Intrapartum transperineal ultrasound (ITU) is considered useful in judging fetal head descent; however, the inability to detect ischial spines on ITU has been a drawback to its legitimacy. The current study aimed to determine the anatomical location of ischial spines, which can be directly applied to ITU. Based on magnetic resonance imaging of 67 pregnant women at 33+ 2 [31+ 6-34+ 0] weeks gestation (median [interquartile range: IQR]), we calculated the angle between the pubic symphysis and the midpoint of ischial spines (midline symphysis-ischial spine angle; mSIA), which is theoretically equivalent to the angle of progression at fetal head station 0 on ITU, by determining spatial coordinates of pelvic landmarks and utilizing spatial vector analysis. Furthermore, we measured symphysis-ischial spine distance (SID), defined as the distance between the vertical plane passing the lower edge of the pubic symphysis and the plane that passes the ischial spines. As a result, mSIA was 109.6 ° [105.1–114.0] and SID 26.4 mm [19.8–30.7] (median, [IQR]). There was no correlation between mSIA or SID and maternal characteristics, including physique. Our results provide valuable evidence to enhance the reliability of ITU in assessing fetal head descent by considering the location of ischial spines.