In wide titanium strip cold rolling process, the high-order flatness defect is one of the most difficult problem to be solved. Based on finite element method, considering the anisotropic mechanical characteristics of titanium, an implicit integration calculation model of rolls-strip for 20-high mill was developed, which can simulate the dynamic rolling process. The model was used to analyze the adjustment characteristics of high-order flatness on the 20-high mill. The simulation revealed as the increasing of the 1# & 7# AS-U or 2# & 6# AS-U press adjustment, the high-order flatness defect was more aggravated; And as the increasing of 3# & 5# AS-U or 4# AS-U press adjustment, the high-order flatness defect was alleviated to some extent. In addition, the high-order flatness cannot be effectively adjusted by roll shifting. Finally, the industrial test showed that increasing 4# AS-U press adjustment can effectively relieve the high-order flatness defect.