In order to select the cells in vitro, identify the selected cells and trace the selected cells in small animal, we establish a multimodal imaging tool pProTG. We fused enhanced green fluorescent protein, Puromycin N-acetyltransferase and Firefly Luciferase to construct a multimodal imaging tool pProTG, which have ability of selecting the positive cells by puroR, identifying the selected cells by EGFP and imaging the cells in small animal by Fluc. Our established multimodal imaging tool can be used to select the targeted cells in vitro and trace the cells in vivo.