-
|
+
|
L-arabinose
|
W
|
W
|
-
|
-
|
-
|
-
|
L-xylose
|
-
|
W
|
-
|
-
|
-
|
-
|
D-fructose
|
-
|
W
|
-
|
-
|
-
|
-
|
D-mannose
|
-
|
-
|
-
|
-
|
+
|
-
|
D-mannitol
|
-
|
-
|
-
|
+
|
-
|
-
|
Arbutin
|
-
|
-
|
-
|
+
|
-
|
-
|
Esculin ferric citrate
|
+
|
+
|
+
|
+
|
+
|
+
|
D-turanose
|
-
|
-
|
-
|
+
|
-
|
-
|
D-fucose
|
W
|
W
|
-
|
-
|
-
|
-
|
L-fucose
|
-
|
W
|
-
|
-
|
-
|
-
|
Potassium 5-KetoGluconate
|
W
|
W
|
-
|
-
|
-
|
-
|
ZYM
|
|
|
|
|
|
|
Alkaline phosphatase
|
-
|
+
|
+
|
-
|
+
|
+
|
Esterase(C8)
|
+
|
+
|
+
|
-
|
-
|
+
|
Leucine arylamidase
|
+
|
+
|
+
|
+
|
-
|
+
|
Valine arylamidase
|
W
|
W
|
-
|
-
|
-
|
-
|
Cystine arylamidase
|
W
|
-
|
-
|
-
|
-
|
-
|
Acid phosphatase
|
+
|
+
|
+
|
-
|
-
|
-
|
Naphthol-AS-BI-phosphohydrolase
|
W
|
W
|
+
|
+
|
+
|
+
|
α-Galactosidase
|
-
|
W
|
-
|
-
|
-
|
-
|
β-Galactosidase
|
-
|
+
|
-
|
-
|
-
|
-
|
α-glucosidase
|
-
|
-
|
-
|
+
|
-
|
-
|
β-glucosidase
|
-
|
-
|
-
|
+
|
-
|
-
|
Table 4. Cellular fatty acid compositions (>1%) of the strain J12C1-MA-4T and phylogenetically related type strains in the family Rhodobacteraceae based on 16S rRNA gene and genome sequences.
1, Gymnodinialimonas ceratoperidinii J12C1-MA-4T; 2, Jannaschia helgolandensis KCTC 12191T 3, Pseudooctadecabacter jejudonensis KCTC 32525T; 4, Loktanella acticola KCTC 52837T; 5, Loktanella ponticola KCTC 42133T; 6, Alterinioella_nitratireducens KCTC 72738T; All data are from the present study. Values are presented as the percentages of total fatty acids. ND, not detected. Major components (> 10%) are shown in bold.
Fatty acids
|
1
|
2
|
3
|
4
|
5
|
6
|
Saturated
|
|
|
|
|
|
|
C16:0
|
4.11
|
0.69
|
3.73
|
7.71
|
4.79
|
19.94
|
C18:0
|
1.2
|
12.73
|
3.86
|
1.69
|
0.36
|
1.29
|
Hydroxy
|
|
|
|
|
|
|
C10:0 3OH
|
1.98
|
2.95
|
1.77
|
1.63
|
0.99
|
2.14
|
C12:0 3OH
|
ND
|
ND
|
ND
|
ND
|
ND
|
4.31
|
C12:1 3OH
|
ND
|
ND
|
4.51
|
3.27
|
3.16
|
ND
|
C16:0 2OH
|
3.52
|
ND
|
ND
|
ND
|
ND
|
3.07
|
C18:1 2OH
|
3.69
|
ND
|
ND
|
ND
|
ND
|
ND
|
Unsaturated
|
|
|
|
|
|
|
C20:2 ω6,9c
|
ND
|
1.53
|
ND
|
ND
|
ND
|
0.88
|
C18:1 ω7c 11-methyl
|
3.83
|
7.05
|
10.72
|
11.24
|
2.42
|
1.24
|
C19:0 cyclo ω8c
|
ND
|
27.90
|
ND
|
0.33
|
ND
|
31.58
|
Summed feature*
|
|
|
|
|
|
|
2
|
0.17
|
1.58
|
ND
|
ND
|
ND
|
ND
|
3
|
1.05
|
0.38
|
1.17
|
1.53
|
13.56
|
1.47
|
7
|
ND
|
1.76
|
1.74
|
0.75
|
0.33
|
ND
|
8
|
79.14
|
40.66
|
71.37
|
69.85
|
73.61
|
32.17
|
*There are two or three fatty acids divided into a group which cannot be separated by GLC with the MIDI System, stipulated as summed features. Summed feature 2 contains C12:0 aldehyde, unknown 10.928; Summed feature 3 contains C16:1 ω7c and/or C16:1 ω6c, C16:1 ω6c and/or C16:1 ω7c; Summed feature 7 contains C19 : 1ω6cand/or C19 : 1ω7cand/or cyclo C19 : 0. Summed feature 8 contains C18:1 ω7c and/or C18:1 ω6c.