The static metallic joints’ sealing performance is deeply impacted by the plastic deformation and the interfacial separation of the contact surfaces with self-affine roughness. The yield hardening effect, unavoidable at the contact interface, is of vital importance to the plastic deformation and the distribution of the interfacial separation. However, most of the previous research ignores the effect of the yield hardening, assuming that the contact surfaces are elastic-perfectly plastic. To address the problem, a novel analytic model for investigating the sealing performance under the effect of yield hardening has been developed in this paper. Utilizing the measured data of contact surfaces as input, the corresponding leakage rates are calculated. Besides, the contact stress distribution as well as the real contact area at the interface are also discussed. The sealing experiments are carried out accordingly, verifying that the proposed model owns the ability to predict the leakage rate under the effect of yield hardening.