The advent of low cost, high throughput DNA sequencing has led to the availability of thousands of complete genome sequences for a wide variety of bacterial species. Examining and interpreting genetic variation on this scale represents a significant challenge to existing methods of data analysis and visualisation. Starting with the output of standard pangenome analysis tools, we provide scripts to generate input files for a new open-source network analysis platform called Graphia. We describe the generation and analysis of interactive, 3D network graphs to explore the structure of a bacterial populations, the distribution of genes across a population, and the syntenic order in which those genes occur. Both the analysis and the visualisation are scalable to datasets of thousands of genome sequences. We anticipate that the approaches presented here will be of great utility to the microbial research community, allowing faster, more intuitive, and flexible interaction with pangenomes datasets, thereby enhancing interpretation of these complex data.