Monosodium Glutamate is a food additive well-known as a flavor enhancer being used extensively in food industries. Also, it has long been used as a cheap source of fertilizer owing to the presence of high nitrogen contents in it. Furthermore, it also shows genotoxic effects on plants. Hence, in the present study the genotoxic properties of Monosodium Glutamate had been explored to assess its potential as a mutagen. The objective was specifically to find out its potential in inducing polyploidy in plants. For this, a medicinal plant, Urginea indica Kunth with immensely high therapeutic value was selected. The effectiveness and efficiency of Monosodium Glutamate on Urginea indica Kunth was assessed based on the records obtained after treatments in two generations, viz. M1 and M2 Generations. Its effectiveness and efficiency on Urginea indica Kunth pave a way for its use as a potential mutagen which can induce genetic variability among the selected wild species of the plants with great ease. Moreover, the higher degree of polyploidy obtained in the M2 generation established the objective of the experiment, that Monosodium Glutamate could be used with controlled, adequate dose and maintained experiment setup in inducing polyploidy in plants.