By analyzing the effects of LRAs under epigenetic constraints and considering the variability in NF-κB sites, it is evident that the activation of NF-κB alone is not sufficient to trigger latent HIV. The author used bioinformatics methods to analyze the viral proteins and their PBS. The results show that the amino acid sequence ARG of Gag proteins of HTLV-1, HTLV-2, STLV-1 and STLV-2 match their PBS GGGGGCTCG in the 3'-to-5' direction and that the amino acid sequence SPR of Gag proteins of HIV-1, HIV-2, SIV and FIV match their PBS GGCGCCCGA in the 3'-to-5' direction. Related studies have shown that the genomic Gag/Gag-Pol complex recruits the LysRS/tRNA complex. The selective packaging of the tRNA primer requires HIV-1 Gag and Gag-Pol, and an interaction between LysRS and Gag is observed in vitro. In HIV-1, Gag/LysRS interaction depends on Gag sequences within the CTD of CA around amino acids 283-363 and motif 1 of LysRS around amino acids 208-259. It should be noted that the amino acid sequence SPR of the Gag protein is located at amino acids 148-150 within the NTD of CA, specifically at the NTD-NTD interface 1. This study demonstrates the NTD of CA of the Gag protein matching with PBS, while relevant studies have shown interactions between the CTD of CA of the Gag protein and PBS. Currently, it can be confirmed that the CA of the Gag protein interacts with PBS, providing new options for future treatment approaches beyond LRAs.