ALFF:
In the comparison of ALFF value between amputation patients and control normal, we found that the ALFF value increased in Cingulate_Ant, ParaHippocampal, Hippocampus, Cingulate_Mid, Supp_Motor_Area, Insula, Putamen, Postcentral, Insula, Caudate, Hippocampus; decreased in Precuneus, Precentral, Occipital_Mid, Postcentral, Supp_Motor_Area, Paracentral_Lobule at 2months.(Fig. 1, Table 1)
Table 1
Brain region information of ALFF comparison between amputation patients and control normal at 2 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Cingulate_Ant_R | 100 | 3.295 | 3 | 27 | 0 |
| ParaHippocampal_R | 49 | 4.258 | 18 | 0 | -21 |
| Hippocampus_L | 189 | 4.257 | -21 | -27 | -12 |
| ParaHippocampal_L | 189 | 3.452 | -21 | -9 | -30 |
| Supp_Motor_Area_L | 8 | 3.729 | -3 | 21 | 54 |
| Insula_R | 19 | 3.583 | 42 | -9 | -9 |
| Putamen_L | 34 | 3.489 | -27 | -15 | 3 |
| Postcentral_L | 11 | 3.414 | -63 | -21 | 18 |
| Putamen_R | 16 | 3.334 | 27 | 3 | -3 |
Negative | Precuneus_R | 51 | -4.492 | 6 | -66 | 54 |
| Precentral_R | 97 | -4.332 | 42 | -12 | 57 |
| Precentral_L | 51 | -4.128 | -27 | 0 | 60 |
| Postcentral_R | 44 | -3.836 | 27 | -42 | 63 |
| Postcentral_L | 19 | -3.758 | -21 | -45 | 69 |
| Supp_Motor_Area_R | 42 | -3.705 | 3 | -15 | 72 |
The ALFF value increased in Putamen, Caudate, Hippocampus, Supp_Motor_Area, Lingual, ParaHippocampal, Precuneus, Cingulate_Mid, Cingulate_Ant, Parietal_Inf, Caudate; decreased in Paracentral_Lobule, Supp_Motor_Area, Precentral, Cuneus, Precuneus, Fusiform, Postcentral at 6 months. (Fig. 2, Table 2)
Table 2
Brain region information of ALFF comparison between amputation patients and control normal at 6 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Putamen_L | 88 | 4.993 | -21 | -3 | 3 |
| Caudate_R | 17 | 3.924 | 15 | 15 | 3 |
| Hippocampus_L | 36 | 3.804 | -30 | -21 | -21 |
| Supp_Motor_Area_L | 10 | 3.521 | -3 | 24 | 54 |
| Putamen_R | 11 | 3.386 | 24 | 6 | -6 |
Negative | Paracentral_Lobule_L | 903 | -6.797 | -15 | -36 | 63 |
| Supp_Motor_Area_R | 903 | -5.979 | 6 | -6 | 60 |
| Precentral_R | 30 | -5.233 | 48 | -3 | 42 |
| Lingual_R | 428 | -5.184 | 6 | -72 | -9 |
| Cuneus_R | 428 | -4.449 | 9 | -81 | 24 |
| Precuneus_R | 86 | -5.020 | 15 | -66 | 39 |
| Fusiform_R | 47 | -3.948 | 24 | 9 | -45 |
| Temporal_Inf_R | 27 | -3.210 | 42 | -57 | -9 |
The ALFF value increased in Cingulate_Ant, Cingulate_Mid, Caudate, Precuneus, Supp_Motor_Area, Frontal_Sup_Media, Insula, Thalamus, Putamen; decreased in Precentral, Postcentral, Supp_Motor_Area, Precuneus, Paracentral_Lobule. (Fig. 3, Table 3)
Table 3
Brain region information of ALFF comparison between amputation patients and control normal at 12 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Cingulate_Mid_R | 30 | 6.424 | 12 | -33 | 42 |
| Caudate_R | 18 | 5.593 | 21 | -3 | 18 |
| Precuneus_L | 28 | 4.674 | -9 | -69 | 60 |
| Caudate_R | 202 | 3.910 | 15 | 12 | 3 |
| Rectus_L | 202 | 3.835 | 3 | 30 | -18 |
| Pallidum_L | 25 | 4.531 | -21 | -3 | 0 |
| Supp_Motor_Area_L | 16 | 4.509 | -3 | 21 | 54 |
| Cingulate_Mid_L | 95 | 4.198 | -12 | -42 | 42 |
| Hippocampus_R | 14 | 4.155 | 36 | -30 | -6 |
| Frontal_Sup_Medial_R | 41 | 3.936 | 6 | 57 | 27 |
| Insula_R | 10 | 3.873 | 39 | -18 | 0 |
| Cingulate_Ant_R | 69 | 3.100 | 3 | 18 | 21 |
| Thalamus_R | 30 | 3.805 | 15 | -9 | -3 |
| Occipital_Mid_R | 24 | 3.614 | 51 | -66 | 24 |
| Putamen_L | 12 | 3.368 | -27 | -15 | 3 |
| ParaHippocampal_L | 32 | 3.362 | -27 | -39 | -12 |
Negative | Precentral_R | 29 | -6.454 | 30 | -21 | 63 |
| Lingual_R | 476 | -6.267 | 18 | -63 | -6 |
| Postcentral_R | 41 | -6.032 | 27 | -42 | 63 |
| Supp_Motor_Area_R | 25 | -5.477 | 3 | -9 | 57 |
| Fusiform_R | 102 | -5.381 | 24 | 3 | -45 |
| Lingual_L | 67 | -3.192 | -12 | -75 | -6 |
| Precuneus_R | 55 | -4.646 | 24 | -45 | 6 |
| Precentral_L | 26 | -4.588 | -21 | -18 | 63 |
| Paracentral_Lobule_L | 13 | -3.299 | -18 | -30 | 66 |
DC:
In the comparison of DC between amputation patients and control normal, we found that the DC value increased in Rolandic_Oper, Postcentral, Calcarine, Parietal, Cingulate_Mid, Frontal_Med_Orb, Frontal_Sup_Medial; decreased in Precuneus, ParaHippocampal, Caudate, Frontal_Inf_Tri, Precentral, Postcentral at 2months. (Fig. 4, Table 4)
Table 4
Brain region information of DC comparison between amputation patients and control normal at 2 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Rolandic_Oper_L | 11 | 4.459 | -63 | -3 | 12 |
| Postcentral_L | 10 | 3.603 | -63 | -21 | 27 |
| Cingulate_Mid_L | 27 | 3.373 | -6 | 12 | 39 |
| Frontal_Med_Orb_R | 12 | 3.290 | 9 | 42 | -12 |
Negative | Precuneus_R | 105 | -4.736 | 15 | -48 | 21 |
| ParaHippocampal_R | 105 | -3.788 | 18 | -39 | -6 |
| Caudate_R | 23 | -4.711 | 21 | 24 | -6 |
| Occipital_Mid_L | 18 | -4.159 | -27 | -78 | 0 |
| Frontal_Inf_Tri_R | 10 | -3.876 | 51 | 24 | 6 |
| Precuneus_L | 23 | -3.808 | -9 | -60 | 15 |
| Precentral_R | 10 | -3.426 | 60 | 9 | 15 |
The DC value increased in Cuneus, Calcarine, Postcentral, Parietal_Inf, ParaHippocampal; decreased in Cingulate_Mid, Precuneus, Hippocampus, Precuneus, ParaHippocampal, Caudate, Postcentral at 6 months. (Fig. 5, Table 5)
Table 5
Brain region information of DC comparison between amputation patients and control normal at 6 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Cuneus_L | 19 | 6.229 | 0 | -90 | 33 |
| Calcarine_R | 103 | 5.182 | 18 | -99 | 0 |
| Postcentral_L | 9 | 4.074 | -63 | -21 | 24 |
| ParaHippocampal_L | 14 | 3.290 | -18 | 3 | -33 |
| Postcentral_R | 6 | 3.138 | 45 | -42 | 63 |
Negative | Cingulate_Mid_R | 14 | -5.296 | 12 | -6 | 39 |
| Precuneus_R | 31 | -4.937 | 21 | -51 | 18 |
| Hippocampus_R | 15 | -4.685 | 27 | -21 | -15 |
| Precuneus_L | 15 | -4.310 | -3 | -54 | 69 |
| ParaHippocampal_R | 27 | -4.193 | 21 | -39 | -6 |
| Caudate_R | 5 | -4.040 | 21 | 24 | -6 |
| Cingulate_Mid_L | 11 | -3.602 | -12 | -33 | 42 |
| Postcentral_L | 6 | -3.025 | -30 | -33 | 54 |
The DC value increased in Lingual, Cuneus, Caudate, Fusiform, Postcentral, Frontal_Med_Or; decreased in ParaHippocampal, Precuneus, Cingulate_Post, Precuneus, Frontal_Inf_Tri, Frontal_Inf_Oper, Frontal_Med_Orb, Fusiform, Lingual, Putamen, Lingual at 12 months. (Fig. 6, Table 6)
Table 6
Brain region information of DC comparison between amputation patients and control normal at 12 months:
Contrast Name | | | MNI Coordinates |
| Region Label | Extent | t-value | x | y | z |
| | | | | | |
Positive | Lingual_R | 188 | 6.163 | 24 | -93 | -15 |
| Cuneus_R | 188 | 5.813 | 18 | -99 | 9 |
| Caudate_L | 12 | 4.725 | -12 | -3 | 15 |
| Fusiform_L | 23 | 4.308 | -21 | 3 | -45 |
| Postcentral_L | 10 | 4.030 | -21 | -30 | 78 |
| Parietal_Inf_R | 21 | 3.916 | 45 | -39 | 54 |
Negative | ParaHippocampal_R | 162 | -8.290 | 21 | -39 | -6 |
| Precuneus_R | 162 | -4.969 | 12 | -63 | 24 |
| Cingulate_Post_L | 56 | -6.593 | -6 | -39 | 12 |
| Precuneus_L | 25 | -5.595 | -6 | -60 | 15 |
| Frontal_Inf_Tri_R | 16 | -5.115 | 51 | 24 | 6 |
| Fusiform_R | 47 | -4.827 | 39 | -33 | -18 |
| Frontal_Inf_Oper_R | 20 | -4.427 | 36 | 6 | 27 |
| Putamen_R | 12 | -3.446 | 30 | -6 | 6 |