Pharmaceutical product generally depends on environment controls during its storage and handling. Temperature mapping is essential and should be managed during entire product lifecycle with holistic approach. The purpose of this study was to determine the challenges during temperature mapping process in pharmaceutical product warehouses in Tanzania. The task analysis of temperature mapping process was carried out. As a result of the study, general requirements were formulated for the preparation; challenges were identified. Study shows majority of pharmaceutical workers lack of knowledge on temperature mapping, the use of local tool in temperature affecting the efficacy, safety and efficiency of the pharmaceutical product. The frequency of temperature mapping envisaged unsatisfactory. Hence, temperature mapping process in pharmaceutical industry should be recorded and reported to the manufacturer for further investigation and risk analysis. To address these challenges, implementation of a Quality Management System (QMS) must emphasize on the importance of temperature mapping and provides guidelines for its proper handling. The QMS should be integrated into the pharmaceutical warehouse management system and should involve all pharmaceutical workers, including medicine distributors and consumers. The concept of temperature mapping process is a critical that should be well-understood and implemented by pharmaceutical workers in pharmaceutical industry, through following proper guidelines and integrating QMS, and the challenges associated with temperature mapping can be minimized, and the quality of pharmaceutical products can be ensured.