Fabrication, Design and In-vivo investigation of Mesoporous Silica Based Docetaxel Trihydrate Nanoparticles for Colonic Drug Delivery

doi:10.21203/rs.3.rs-3009807/v1

Background

Mesoporous silica-loaded docetaxel trihydrate nanoparticles are the potential to target drug delivery toward a specific region with high stability and predictable release at the target region. They have large surface areas and mesoporous structures with large pore volumes, leading to high bioavailability and therapeutic efficacy at the disease site. This study demonstrates how nanoparticles can be prepared using an emulsion technique.

Results

The ratios of eudragit S100 to eudragit L100 polymers, along with phosphatidylcholine, were varied according to the response surface methodology. DSC and FTIR studies showed that mesoporous silica particles were successful. All formulations had average particle sizes ranging from 70.65 to 143.01nm, with a range of zeta potential from 17.6 ± 026 to 21 ± 011. In-vitro drug delivery studies were achieved for all formulations with a zeta potential of 17.6 ± 026 to 21 ± 011. After oral administration of the docetaxel, plasma levels were measured in vivo bioavailability testing of docetaxel. Docetaxel nanosuspension had a significantly higher C_max amount than docetaxel micro-suspension (98.03 ± 23.40 ng/ml and 213.67 ± 72.21 ng/ml, respectively, with t_max 45 minutes). Docetaxel was more bioavailable in nanosuspension formulations, according to a bioavailability test of rats.

Conclusion

The results suggested that the mesoporous silica could be a great potential nanocarrier in colonic delivery with optimal drug content and controlled release docetaxel trihydrate.

Mesoporous silica based nanoparticles

Docetaxel Trihydrate

Response surface methodology

Evaluation parameters

In-vivo bioavailability study

Mesoporous silica nanoparticles have been intensively investigated to target the drug delivery toward a specific region with high stability, controlled and predictable release at the target region due to their honeycombs like mesoporous structure, large pore volume, porous structure, the high surface area, which leads to a high absorption rate, and enhanced bioavailability, suitable for poorly soluble drug and proteins and peptides [Sabio et al. 2019, Sargazi et al. 2022]. Recently mesoporous silica has been getting much more attention from researchers due to its low toxicity with high therapeutic efficacy and successful accumulation towards diseases site [Manzano et al. 2018, Samanta et al. 2019].

Targeting drug delivery in the colonic environment to treat colonic disease has proven difficult. Colon-targeted drug delivery refers to drugs that must be released at the target site to demonstrate therapeutic efficacy. There are fewer side effects and no premature drug release. The advantages of colon-targeted drug distribution systems over traditional drug delivery systems include the lower dosage of the drug that is sufficient to produce therapeutic efficacy in the targeted region, a reduced dosing frequency, and fewer systemic adverse effects [Mazumder et al. 2020, Naeem et al. 2020]. Many novel advancement technologies, such as carbon nanotubes, quantum dots, mesoporous silica drug delivery systems, and polymeric micelles, can enhance stability, accumulate drugs to the target area to show high therapeutic efficacy, and increase bioavailability [Muthukrishnan et al. 2022, Hossen et al. 2019].

Docetaxel trihydrate is a semi-synthetic second-generation taxane derivative active against breast, lung, and pancreatic cancers. It can also be used as a first-line treatment with cisplatin [Rarokar et al. 2017]. Docetaxel Trihydrate is highly hydrophobic, resulting in low systemic absorbtivity and low bioavailability, leading to high toxicity, hypersensitivity, musculoskeletal toxicity, and neurotoxicity. Nanoparticulate technology can overcome adverse reactions and adverse events in docetaxel trihydrate.

This study uses pH-dependent polymers to describe the design and evaluation of mesoporous silica particles entrapped with bioactive molecules docetaxel trihydrate. It targets the colonic environment to ensure maximum therapeutic efficacy towards the disease site. The statistical optimization of the mesoporous silica-loaded with docetaxel trihydrate nanoparticles was done. All formulations of mesoporous silica-entrapped docetaxel trihydrate nanoparticles were evaluated.

2.1. Materials

Docetaxel trihydrate was gifted from Fresenius Kabi Oncology Ltd, Kalyani, India. Phosphatidylcholine and Soya lecithin were purchased from Hi-Media. Eudragit S100 and Eudragit L100 were purchased from Evonik Rochm Pharma ltd. Others all chemicals and reagents used in the experiments were of analytical grade, followed by pharmaceutical standards.

2.2. Methods

2.2.1 Fabrication of mesoporous silica loaded docetaxel trihydrate nanoparticles

Docetaxel trihydrate nanoparticles containing mesoporous silica were prepared using oil-in-water emulsion techniques with minor modifications [Choi et al. 2015, Wang et al. 2020]. Table 1 lists the ingredients of various nanoparticle formulations. To chloroform containing docetaxel trihydrate, eudragit S100, or eudragit L100 polymers, an organic phase is created by measuring phosphatidylcholine. An organic phase contained approximately 40 mg of lecithin. The organic phase was added to an aqueous phase that contained 10 ml deionized water and 2 mg mesoporous silicon nanoparticles. After the solution had been heated to 65°C, it was stirred until it formed a milky phase. 1% v/v tween 20 was then added drop wise to make it opaque. It was then cooled to room temperature. Ultra-centrifugation was used to separate DTV-LNS from docetaxel at 8000 rpm. The centrifugal filter unit was used to reduce the temperature to 4⁰C.

Table 1

3² factorial design with the experimental response values for the various formulation of pH-dependent mesoporous silica loaded docetaxel trihydrate nanoparticles
Formulation code	Factors (Levels)		Responses
Formulation code	Ratio of eudragit S100 and L100 (mg) (A)	Phosphatidylcholine (mg) (B)	Entrapment efficiency (%)	Drug release at 6 hrs. (%)	Drug release at 10 hrs. (%)
F1	200 (+ 1)	60 (0)	78.38	34.31	73.39
F2	200 (+ 1)	40 (-1)	76.6	32.09	71.45
F3	100 (0)	60 (0)	64.77	24.24	57.16
F4	100 (0)	80 (+ 1)	59.6	20.03	53.38
F5	40 (-1)	40 (-1)	55.22	18.26	49.28
F6	100 (0)	40 − 1)	69.66	26.89	64.74
F7	200 (+ 1)	80 (+ 1)	75.44	31.87	68.48
F8	40 (-1)	80 (+ 1)	44.83	12.39	38.74
F9	40 (-1)	60 (0)	52.88	15.16	47.03

2.2.2 Characterization of mesoporous loaded docetaxel trihydrate nanoparticles

2.2.2.1 FTIR sand DSC studies

The interaction between drug and polymers of mesoporous silica-loaded docetaxel trihydrate nanoparticles was determined by using FTIR technique (Nicolet 380). The IR absorption peak of docetaxel trihydrate was taken in the range of 4000cm^− 1 to 400 cm^− 1 using the KBr disc method. The major peak was reported for the evaluation of purity [Majumdar et al. 2020, Kar et al. 2020].

DSC (Mettler star SW 1550) is used to characterize samples. The temperature range is 25–300 ⁰C, with a heating rate of 10 ⁰C per minute. Samples have an average weight of 2-4mg. The glass transition temperature and interactions between the drug and excipient are also characterized. The can is used as a reference to accurately weigh the samples into aluminium pans. DSC can also be recorded under liquid nitrogen at 5⁰C. Software [Mazumder et al. 2013] was used to calculate the enthalpies.

2.2.2.2 Particles size determination

Photon correlation spectroscopy was employed for measuring the particle size polydispersity (PDI) of docetaxel trihydrate-loaded nanoparticles. The temperature was 25 ⁰C below the fixed angles of 900C in disposable polystyrene curettes. The particle size polydispersity values were examined at the wavelength of 633 nm [Orlowski et al. 2018].

2.2.2.3 Scanning electron microscopy

Scanning electron microscopy helps to determine the uniformity of particle shape and size. Nanoemulsion was raised on the clear glass stub and sodium aurothimalate was used for the gold coating to visualize under a SEM at the magnification of 10,000 X [Thambiraj et al. 2019].

2.2.2.4 Encapsulation efficiency

The encapsulation efficiency of various formulations of mesoporous silica-loaded docetaxel trihydrate nanoparticles was examined by measuring the free drug concentration in the dispersion medium. 3ml of prepared nanoparticle dispersion was taken into the centrifuge tube and subjected to centrifuging at 16000 rpm for 30 mins. After Centrifugation, the supernatant layer of nanoparticle dispersion was subjected to filtering through Whatman filter paper. After washing and dilution of the supernatant layer of nanoparticles dispersion was determined through UV-visible spectrophotometer (1700, Shimadzu, Japan) at the wavelength of 229.5 nm. The drug loading efficiency of prepared nanoparticles was determined by the below-mentioned equation [Azizi-Lalabadi et al. 2019, Lv Y et al. 2018, Murugan et al. 2021].

2.2.2.5 Zeta potential

The Zeta potential of the different formulations containing Mesoporous silica-loaded docetaxel nanoparticles was determined by using Nano ZS90 Zetasizer and the measurement of effective electric charge on nanoparticles size was examined at 25°c with a fixed angle set to 173° to the reduction of multiple scattering [Verma et al. 2017, Shelake et al. 2018].

2.2.2.6 In-vitro release profile

In-vitro evaluation of mesoporous silica-loaded docetaxel nanoparticles formulations was done using a dialysis kit (Hi Media,India) and prepared formulations equivalent to 10 ml was placed into a dialysis bag filled with 100ml pH 6.8 (indicative stomach acid pH) and pH 1.2, with 37°C. At 50 rpm, the release medium was stirred. The release medium was blended at a rate of 50 rpm. Samples (1 ml) were reserved at predetermined intervals. A new buffer solution of one ml was added to maintain the sink condition. The mesoporous silica-loaded docetaxel-trihydrate nanoparticles were measured using a UV spectrophotometer at 229 nm. The cumulative percentage of drug released was estimated based on the pre-generated calibration curve. Each formulation was tested in triplicate [Orlowski et al. 2018, Poltavets et al. 2019].

2.2.2.7 Release kinetic

To evaluate the mechanism of silica-loaded, mesoporous docetaxel trihydrate, nanoparticles of docetaxel trihydrate were released from different formulations. To establish the mechanism of drug releases from nanoemulsions, the release exponent (n) was calculated using the korsemeyer peppas model.

2.2.2.8 Experimental design

The mesoporous docetaxel trihydrate nanoparticle was statistically optimized using 3² models based on factorial designs. The proportion of polymers such as eudragit S100 as well as eudragit L100 (A), and phosphatidylcholine (B) were evaluated as two independent factors and were classified into three levels, including low (-1), medium (0) as well as the highest (+ 1). The test batch was analyzed by utilizing independent variables at various levels that are suitable to optimize the statistical process of mesoporous silica-loaded pH-dependent docetaxel trihydrate nanoparticles. Three different response or dependent variables include the percentage of efficiency in loading of drugs (Y1) and the percentage of the release of medicines after 6 hours (Y2), and the percentage of drug loading after 10 hrs (Y3). The design and evaluation of the experimental data were performed using statease design Expert 11 trial version, shown in Table 1. The mesoporous silica-loaded docetaxel trihydrate nanoparticles were evaluated on the optimization of dependent variables on independent variables using an equation of polynomial as follows:

In this research, the variable Y is assumed to be the response variable. b₀ is considered the intercept as well as b₁, b₂, b₃, b₄ and b₅ are regarded as the coefficients of regression; while A and B are the response variables in the equation AB is considered to be an interaction between two variables. The validity of the model and all individual parameters of the response can be determined using an ANOVA One-Way model. The percent effectiveness of loading drugs of drug loading efficiency, the percent of the release of the drug at 6 hrs, and the percent of the release of drugs at 10 hours. The data are used to determine the targets specified as the independent variables [Sun et al. 2018].

2.2.2.9 Statistical analysis

The statistical optimizations of nanoparticles were calculated using design-expert software. The statistical analysis was achieved by ANOVA analysis. The p value was examined as statistically significant.

2.2.2.10 Accelerated stability study

The accelerated stability test of optimized formulations should be used to forecast the stability study by International Conference on Harmonization (ICH) Guidelines with little abatement. The optimized formulation should be placed in ambient colour vials and sealed with aluminium foil to allow for the short-term, accelerated stability test at 40 ± 5% RH and 75 ± 5% RH. The sample was tested over three months. At various intervals, the sample was collected and examined to determine stability. The parameters included physical appearance clarity, clarity, pH, and entrapment efficiency [Mazumder et al. 2021].

2.2.3 In vivo bioavailability study

This study examined the pharmacokinetics of a nanosuspension of docetaxel trihydrate. It was used for measuring docetaxel plasma levels following oral administration. This was done in an experiment that involved a two-way crossover design. Here, six healthy male rats were used (200–250 grams). It was accomplished by animal ethical standards [Tazeze et al. 2021]. As a test sample, the rats received docetaxel nanosuspension each session and the pure drug docetaxel at a specific time. The pure drug and formulation were administered orally to each animal at 1.5 mg/kg using an oral feeding tube.

2.2.3.1 Pharmacokinetics

For the study, we used the HPLC (Shimadzu Corp. HPLC). The mobile phase was made using a 30:70 phosphate buffer and methanol at a 1 ml/min flow rate. The retro-orbital puncture technique collected 0.5ml of blood before dosing (0 hrs) and at 0, 0.5, 1, and 2 hr. Samples were centrifuged for 10 minutes at 5500 rpm. Blood plasma was preserved and collected at 20 ⁰C [Mazumder et al. 2022]. Mix 0.1 ml of blood with 0.5 ml of acetonitrile to extract the drug and let it sit for 10 minutes. Transfer 150 ul plasma and 50mg IS into 1.5 ml centrifuge tubes. Deproteinization is completed after adding 300ml of Acetonitrile, shaking each sample for five minutes, and centrifuging the samples at 6600rpm for five minutes. Micropipettes were used to collect the supernatant solutions of each sample. The filtrate was then passed through a filter of 0.45 microns. The HPLC was used to introduce 20 microliters of the top pure layer.

2.2.3.2 Pharmacokinetics analysis

The pharmacokinetic studies were done by the plot between drug concentration and time to determine the pharmacokinetic parameters, such as C_max, t_max, AUC_0 − t, AUC_{0 − inf}, t_1/2, and K_el [Ghelicha et al. 2019]. Various pharmacokinetics constraints estimated the relative bioavailability of docetaxel, and statistical studies were presented by the mean ± SD using SPSS 13 software.

3.1 Fabrication of mesoporous silica loaded docetaxel trihydrate nanoparticles

Mesoporous silica loaded docetaxel trihydrate nanoparticles were formulated by emulsion technique with negligible moderation employing the polymer ratio of Eudragit L100 and Eudragit S100 using response surface method (Table 1) [Luna et al. 2021, Narayan et al. 2018]. Docetaxel trihydrate used as model drug. The nanoparticles were prepared with organic phase containing phosphaditylcholin added to chloroform dispersed to aqueous phase containing 10 ml of deionized warm water and 2mg mesoporous silica nanoparticles. The preparations were heated at 65⁰C with continuous stirring. In this study, all nanoemulsions contained tween 20, an emulsifying agent for the preparation of stable form added drop wise and cooled down at room temperature.

3.2 Characterization of mesoporous loaded docetaxel trihydrate nanoparticles

3.2.1 FTIR sand DSC studies

The FTIR spectrum of docetaxel trihydrate, eudragit S100, and eudragit L100 are shown in Fig. 1. Docetaxel Trihydrate has a melting point in the range of 114.52 °C. The thermo gram peak of the drug (Docetaxel trihydrate) and polymers (Eudragit S100 and L100) were described in Fig. 2 [Joshi et al. 2019].

3.2.2 Particles size determination and polydispersity index (PDI)

The particle size of different formulations containing Eudragit loaded mesoporous silica loaded docetaxel trihydrate nanoparticles is mentioned in Table 2. Particle sizes of mesoporous silica-loaded docetaxel trihydrate nanoparticles were observed in between the range of 72.81 nm to 110.97 nm [Ibrahim et al. 2020].

Table 2

Zeta potential and particle size of different formulations (mean ± S.D; n = 3)
Formulation code	Zeta Potential (mv)	Particle size (nm)
F1	-21.1 ± 0.11	73.08 ± 1
F2	-19.3 ± 0.23	72.81 ± 1.2
F3	-18.9 ± 0.25	84.51 ± 1
F4	-18.7 ± 0.23	92.88 ± 3
F5	-18.6 ± 0.01	86.31 ± 2
F6	-18.4 ± 0.12	83.61 ± 2
F7	-18.0 ± 0.14	104.85 ± 1.5
F8	-17.8 ± 0.24	110.97 ± 1
F9	-17.6 ± 026	101.52 ± 2

3.2.3 Scanning electron microscopy (SEM)

The single emulsion preparation process was successful in producing Mesoporous silica-loaded docetaxel trihydrate nanoparticles employing tween 20 as the surfactant in the formulation and analysis of the SEM image demonstrated the morphological characteristics of the nanoparticles and shown in Fig. 3 [Pandita et al. 2021].

3.2.4 Encapsulation efficiency

Various formulations of drug entrapment efficiency of mesoporous silica-loaded docetaxel trihydrate nanoparticles were observed within the range of 44.83 ± 0.5% to 78.38 ± 0.25% [Kassem et al. 2019].

3.2.5 Zeta potential

Zeta potential values of various formulations containing mesoporous silica loaded docetaxel trihydrate nanoparticles were mentioned in the Table 2. The zeta potential of mesoporous loaded docetaxel trihydrate nanoparticles was observed within the range of -18.0 ± 0.14 mv to -21.1 ± 0.11 mv, considering that prepared nanoparticles had enough charge at their surface which prohibits exhibition due to repulsion of electric charges [Sawyer 2009].

3.2.6 In-vitro release profile

The in-vitro dissolution summary [Peng et al. 2020] of different formulations containing mesoporous silica-loaded docetaxel trihydrate nanoparticles is mentioned in Fig. 4. Through the data analysis, the F1 formulation containing 60 mg of phosphatidylcholine and a 1:0 ratio of Eudragit S100 and L100 was released at 73.39% at 10 hours.

3.2.7 Release kinetic

The in-vitro dissolution data of mesoporous silica loaded docetaxel trihydrate formulation was suitable for various kinetic equations and the correlation coefficient (R²) value was calculated from different kinetics models and through correlation coefficient (R²) value the release mechanism was calculated in Table 3.

Table 3

Different release kinetic of mesoporous silica nanoparticles
Formulation	Zero order		First order		Higuchi Mechanism		Korsmeyer Peppas
Formulation	R²	n	R²	n	R²	n	R²	n
F1	0.949	7.586	0.909	-0.063	0.884	31.28	0.941	1.135
F2	0.944	7.914	0.913	-0.062	0.890	32.85	0.957	1.324
F3	0.940	6.540	0.921	-0.042	0.867	26.71	0.938	1.494
F4	0.898	6.041	0.880	-0.038	0.834	24.88	0.921	1.401
F5	0.908	4.423	0.854	-0.026	0.839	18.17	0.974	1.380
F6	0.940	7.259	0.911	-0.051	0.884	30.09	0.971	1.522
F7	0.936	7.813	0.908	-0.059	0.876	32.31	0.952	1.394
F8	0.881	4.986	0.856	-0.027	0.806	20.38	0.998	1.836
F9	0.909	5.267	0.896	-0.030	0.830	21.51	0.918	1.467

3.2.8 Formulation optimization by experimental design and statistical analysis

The mesoporous silica-loaded docetaxel trihydrate was optimized by 3² full-factorial designs (Table 1). In this full factorial design, the ratio of Eudragit polymers (S100 and L100) and phosphatidylcholine were used as dependent variables based on various trial batches responses and varied at three different levels low (-1), medium (0), and high (+ 1). The three different response variables such as the percentage (%) of drug loading efficiency, percentage (%) of drug release at 6 hrs and percentage (%) of drug release at 10 hrs. The different investigated response is denoted using a quadratic equation as follows:

From the above-mentioned equation, the high value of the correlation coefficient signifies the investigated responses are associated strongly with the factors studied during the experiment. The ANOVA results shown in Table 4. The model simplification of the quadratic above-mentioned equation was considered for eliminating the non-significant (p > 0.05) terms.

Table 4

Summary of ANOVA for response surface quadratic models
Source	Sum of square	Degree of Freedom	Mean Square	F-value	P-value prob > F
Entrapment efficiency (%)
Model	1048.28	2	524.14	46.15	0.0002
A	970.45	1	970.45	85.45	< 0.0001
B	77.83	1	77.83	6.85	0.0397
AB	165.38	1	165.38	0.5546	0.0510
A²	1.28	6	11.36	0.0043	0.0951
B²	0.5408	1	0.5408	0.0018	0.0968
Drug release at 6 hours (%)
Model	479.78	2	239.89	60.24	0.0001
A	451.83	1	451.83	113.47	< 0.0001
B	27.95	1	27.95	7.02	0.0381
AB	1021.44	1	70.81	0.5408	0.0515
A²	2.52	1	2.52	0.0193	0.0898
B²	0.9339	1	0.9339	0.0071	0.9380
Drug release at 10 hours (%)
Model	1097.12	2	548.56	52.08	0.0002
A	993.20	1	993.20	94.29	< 0.0001
B	103.92	1	103.92	9.87	0.0200
AB	122.77	1	122.77	0.3766	0.05828
A²	0.2813	1	0.2813	0.0009	0.9784
B²	0.0968	1	0.0968	0.0003	0.9873

The Linear correlation plots of actual and predictable results with the corresponding residual plot of entrapment efficiency (%), drug release at 6 hours (%), and drug release at 10 hours (%) are presented in the figure no 1–3, and corresponding residual plots are show a scatter between residual vs predictable value of entrapment efficiency (%), drug release at 6 hours (%) and drug release at 10 hours (%) presented in the Fig. 5.

Ratio of eudragit S100, L100, and phosphatidylcholine as the independent variables impacts on response variables such as entrapment efficiency (%), drug release at 6 hours (%), and drug release at 10 hours (%) were explicated using three-dimensional response surface plot and two-dimensional contour plot [40] which related with entrapment efficiency (%)(Fig. 6). The response surface design also confirms that the linear curve of the ratio of eudragit s100 and L100 was almost flattered was seen for the phosphatidylcholine (Fig. 7).

The contour plot (Fig. 6) of drug release at 6 hours (%) factors A (ratio of eudragit S100 and L100) and B (phosphatidylcholine) showed that linearity increased of drug release at 6 hours (%) with an increase in the ratio of eudragit s100 and L100 (A) and phosphatidylcholine (B) at the low level of (-1) of phosphatidylcholine (B) the drug release was increased (p < 0.05) significantly when A increase from − 1 level to + 1 level. The response surface design (Fig. 7) also relating to the drug release at 6 hours (%) designated that linear curvature on the ratio of eudragit S100 and eudragit L100 (A) and phosphatidylcholine (B) and considered as both A and B impacts release rate at 6 hours (%).

The contour plot (Fig. 6) of drug release at 10 hours versus independent factors involved in such ratio of eudragit S100 and L100 (A) and phosphatidylcholine (B) that the linearity increases drug release with both independent variables. In another way, a non-linear curve slightly increases the percentage of drug release at 10 hours was identified with an increase in the ratio of eudragit S100 and eudragit L100 (A). At the high (+ 1) and low (-1) level of A, drug release at 10 hours was increased significantly (p < 0.05) as B was increased from low level (-1) to high (+ 1) (Fig. 7).

After the determination of polynomial equations for the independent as well as the dependent variables, to get an optimized formulation with the desired responses the combination was statistically optimized for all three different responses. Trading off various responses, the desired responses were restricted as 44.83%≥ entrapment efficiency (%); 34.31 ≥ drug release at 6 hours, and 73.39 ≤ drug release at 12 hours. After all comprehensive evaluation of the responses with adequate observation, the formulation containing 60 mg of phosphatidylcholine and 1:0 ratio of Eudragit S100 and L100 was the maximum desired requisites. After examination of all the responses, the F1 formulation was considered an optimized formulation.

3.2.9 Accelerated stability study

p^H dependent polymer gated mesoporous silica nanoparticles of docetaxel trihydrate nano-emulsion formulation showed a good stability during the period of 3 months [Iyer V et al. 2015]. The appearance of formulation (Table 5) was yellowish in colour and cloudy. The pH was also found to be within the range of 6.4–6.8, which is desirable for colon targeting.

Table 5

Result of various parameters of optimized formulation at different time intervals under accelerated stability condition
Time period (month)	Visual appearance	Clarity	Entrapment efficiency (%)	pH
0	Yellowish	Cloudy	77.24 ± 0.36	6.4
1	Yellowish	Cloudy	78.11 ± 1.01	6.7
2	Yellowish	Cloudy	76.78 ± 0.62	6.5
3	Yellowish	Cloudy	77.89 ± 1.31	6.8

3.3 In vivo bioavailability study

Docetaxel was measured in plasma following oral administration using the in vivo bioavailability test of docetaxel. Comparing pure drug microsuspension and docetaxel nanosuspension demonstrated enhanced bioavailability (Table 6& Fig. 8). There was a Cmax of docetaxel following orally administered docetaxel microsuspension was 98.03 ± 23.40 ng/ml and then it took 45 minutes to reach tmax and docetaxel nanosuspension had C_max, and the tmax was 213.67 ± 72.21 ng/ml and 45 min respectively. The Cmax quantity of docetaxel nanosuspension (Formulation) compared with docetaxel microsuspension (Control) of 98.03 ± 23.40 ng/ml and 213.67 ± 72.21 ng/ml [Jackson et al. 2020]. The AUC _0−inf was significantly elevated in docetaxel based nanosuspension, compared to microsuspension containing docetaxel ranging from 9967.14 ± 2386.35 in ng/ml up to 31955.93 ± 11145.17 ng/ml.

Table 6

Comparison of pharmacokinetic data of docetaxel nanosuspension (Formulation) and pure docetaxel powder (Control) as microsuspension
Pharmacokinetic parameters	Pure docetaxel microsuspension (Control)		Docetaxel nanosuspension (Formulation)
C_max (ng/ml.)	Mean	98.03	Mean	213.67
C_max (ng/ml.)	± S.D.	23.40	± S.D.	72.21
t_max (min)	Mean	45.00	Mean	45.00
t_max (min)	± S.D.	0.00	± S.D.	0.00
AUC _0−t (ng. min/ml.)	Mean	9627.76	Mean	28345.41
AUC _0−t (ng. min/ml.)	± S.D.	2298.52	± S.D.	9687.11
AUC _0−inf (ng. min/ml.)	Mean	9967.14	Mean	31955.93
AUC _0−inf (ng. min/ml.)	± S.D.	2386.35	± S.D.	11145.17
k_el (min^− 1)	Mean	0.007	Mean	0.005
k_el (min^− 1)	± S.D.	0.000	± S.D.	0.000
t_1/2 (min)	Mean	93.18	Mean	133.34
t_1/2 (min)	± S.D.	3.47	± S.D.	7.61

The formulations were designed by 3² full-factorial designs using eudragit polymers (S100 and L100) and phosphatidylcholine as dependent variables giving relevant results compared to the conventional product.

There are several vibrational peaks of docetaxel trihydrate were observed in FTIR. The NH stretching of alkanes, C-O stretching vibration, N-H plane bonding vibrations, and C = O stretching were observed at 3432 cm^− 1, 1782 cm^− 1, 1635 cm^− 1, and 720 cm^− 1. With the drug and polymers mixture peaks were observed at 3375 cm^− 1, 1772.61 cm^− 1, 1684.85 cm^− 1, and 707.89 cm^− 1 [Zhuang et al. 2020]. The two-graph of stretching vibrational peaks were matched each other showing that the drug sample used is pure and stable and there are no such interactions were found with drug and Polymers. The DSC thermogram peak of the drug and polymers were found in the range of 115.64 °C. The two graphs of the thermogram were matched with each other and conclude that there is no such interaction found between the drug and polymers.

pH-dependent polymer gated mesoporous silica-loaded docetaxel trihydrate nanoparticles had a narrow size distribution in addition to a significant difference in the distribution between pH-dependent polymer gated mesoporous- silica nanoparticles of docetaxel trihydrate and drug-free pH-dependent polymer gated mesoporous silica loaded docetaxel trihydrate nanoparticles was observed.

The SEM of nanoparticles have smooth with a uniform spherical shape and it also approved the particle size found in the particle size analysis.

The poor encapsulation efficiency of mesoporous silica-loaded docetaxel nanoparticlesis due to low solubility and it has been examined that nanoparticles are increased drug encapsulation efficiency and reduce drug leakage as well as prolong residence and accumulation of drugs at target sites. The drug loading efficiency (%) was enhanced significantly (p < 0.05) as the ratio of Eudragit S100 and L100 and phosphatidylcholine were increased.

The zeta potential range of the nanoparticles is within the range of ± 30 mv, it shows less coalescence and stable nanoparticles due to electrostatic repulsion between particles. The significance of the zeta potential value indicates the stability of the nanoparticles with less coalescence of formulated mesoporous silica-loaded docetaxel trihydrate nanoparticles.

The in-vitro dissolution summary of mesoporous silica loaded docetaxel trihydrate has significant (p < 0.05) enhancement due to the large surface area leading to small particle sizes and polymers (Eudragit S100 and L100) as well as surfactant played an important role in the release rate. This evidence shows that the potential release of mesoporous silica-loaded docetaxel trihydrate indicates the enhancement of therapeutic efficacy through the predictable release of docetaxel trihydrate at the target site. The release pattern of mesoporous silica loaded docetaxel trihydrate followed zero-order kinetics and also followed the Korsmeyer Peppas model indicated from the highest R² value and n exponent.

The RSM study using design expert software helps to determine individual main factors and interaction factors using the quadratic equation. The ANOVA result clearly states that both the factors such as the ratio of eudragit S100 and L100 and phosphatidylcholine had a significant value (p < 0.05) effect on entrapment efficiency (%), drug release at 6 hours (%), drug release at 10 hours (%).Results shows that non-linearity decreased the entrapment efficiency with increase independent variables of phosphatidylcholine and entrapment efficiency linearly increase with the ration of eudragit S100 and L100 increase, where A mainly increase from − 1 to + 1 level. From the response surface plot, entrapment efficiency was more dependent on the ratio of eudragit S100 and L100 (A) compared to phosphatidylcholine. The results of the response surface plot of drug release showed that greater curvature toward the ratio of eudragit s100 and L100 (A) and flattering of the curve toward phosphatidylcholine as both independent variables impact the release rate.

There is no significant change of clarity and physical appearance was observed by the stability study. The centrifuge test also showed that the formulation had good physical stability. The good stability might be due to low particle size and effect of tween 20. No degradation of docetaxel trihydrate in optimized formulation was also observed.

In vivo studies shown, Cmax quantity of docetaxel significantly increased when using nanosuspension (Formulation) compared with docetaxel microsuspension and arise in relative bioavailability to the dose was determined to be 3 times. The bioavailability study of rats showed that docetaxel's bioavailability was higher for the nanosuspension formulation.

The mesoporous silica loaded docetaxel trihydrate nanoparticles were successfully applied to the oil in water emulsion and formulation was statistically optimized by 3² factorial designs, the investigation was done on independent variables (ratio of Eudragit S100 and L100 and phosphatidylcholine) and dependent variables (entrapment efficiency, drug release at 6 hours and drug release at 10 hours). Upon all the comprehensive evaluation of formulation, it was found the formulations, F1 containing 60 mg of phosphatidylcholine and a 1:0 ratio of Eudragit s100 and L100 full filled the maximum desired requisites. The bioavailability of Docetaxel Trihydrate is very poor. This can be overcome by formulating Nanoparticles, which can release the drug in a controlled pattern. The polymer concentration plays a major role in the design of control release of Nanoparticles. A higher concentration of Phosphatidylcholine provides good control of the release of the drug for an anticipated period. Systematic studies using the design of experiment optimization could surmount the hiccup of balancing coveted drug release patterns using the polymer combination. The choice of experimental design, i.e., central composite design was found to be highly appropriate as it can detect any non-linearity in factor response relationship with minimal expenditure of development effort and time. Docetaxel was measured in plasma following oral administration using the in vivo bioavailability test of docetaxel. Comparing pure drug microsuspension and docetaxel nanosuspension demonstrated enhanced bioavailability. The rise in relative bioavailability to the dose was determined to be 3 times. The bioavailability study showed that docetaxel's bioavailability was higher for the nanosuspension formulation. The optimized formulation exposited excellent controlled release characteristics vouching for the success of the experimental approaches followed.

PDI: Polydispersity index; SEM: Scanning electron microscopy; FTIR: Fourier transform infrared spectroscopy; DSC: Differential scanning colorimeter; RSM: Response surface method; C_max: Maximum plasma concentration; t_max: time to attain maximum concentration; K_el: plasma elimination rate constant; HPLC: High performance liquid chromatography

Ethics approval and consent to participate

The author’s assert that all procedure contributing to this work comply with the ethical standards of the Institutional Animal Ethics Committee “TAAB Biostudy Services,” 69 Ibrahimpur Road, Jadavpur, Kolkata–700032, India and ethical approval number is 1938/PO/Rc/S/17/CPCSEA.

Consent for publication

Not applicable.

Availability of data and materials

All necessary data generated or analyzed during this study are included in this published article. Any additional data could be available from the corresponding author upon request.

Competing interests

The authors declare that they have no competing interests

Funding

The authors have no funding to report.

Authors’ contributions

We declare that this work was done by the authors named in this article: SM, MM,AB and RM conceived and designed the study. MM,AB and AKK carried out the laboratory work, collected and analyzed the data and RM drafted the manuscript. SM supervised the work and MM assisted in the data analysis. All authors have read and approved the final manuscript.

Acknowledgements

Authors wish to give thanks to Calcutta Institute of Pharmaceutical Technology, Howrah, West Bengal, India and TAAB Biostudy Services, 69-Ibrahimpur Road, Jadavpur, Kolkata, West Bengal, India, given research laboratory to carry out this project work and also thanks to Gitanjali College of Pharmacy, Birbhum, West Bengal, India for help in completion of the research project work.

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