Wild ginseng (Panax ginseng) adventitious root cultures were prepared by elicitation using methyl jasmonate and investigated further to find new secondary metabolites. Chromatographic fractionation of wild ginseng adventitious root cultures led to the isolation of eleven compounds. The chemical structures of isolated compounds were identified as four known flavanone derivatives (1–4), one new curcubinoyl derivative, jasmogin A (5) and six new curcubinoyl-flavanone conjugates, jasmoflagins A-F (6–11) by extensive spectroscopic analysis. Newly isolated curcubinoyl derivatives showed inhibitory activity against lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages. Curcubinoyl moiety is structurally similar with methyl jasmonate, which was used as an elicitor. Therefore, our present study suggested that elicitor might increase the content of active constituents and also contribute to the production of new metabolites by participation as precursors in biosynthesis. Taken together, plant cell cultures are useful not only for efficient production of active compounds but also development of new secondary metabolites.