Background
Cancer metabolism is associated with enhanced lipogenesis required for rapid growth and proliferation. However, the magnitude of dysregulation of diverse lipid species mostly remain to be characterized, particularly in understudied cancers such as ovarian clear cell carcinoma (OCCC). Further, formalin-fixed paraffin-embedded (FFPE) specimen represent valuable and readily available resources for laboratory investigations but are still underutilized for lipidomics studies. Here we implemented an integrated workflow to investigate lipidomic alterations in OCCC, utilizing FFPE specimen.
Methods
Lipid extraction of FFPE specimen was carried out by using 1-butanol and methanol in 1:1 (v/v), followed by targeted LC-MS/MS to identify the lipidomic changes in FFPE specimen from OCCC (n=14) as compared to uninvolved contralateral ovarian tissue (n=14). Immunohistochemistry (IHC) assays were implemented to evaluate the expression levels of stearoyl-CoA desaturase (SCD), the rate-limiting enzyme for the synthesis of monounsaturated fatty acids, in OCCC and control tissue specimen.
Results
We quantified 340 lipid species representing 29 lipid classes. We observed differential regulation of unsaturated lipid species belonging to several glycerophospholipid classes including ether-linked phospholipids, and trihexosylceramide species. We confirmed upregulation of SCD in OCCC by IHC assays.
Conclusions
By using lipidomic and IHC analysis of archival tissue samples, we were able to provide novel insights into the molecular alterations in OCCC. We show the feasibility of using FFPE specimen to carry out detailed lipidomic analysis of archival cancer samples.