Callibaetis (Cunhaporanga) nigracyclus Cruz, Salles & Hamada, 2014
Sampling site: Itamacaoca Stream
Collection number: CINCAA 145, 146, 147
Comments: Nymphs were sampled in the Itamacaca Stream and reared in the laboratory until the emergence of male and female imagos. They were identified based on the nymphal description (Cruz et al. 2014) and the key to identify nymphs of Callibaetis from South America (Cruz et al. 2017) using the following characters: female fore wing with single marginal intercalary vein, with area around bulla and some spaces on outer margin pigmented (Fig. 2B); hind wing venation hyaline (Fig. 2C); turbinate eye of male basally constricted (Fig. 2D); distal margin of the labrum with medial emargination (Fig. 3A), glossa subegual in length to the paraglossa (Fig. 3B), labial palp with spine-like setae (Fig. 3B), hind claw without tiny spines on the surface (Fig. 3C).
In the present work, the species is reported for the first time from the State of Maranhão. The new record also represents a geographic extension, as the species was recorded only in northern Brazil, in the states of Pará and Amazon (Cruz et al. 2014).
Figure 2. C. nigracyclus: A – Dorsal view of nymph, scale bar 500 µm; B- lateral view of female imago, scale bar 500 µm; C - male hind wing, scale bar 500 µm; D - partial view of thorax and head of male imago, scale bar 200 µm.
Figure 3. C. nigracyclus. Selected nymphal structures: A, labrum, scale bar 100 µm; B, labium, scale bar 100 µm; C, hind tarsal claw, scale bar 10 µm.
Cloeodes auwe Salles & Batista, 2004
Sampling site: Riacho Feio Stream
Collection number: CINCAA 136
Comments: Cloeodes auwe was described by Salles et al. (2004) and the description was based only on nymphs from Mato Grosso, the adults of the species were only described eight years later by Massariol et al. (2013), who described the female and male of the species. Later, Kluge (2017) reported its presence in Peru. The species was identified based on the following characters: characteristic spot on the nymph’s first abdominal terga (Fig. 4A); hind wing pads absent (Fig. 4A); femora with two spatulate spines apically (Fig. 4B); tarsal claws 0.5 times length of tarsi; and maxillary palp longer than galea-lacinia (Fig. 4C). The species and genus are reported for the first time from the State of Maranhão, also presenting a geographic extension of northeast Brazil.
Figure 4. Nymph of C. auwe: A - dorsal view, scale bar 1 mm; B - partial view of middle leg with arc of long setae (arrow), scale bar 50 µm; C – maxilla, scale bar 50 µm.
Hermanella mazama (Nascimento, Mariano & Salles, 2012)
Sampling site: Munin River
Collection number: CINCAA 134
Comments: The species H. mazama, described by Nascimento, Mariano and Salles (2012) was first allocated in the genus Needhamella Domínguez & Flowers, 1989 and later transferred to the current genus by Lima et al. (2012).
The species was identified by the following characters: clypeus without medial projection; gills translucent, with a basal and median grayish band; terga yellowish brown (Fig. 5A); galea-lacinia with prominent tusk on inner apical margin (nearly half the length of galea-lacinia) (Fig. 5B); tarsal claw devoid of accessory denticles (Fig. 5C).
The species is being registered for the first time from the State of Maranhão, which also consists an important geographic extension. The species was previously reported only from the states of Espírito Santo and Bahia, with an extension area (sensu IUCN) of less than 20.000km2 (ICMBio 2023).
Figure 5. Nymph of H. mazama: A - dorsal view, scale bar 1 mm. Selected mouth parts of the nymph: B – maxilla, scale bar 20 µm; C- tarsal claw, scale bar 20 µm.
Among the three species considered herein C. nigracyclus represent a new record in the State, while C. auwe and H. mazama represent new records at the species and generic level. All these records represent a geographic extension for northeastern Brazil (Fig. 6).
Figure 6. Distribution map of C. nigracyclus, C. auwe and H. mazama. Triangles represent distribution of C. nigracyclus, circle represent distribution of C. auwe and square represent H. mazama. Club represent the new records of three species. Colors in map represent biomes.