The pH-sensitive chloride channels (pHCls) are unique chloride channels in invertebrates and have important roles in regulation of fluid secretion, food selection and intake. pHCls are widely expressed in insects, but little is known about their pharmacological properties and their sensitivity to metal ions. Herein, we isolated two cDNAs (Sfphcl1 and Sfphcl2) that encode SfpHCl1 and SfpHCl2 subunits from the fall armyworm Spodoptera frugiperda. The Sfphcl1 and Sfphcl2 demonstrated similar expression patterns with most abundant expression in the Malpighian tubules of the first instar larval stage. The cRNA of Sfphcl1 and Sfphcl2 were injected into Xenopus laevis oocytes singly or together in three different ratios of 1:4, 1:1 and 4:1 to characterize their function and investigate their zinc ion sensitivity. We found that SfpHCl1 and SfpHCl2 formed chloride channels in oocytes with a reversal potential that is consistent with chloride selectivity, but showed insensitivity to changes in external sodium concentrations. The extracellular pH shift and zinc-elicited inward currents in oocytes expressing SfpHCl1 and SfpHCl2 showed a similar EC50. These results provide the biophysical and pharmacological properties of pH-sensitive, zinc-gated chloride channels. Since the pHCls are present only in invertebrates, they may provide an ideal target for highly specific insecticides.