Real-Life Variability of Corneal Epithelium Thickness in Photorefractive Keratectomy

doi:10.21203/rs.3.rs-34411/v1

PURPOSE: To evaluate the real-life epithelial thickness (ET) as measured intraoperatively by optical coherence pachymetry (OCP) in myopic eyes undergoing alcohol-assisted photorefractive keratectomy (PRK).

METHODS: A retrospective review of patients who underwent alcohol-assisted PRK was performed. Data were abstracted on age, gender, contact lens (CL) wear, preoperative refractive errors, keratometry, topographic and ultrasonic pachymetry, and intraoperative OCP measurements before and after epithelium removal. The central ET was calculated by subtracting OCP measurement after epithelium removal from the OCP measurement prior to epithelium removal.

RESULTS: The study comprised of 140 consecutive eyes from 70 patients. The mean age was 27.29 ± 6.57 years, 51.4% were females. CL was used in 80 eyes (57.1%). The mean sphere and spherical equivalent were -3.69 ± 2.74D and -3.36 ± 2.76D, respectively. The mean intraoperative ET was 59.49 ± 19.93µm (range, 15-150µm). Fifty-four percent of the eyes had an ET measurement above or below the range of 40-60µm. ET was significantly higher in the second operated eye compared to the first operated eye (p=0.006). There was no significant difference in thickness between genders (p=0.29) or association to CL-wear (p=0.11), and no correlation to patient age (p=0.18, r_p=0.13), refractive errors (p>0.11, r_p=-0.02-0.14), nor keratometry (p>0.64, r_p=-0.01-0.02).

Conclusion: The real-life assessment of intraoperative ET in alcohol-assisted PRK showed a high variability of the central corneal epithelium, with a significant difference between the first and second operated eyes. This difference may have implications when the epithelium is not included in the surgical planning in surface ablation.

Ophthalmology

Corneal epithelium

Epithelium thickness

Photorefractive keratectomy

Optical coherence pachymetry

The corneal epithelium has been lately recognized to have a distinct profile.[1] The epithelium plays an important role in the corneal power and accounts for an average of 1.03 diopters;[2] it has an accepted average thickness of approximately 50-60 µm.[1, 3] Several factors have been shown to influence the central epithelial thickness profile, and while controversial in the literature, they include age,[4, 5] gender,[4, 6] ethnics and refractive errors.[6, 7] Pathological conditions, such as contact lens (CL) wearing,[8] dry eye,[9, 10] and keratoconus,[11] have been also shown to cause alterations of ET.

Numerous novel imaging modalities, such as high-frequency scanning ultrasound biomicroscopy,[3] confocal microscopy,[12, 13] and optical coherence tomography (OCT),[10, 14, 15] have been developed in the last decade. These devices map the epithelium by differentiating it from the underlying corneal layers, and are utilized in the diagnosis and follow-up of various corneal disorders as well as in the preoperative evaluation of refractive-surgery candidates. The optical coherence pachymetry system (OCP, Heidelberg Engineering, Lübeck, Germany) is integrated in the SCHWIND Amaris excimer laser, providing non-contact continuous measurements of the central corneal thickness during refractive procedures. It allows a real-life intraoperative monitoring of the central corneal thickness throughout the entire surgery. The OCP has been shown to have high reproducibility of intraoperative corneal changes, such as the flap and the residual stromal thickness.[16]

During photorefractive keratectomy (PRK), the treatment is performed on the Bowman’s layer and corneal stroma, while the epithelium is removed either manually or alcohol-assisted. Therefore, the epithelium does not play a role in the refractive outcome. Recently, transepithelial PRK (t-PRK) has been gaining popularity due to its being a single-step surgery composed of a fixed predefined epithelial ablation of 55 μm at the center of the cornea and 65 μm at the periphery, followed by stromal ablation in a continuous profile. However, individual variations and intraoperative changes of ET may lead to incomplete removal or overtreatment of the epithelium that may affect the refractive outcome.

To the best of our knowledge, the intraoperative real-life central corneal epithelium measurement has not been previously reported. The purpose of our study was to evaluate the intraoperative ET in alcohol-assisted PRK.

Patient and study design

This study was a retrospective evaluation of eyes that underwent bilateral simultaneous alcohol-assisted myopic PRK between October 2018 through May 2019. Indications for the PRK included eyes with corneal thickness greater than 480 µm, no contraindications for laser vision correction, and refractive errors indicated clinically being correctable with an aspheric PRK profile. We included patients with complete pre- and intraoperative data as described below and excluded from the analysis patients who underwent unilateral PRK or missing complete data on both eyes. Patients received a detailed written informed consent form prior to surgery. The study was approved by the Institutional Review Board at the Sheba Medical Center and complied with the Declaration of Helsinki.

Preoperative examination

A detailed ophthalmic and systemic history was obtained. CL wear was discontinued at least 10 days, depending on CL type, prior to the preoperative evaluation and surgery. Preoperative examination included uncorrected distance visual acuity (UDVA), best corrected distance visual acuity (BDVA), manifest and cycloplegic refraction, a full ophthalmic examination including slit-lamp examination, intraocular pressure by Goldmann applanation tonometer measurement, corneal epithelium assessment by fluorescein staining, tear breakup time, and dilated fundus examination. In addition, all patients underwent Scheimpflug tomography (Sirius, CSO, Florence, Italy) and central ultrasound pachymetry.

Surgical technique

All surgeries were performed using the same technique by 2 surgeons (I.S.B. and E.L.), using the SCHWIND Amaris 500E excimer laser platform (SCHWIND eye-tech-solutions GmbH, Kleinostheim, Germany). The OCP platform integrated in the laser system was utilized for the intraoperative central pachymetry measurements. The ablation algorithm was calculated using ORK-CAM software. The target refraction was emmetropia in all eyes.

In the preparation area, one drop of topical 0.4% oxybuprocaine hydrochloride (Localin, Fischer Pharmaceuticals Ltd., Bney Brak, Israel) and moxifloxacin (Vigamox, Alcon Laboratories, Inc., Fort Worth, TX) were instilled in the eyes, and the lid margins were cleaned with a 5% povidone iodine solution. Immediately afterwards, the patient entered the laser room to the supine position of the laser system. A sterile drape was placed at the lid margins and a lid speculum was inserted. The first OCP measurement of the corneal thickness was performed while the tracker was aligned on the eye. Another drop of topical 0.4% oxybuprocaine hydrochloride anesthesia was instilled in the operated eye, and, then, epithelial delamination was achieved with an 8.5-mm well-placed centrally on the cornea and filled with 20% ethanol alcohol for 30 seconds, followed by absorption with a Merocel sponge. The epithelium was debrided using a blunt spatula and the Bowman layer was exposed. A second OCP measurement was obtained prior to the laser ablation. The laser ablation was performed, and Mitomycin C 0.02% was applied to the stromal bed for 10 to 30 seconds depending on the ablation depth. The bed was irrigated with 20 mL of chilled balanced salt solution, and a soft bandage contact lens was placed for four to 6 days. Patients were instructed to use 0.5% moxifloxacin eye drops four times daily for 7 days; 0.5% loteprednol eye drops (Lotemax, Bausch & Lomb, Inc.) four times daily for a month, and then tapered down gradually over the next month; and non-preserved artificial tears as needed.

Data collection

Data were abstracted for age, gender, contact lens wear, refractive errors, and keratometry (Javal keratometry). Central corneal pachymetry data were obtained as recorded from the ultrasound pachymetry and the tomographic parameter of minimal corneal thickness. Intraoperative data of the OCP measurement were recorded. The central ET was calculated by subtracting OCP measurement after epithelium removal from the OCP measurement prior to epithelium removal.

Statistical analysis

Data were recorded in Microsoft Excel 2010 and analyzed using SPSS version 25 (SPSS Inc., Chicago, IL, USA).

Continuous variables, such as epithelial thickness, were compared between subjects using the independent sample t-test. In cases of paired variables, such as right and left comparison, data were restructured and paired sample t-test was used.

Correlation of continuous variables was examined using the Pearson's correlation.

All tests were 2-tailed, and the threshold for statistical significance was defined as a p-value <0.05.

The study included 140 eyes of 70 consecutive patients. The mean age of the patients was 27.29 ± 6.57 years (range, 19–45), 51.4% were females. Contact lens wear was reported to be used in 80 eyes (57.1%). The preoperative refractive measurements for all eyes included a mean sphere of -3.52 ± 1.85 D (range, -0.25 to -7.5 D); a mean cylinder of -0.67 ± 0.66 D (range, -0.45 to +5.00 D); and a mean spherical equivalent of -3.18 ± 1.84 D (range, -0.37 to +7.5 D). Table 1 demonstrates patients’ baseline characteristics.

Intraoperative corneal thickness measurements were significantly correlated to the ET measurements (p=0.04, r_p=0.3), as well as to the ultrasonic pachymetry and tomography measurements (p<0.001, r_p=0.79 and r_p=0.82, respectively).

The mean epithelium thickness was 59.49 ± 19.93 µm, ranging from 15 to 150 µm (Figure 1). Sixty-four eyes (45.7%) had an ET within the range between 40-60 μm, while in 76 eyes (54.3%) the ET was either below (14 eyes, 10%) or above (62 eyes, 44.3%) these values.

There was no significant difference in ET between males and females (p=0.29) and no association was found to contact lens wear (p=0.11). In addition, ET was not correlated to patient age (p=0.18, r_p=0.13), refractive errors (p>0.11, r_p=-0.02-0.14), nor to keratometry (p>0.64, r_p=-0.01-0.02). The results are summarized in Table 1.

While comparing the first and second operated eyes’ ET for each patient, we found that the ET in the second operated eye was significantly thicker than the first operated eye (p=0.006). Figure 2 illustrates the difference between the second and first operated eye for each patient, showing an increase in thickness of the second eye for the vast majority of the patients.

To our best knowledge, this is the first study to assess the real-life intraoperative central corneal epithelium thickness during alcohol-assisted PRK. Utilizing the OCP integrated in the SCWIND Amaris excimer laser platform, we found an overall high variability of the central corneal epithelium. In addition, the second operated eye had significantly thicker central epithelium than the first operated eye.

Several strategies have been reported in the literature for the measurement of epithelium thickness, including VHF digital ultrasound,[1, 3] optical pachymetry,[7, 17] anterior-segment OCT[4–6, 8–10, 18] and confocal microscopy.[19] The advances in imaging techniques enabled clinical applications of ET mapping. This tool is used in the preoperative evaluation of refractive surgery candidates and may provide data of the individual epithelium profile to diagnose subclinical disorders. However, these maps, obtained preoperatively, do not necessarily reflect intraoperative changes in the epithelium that may affect the ablation impact.

The OCP is used for continuous monitoring of the corneal thickness intraoperatively. OCP measurements have been previously reported to show a high reproducibility.[20–22] These reports are in agreement with our findings, in which the initial intraoperative OCP measurement of corneal thickness was significantly correlated to all preoperative corneal thickness measurements, both by US pachymetry and topography. Moreover, all measurements of corneal thickness in our study showed no difference between fellow eyes.

The calculated mean central ET in our study was 59.5 ± 19.9, similar to previous studies that reported a mean ET between 48 ± 5 μm[17] and 59.9 ± 5.9 μm.[7] However, in our study the high standard deviation (19.9 µm), reflecting the wide range of ET between 15-150 µm, supports the need for caution when referring intraoperatively to preoperative measurements, which may not reflect the intraoperative changes.

Our results did not show a difference in ET between genders and CL wear, nor did we find a correlation with age, refractive errors, or keratometry. The literature is controversial about whether age, gender, or refractive errors affect the corneal ET.[4–7] Several studies[8, 14, 17] showed a significant decrease in ET among CL-wear groups, with and an increase in thickness following discontinuation of the soft contact lenses.[8] It is important to note that our group of patients, seeking refractive surgery, were mostly young adults with varying degrees of myopia. All long-term CL wearers were requested to cease using CL at least 10 days prior to surgery. It may be partially for these reasons that we saw no age or CL-related ET differences, even though such differences have been observed in other publications.

When comparing consecutive eyes for each patient, we found that the corneal epithelium of the second operated eye was significantly thicker than that of the first operated eye (54.8 ± 17.6 compared to 64.1 ± 21.1, p=0.006). Studies assessing the preoperative epithelium, using VHF digital ultrasound[3] and OCT,[14, 23] showed no difference in epithelium thickness between the right and left eyes. It is possible that our findings can be explained by the time elapsed between the first and second operated eye.

Another factor that can affect the ET is the topical anesthesia. Several studies reported that the topical anesthetic agents proparacaine 0.5%[24–26] and oxybuprocaine 0.4%[24, 27] can cause a small transient increase in central corneal thickness. Mukhopadhyay et al.[26] found a large degree of interindividual variability in the amount of swelling, ranging from a decrease of more than 10 µm to an increase of over 30 µm in individual cases. Asensio et al.[27] reported a similar degree of variability, and concluded that topical anesthesia can affect corneal thickness by more than 10 µm in over 25% of the patients. Weekers et al.[28] revealed that cocaine, lidocaine, and benoxinate cause an alteration of the Na+/K+ endothelium pump on the corneal epithelium in rabbits, resulting in increased osmotic pressure and subsequent increased hydration of the epithelium and stroma. In our study, a drop of 0.4% oxybuprocaine was instilled in both eyes at the beginning of the surgery. The toxic effect of the anesthetic was probably greater in the second operated eye, in which more time elapsed between the installation of the anesthetic drops and the epithelial removal. This may support the high variability of ET and the difference between the first and the second operated eye.

Our study has several limitations. First, the values of ET were assessed indirectly by subtracting OCP measurements before and after epithelium removal. Second, although the toxic effect of the topical anesthesia could have also affected the stroma, affecting the epithelium calculation, our measurements of the total corneal thickness did not differ from the preoperative measurements.

Transepithelial PRK (SCHWIND eye-tech-solutions GmbH, Kleinostheim, Germany) is becoming a popular refractive procedure.[29–31] A single step standard epithelial ablation pattern of 55 μm centrally is performed followed immediately by the stromal ablation. Although many researchers showed the efficacy and safety of t-PRK,[29–31] concerns were raised about possible effect of interindividual epithelial thickness profile variability on the refractive outcomes.[32, 33] A recent study by Jun et al.[34] showed a significant difference in postoperative sphere and spherical equivalent between groups of ET undergoing t-PRK, with a mild hyperopic shift in the group of ET < 50 μm, and a slight myopic shift in the 60 μm or greater ET group. This phenomenon can be explained by less stromal ablation at the same ablation depth setting. Our intraoperative cohort showed that 45.7% of the eyes had an ET within the range between 40-60 μm, while 54.3% were below or above these values. The variation of ET found in our study can be similarly applicable to t-PRK and support the need of a real-life assessment of the ET and addressing the measurements in the excimer laser ablation profile.

In conclusion, the intraoperative OCP measurements during alcohol-assisted PRK showed a high variability of central corneal epithelium thickness, with the second operated eye being significantly thicker than the first operated eye. The intraoperative measurements may differ from the preoperative evaluation and may have potential implications (under- or overcorrections) on the refractive outcomes of surface keratorefractive surgery when not included in the surgical planning. Additional studies correlating between the preoperative epithelial profile and the real-life intraoperative measurements are warranted.

Ethics approval and consent to participate: The study was approved by the Institutional Review Board at the Sheba Medical Center and complied with the Declaration of Helsinki.

Consent for publication: Not applicable

Availability of data and materials: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Competing interests: The authors declare that they have no competing interests.

Funding: Not applicable

Authors' contributions: DB made the acquisition of data and was a major contributor in writing the manuscript. EL made substantial contributions to the conception of the study. AR analyzed and interpreted the patient data. SL made substantial contributions to the conception of the study. IB made substantial contributions to the conception and was a major contributor in writing the manuscript. All authors read and approved the final manuscript.

Acknowledgments: The authors thank Arik Gendlin and Efrat Harel, from Enaim Refractive Surgery Center, for their technical assistance with precuring the data.

Reinstein DZ, Silverman RH, Trokel SL, Coleman DJ. Corneal Pachymetric Topography. Ophthalmology. 1994;101:432–8. doi:10.1016/S0161-6420(94)31314-5.
Simon G, Ren Q, Kervick GN, Parel JM. Optics of the corneal epithelium. Refract Corneal Surg. 1993;9:42–50.
Reinstein DZ, Archer TJ, Gobbe M, Silverman RH, Coleman DJ. Epithelial thickness in the normal cornea: three-dimensional display with very high frequency ultrasound. J Refract Surg. 2008;24:571–81. doi:10.1016/j.bbi.2008.05.010.
Samy MM, Shaaban YM, Badran TAF. Age- and sex-related differences in corneal epithelial thickness measured with spectral domain anterior segment optical coherence tomography among Egyptians. Med (United States). 2017;96:1–7.
Jun B, Ryu KI, Woong S. Age-related differences in corneal epithelial thickness measurements with anterior segment optical coherence tomography. Jpn J Ophthalmol. 2016;60:357–64.
Kim BJ, Ryu IH, Lee JH, Kim SW. Correlation of sex and myopia with corneal epithelial and stromal thicknesses. Cornea. 2016;35:1078–83.
Wang X, Dong J, Wu Q. Corneal thickness, epithelial thickness and axial length differences in normal and high myopia. BMC Ophthalmol. 2015;15:1–5.
Lei Y, Zheng X, Hou J, Xu B, Mu G. Effects of long-term soft contact lens wear on the corneal thickness and corneal epithelial thickness of myopic subjects. Mol Med Rep. 2015;11:2020–6.
Cui X, Hong J, Wang F, Deng SX, Yang Y, Zhu X, et al. Assessment of Corneal Epithelial Thickness in Dry Eye Patients. Optom Vis Sci. 2014;91:1446–54.
Kanellopoulos AJ, Asimellis G. In Vivo 3-Dimensional Corneal Epithelial Thickness Mapping as an Indicator of Dry Eye: Preliminary Clinical Assessment. Am J Ophthalmol. 2019;157:63-68.e2. doi:10.1016/j.ajo.2013.08.025.
Serrao S, Lombardo G, Calì C, Lombardo M. Role of corneal epithelial thickness mapping in the evaluation of keratoconus. Contact Lens Anterior Eye. 2019; February:0–1. doi:10.1016/j.clae.2019.04.019.
Eckard A, Stave J, Guthoff RF. In vivo investigations of the corneal epithelium with the confocal Rostock Laser Scanning Microscope (RLSM). Cornea. 2006;25:127–31. doi:10.1097/01.ico.0000170694.90455.f7.
Li HF, Petroll WM, Møller-Pedersen T, Maurer JK, Cavanagh HD, Jester J V. Epithelial and corneal thickness measurements by in vivo confocal microscopy through focusing (CMTF). Curr Eye Res. 1997;16:214–21. http://www.ncbi.nlm.nih.gov/pubmed/9088737. Accessed 25 May 2019.
Haque S, Simpson T, Jones L. Corneal and epithelial thickness in keratoconus: a comparison of ultrasonic pachymetry, Orbscan II, and optical coherence tomography. J Refract Surg. 2006;22:486–93. http://www.ncbi.nlm.nih.gov/pubmed/16722488. Accessed 25 May 2019.
Li Y, Tan O, Brass R, Weiss JL, Huang D. Corneal epithelial thickness mapping by Fourier-domain optical coherence tomography in normal and keratoconic eyes. Ophthalmology. 2012;119:2425–33. doi:10.1016/j.ophtha.2012.06.023.
Wirbelauer C, Pham DT. Continuous monitoring of corneal thickness changes during LASIK with online optical coherence pachymetry. J Cataract Refract Surg. 2004;30:2559–68. doi:10.1016/j.jcrs.2004.04.068.
Pérez JG, Méijome JMG, Jalbert I, Sweeney DF, Erickson P. Corneal epithelial thinning profile induced by long-term wear of hydrogel lenses. Cornea. 2003;22:304–7.
Wasielica-Poslednik J, Lisch W, Bell K, Weyer V, Pfeiffer N, Gericke A. Reproducibility and daytime-dependent changes of corneal epithelial thickness and whole corneal thickness measured with fourier domain optical coherence tomography. Cornea. 2016;35:342–9.
Patel S V., McLaren JW, Hodge DO, Bourne WM. Confocal microscopy in vivo in corneas of long-term contact lens wearers. Investig Ophthalmol Vis Sci. 2002;43:995–1003.
Wirbelauer C, Aurich H, Pham DT. Online optical coherence pachymetry to evaluate intraoperative ablation parameters in LASIK. Graefe’s Arch Clin Exp Ophthalmol. 2007;245:775–81.
Siebelmann S, Horstmann J, Scholz P, Bachmann B, Matthaei M, Hermann M, et al. Intraoperative changes in corneal structure during excimer laser phototherapeutic keratectomy (PTK) assessed by intraoperative optical coherence tomography. Graefe’s Arch Clin Exp Ophthalmol. 2018;256:575–81.
Adib-Moghaddam S, Arba-Mosquera S, Salmanian B, Omidvari AH, Noorizadeh F. On-line pachymetry outcome of ablation in aberration free mode transPRK. Eur J Ophthalmol. 2014;24:483–9.
Feng Y, Simpson TL. Comparison of human central cornea and limbus in vivo using optical coherence tomography. Optom Vis Sci. 2005;82:416–9. http://www.ncbi.nlm.nih.gov/pubmed/15894917. Accessed 28 Jul 2019.
Nam SM, Lee HK, Kim EK, Seo KY. Comparison of corneal thickness after the instillation of topical anesthetics: Proparacaine versus oxybuprocaine. Cornea. 2006;25:51–4.
Herse P, Siu A. Short-term effects of proparacaine on human corneal thickness. Acta Ophthalmol. 1992;70:740–4. http://www.ncbi.nlm.nih.gov/pubmed/1488880. Accessed 28 Jul 2019.
Mukhopadhyay DR, North R V, Hamilton-Maxwell KE. Effect of a proparacaine 0.50%-sodium fluorescein 0.25% mix and contact ultrasound pachymetry on central and midperipheral corneal thickness measured by noncontact optical pachymetry. J Cataract Refract Surg. 2011;37:907–13. doi:10.1016/j.jcrs.2010.11.033.
Asensio I, Rahhal SM, Alonso L, Palanca-Sanfrancisco JM, Sanchis-Gimeno JA. Corneal thickness values before and after oxybuprocaine 0.4% eye drops. Cornea. 2003;22:527–32. http://www.ncbi.nlm.nih.gov/pubmed/12883345. Accessed 28 Jul 2019.
Weekers JF. [Experimental studies of the genesis of corneal lesions caused by anesthetics]. Arch Ophtalmol Rev Gen Ophtalmol. 1974;34:121–32. http://www.ncbi.nlm.nih.gov/pubmed/4277188. Accessed 28 Jul 2019.
Aslanides IM, Padroni S, Arba Mosquera S, Ioannides A, Mukherjee A. Comparison of single-step reverse transepithelial all-surface laser ablation (ASLA) to alcohol-assisted photorefractive keratectomy. Clin Ophthalmol. 2012;6:973–80. doi:10.2147/OPTH.S32374.
Luger MHA, Ewering T, Arba-Mosquera S. Consecutive myopia correction with transepithelial versus alcohol-assisted photorefractive keratectomy in contralateral eyes: one-year results. J Cataract Refract Surg. 2012;38:1414–23. doi:10.1016/j.jcrs.2012.03.028.
Fadlallah A, Fahed D, Khalil K, Dunia I, Menassa J, El Rami H, et al. Transepithelial photorefractive keratectomy: clinical results. J Cataract Refract Surg. 2011;37:1852–7. doi:10.1016/j.jcrs.2011.04.029.
Salah-Mabed I, Saad A, Gatinel D. Topography of the corneal epithelium and Bowman layer in low to moderately myopic eyes. J Cataract Refract Surg. 2016;42:1190–7. doi:10.1016/j.jcrs.2016.05.009.
Mosquera SA, Awwad ST. Theoretical analyses of the refractive implications of transepithelial PRK ablations. Br J Ophthalmol. 2013;97:905–11.
Jun I, Yong Kang DS, Arba-Mosquera S, Kim EK, Seo KY, Kim TI. Clinical outcomes of transepithelial photorefractive keratectomy according to epithelial thickness. J Refract Surg. 2018;34:533–40.

TABLE 1. Association between epithelium thickness to demographics and preoperative measurements

r_p	p		Eyes n=140	Patients n=70 n=20

0.13	0.18			27.29 ± 6.57	Age^a, years (mean ± SD)
	0.29			36 (51.4)	Gender-female, n (%)
	0.11		80 (57.1)		History of contact lens, n (%)
					Refractive errors, D (mean ± SD)
0.16	0.11		-3.52 ± 1.85		Sphere
0.05	0.60		-0.67 ± 0.66		Cylinder
0.15	0.13		-3.18 ± 1.84		Spherical equivalent
					Keratometry, D (mean ± SD)
0.02	0.82		43.22 ± 1.59		K1
-0.05	0.64		44.18 ± 1.59		K2
-0.01	0.89		43.73 ± 1.55		K average
					Preoperative pachymetry, µm (mean ± SD)
0.06	0.57		538.03 ± 27.96		US pachymetry
0.09	0.37		540.5 ± 27.9		Topography (Sirius)
0.30	0.04		537.57 ± 31.71		Optical coherence pachymetry
		US, ultrasound. ^aAge: Age at first examination.

Real-Life Variability of Corneal Epithelium Thickness in Photorefractive Keratectomy

Status:

Version 1

Abstract

Figures

Introduction

Materials and Methods

Results

Discussion

Declarations

References

Tables

Status:

Version 1