Ethics approval and consent to participate – This is a retrospective data review study without direct involvement of human subjects and as such informed consent was waived by the University of Michigan Institutional Review Board (ID#: HUM00080561 and HUM00085888) to obtain surplus tissues from Department of Pathology archives for study. All methods were carried out in accordance with relevant guidelines and regulations from the University of Michigan ethics committee. All experimental protocols were approved by University of Michigan ethics committee.
Consent for Publication – Not applicable.
Availability of Data - All data generated or analyzed during this study are included in this published article and its supplementary information files as well as deposited online (SRA PRJNA668767).
Competing Interests - The authors declare that they have no competing interests
Funding - M.E.H received funding from NIH grant T32 DC005356. J.C.B. received funding from NIH Grants U01-DE025184, P30-CA046592 and R01-CA194536. M.L. was funded by F31-CA206341-01A1. Funding sources had no role in in the design of the study and collection, analysis, and interpretation of data or in writing the manuscript.
Author Contributions – MEH performed data collection, survival analysis, sequencing analysis, data visualization, siRNA knockout experiments, drafted manuscript, edited manuscript, and approved final version, ACB analyzed sequencing data, drafted manuscript, edited manuscript, and approved final version, ADB performed bioinformatics analysis and contributed to data visualization, edited manuscript, and approved final version, JZ performed formal statistical analysis, edited manuscript, and approved final version, AK performed bioinformatics analysis and contributed to data visualization, edited manuscript, and approved final version, SKF performed DNA and RNA extraction, prepped DNA and RNA for sequencing submission, contributed to data visualization, edited manuscript, and approved final version, BMJ performed DNA and RNA extraction, prepped DNA and RNA for sequencing submission, contributed to data visualization, edited manuscript, and approved final version, MLL contributed to sequencing analysis, data visualization, edited manuscript, and approved final version, LP performed DNA preparation and submission for linked read sequencing, contributed to data analysis and data visualization, edited manuscript, and approved final version, JH performed formal statistical analysis, edited manuscript, and approved final version, LM performed data collection, edited manuscript, and approved final version, ELM provided supervision and contributed to project design, performed data collection, edited manuscript, and approved final version, JCB is responsible for project design, supervision, data analysis, data visualization, editing manuscript, and approval of final version.
Acknowledgements – None