Background: To investigate the relationship between the expression of long-chain non-coding RNA (LncRNA) MALAT1 and radiotherapy effect in cervical cancer tissues.
Methods: Sixty cervical cancer patients with radiotherapy were selected and their tissue samples were collected. Siha cell lines and C-33A cell lines were used as MALAT1 high expression group and low expression group. These two cell lines were treated with 6 MV X-ray at 0, 2, 4, 6, 8, 10, and 12Gy, and the cell inhibition rate was detected by MTT assay at 24h, 48h, 72h and 96h after irradiation. Apoptosis rate was tested by flow cytometry and the percentage of autophagic cells was evaluated by the MDC method. Quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of MALAT1.
Results: The expression level of MALAT1 was higher in cancer tissues than in paired normal tissues in patients with cervical cancer (P <0.05). And the expression level of MALAT1 in cell line C-33A was significantly lower than that of cell line Siha. Besides, the cell inhibition rate in the MALAT1 low expression group was significantly higher than that in the high expression group, and the percentage of autophagic cells in MALTA1 low expression group was also higher (P<0.05). The percentage of autophagy cells in MALTA1 low expression group was higher than that in the high expression group (P<0.05).
Conclusion: MALAT1 plays an important role in cervical cancer and it is also a key point to interfere with the radiosensitivity of cervical squamous carcinoma cells.