A total of 108 women aged 12-18 years old participated in the study, including 53 patients with AN (R-AN=30, B-AN=23) and 55 age-matched healthy controls (HCs). The patient group consisted of 30 patients with R-AN and 23 patients with B-AN.
Comparison of general information
There was no significant difference in age among the three groups; BMI, T, P, DBP and SBP showed significant differences (P<0.01). There was no significant difference in disease course or amenorrhea time between the R-AN and B-AN groups; the disease course was longer than 9 months, and the time of amenorrhea was more than 4 months. T, P, DBP and SBP showed significant differences between the R-AN group and the control group. The same results existed between the R-AN and B-AN groups (P<0.01), while DBP was significantly different between the B-AN group and control group (P<0.01) (Table 1).
Comparison of laboratory data between three groups
There were significant differences in the three groups with regard to WBC, PRL TT4, TSH FT3, FT4 TT3, TP, HGB, RBC and GLB (P<0.01). The same was true for the R-AN and control groups. There was a significant difference between the B-AN and control groups in WBC, PRL, TT4, FT3, FT4, TT3, TP, HGB, RBC, GLB and PLT (P<0.01). There were significant differences in PRL and TSH (P<0.01) and FT4 and HGB (P<0.05) between the two groups. (Table 1).
Table 1:Demographic characteristics and clinical data (Mean ± SD) of patients with anorexia nervosa and healthy controls
|
R-AN(n=30)
|
B-AN(n=23)
|
HC(n=55)
|
P
|
PR-H
|
PB-H
|
PR-B
|
Age (mean±SD)
|
14.53±0.243
|
15.04±0.395
|
15.00±0.217
|
0.371
|
0.159
|
0.916
|
0.357
|
BMI
|
13.567±0.344
|
15.199±0.471
|
21.287±0.242
|
0.000
|
0.000
|
0.000
|
0.009
|
course of disease
|
9.9±0.827
|
11.09±0.616
|
|
---
|
----
|
---
|
0.170
|
amenorrhea
|
5.717±0.619
|
4.948±0.788
|
|
----
|
---
|
---
|
0.272
|
T
|
35.723±.0985
|
36.230±0.075
|
36.38±.0416
|
0.000
|
0.000
|
0.064
|
0.002
|
HR
|
62.00±1.986
|
70.826±2.262
|
72.109±1.060
|
0.000
|
0.000
|
0.684
|
0.004
|
DBP
|
89.33±2.199
|
97.96±1.973
|
102.96±1.011
|
0.000
|
0.000
|
0.053
|
0.007
|
SBP
|
56.83±1.423
|
63.35±1.489
|
78.76±1.040
|
0.000
|
0.000
|
0.000
|
0.012
|
WBC
|
4.552±0.356
|
4.507±0.293
|
6.1605±.1737
|
0.000
|
0.000
|
0.000
|
0.760
|
PLT
|
235.6±13.459
|
243.09±12.515
|
213.95±6.229
|
0.092
|
0.196
|
0.033
|
0.554
|
HGB
|
101.387±4.150
|
110.696±2.874
|
125.745±1.235
|
0.000
|
0.000
|
0.000
|
0.043
|
RBC
|
3.614±.1107
|
3.823±.1069
|
4.238±0.055
|
0.000
|
0.000
|
0.000
|
0.101
|
PRL
|
26.528±3.878
|
33.657±2.737
|
8.287±0.5087
|
0.000
|
0.000
|
0.000
|
0.006
|
TT4
|
5.594±.5616
|
6.055±0.7140
|
8.173±0.300
|
0.000
|
0.000
|
0.001
|
0.609
|
TSH
|
10.473±1.804
|
4.424±1.526
|
3.318±0.380
|
0.001
|
0.001
|
0.15
|
0.002
|
FT3
|
3.007±1.350
|
2.106±0.169
|
2.855±0.092
|
0.000
|
0.000
|
0.000
|
0.106
|
FT4
|
0.545±0.043
|
0.761±0.106
|
0.860±.0385
|
0.000
|
0.000
|
0.017
|
0.035
|
TT3
|
0.545±0.041
|
0.654±.055
|
1.031±.0340
|
0.000
|
0.000
|
0.000
|
0.167
|
The comparison of EDI scores
EDI scores were significantly different among the three groups, the total score and DT, ID, and IA were significantly different between the R-AN and control groups, and BD, P, and MF were significantly different between the R-AN and control groups (P<0.01). Total scores and DT, BD, ID, and IA were significantly different between the B-AN and control groups (P<0.01), while differences existed in P (P<0.05). There were only significant differences in the total score and B between the case groups (P<0.01). (Table 2).
Correlation of BMI between case groups
In the R-AN group, BMI was negatively correlated with amenorrhea (r=-0.47, p<0.01), PRL(r=-0.44, p<0.05), EDI total score (r=-0.55, p<0.01), DT (r=-0.67, p<0.01), BD (r=-0.70, p<0.01), and P (r=-0.44, p<0.05) and positively correlated with T (r=0.53, p<0.01) DBP(r=0.26, p<0.05), SBP (r=0.51, p<0.01), HGB (r=0.46, p<0.05), and GLB (r=0.39, p<0.05). In the B-AN group, BMI was negatively correlated with amenorrhea (r=-0.57, p<0.01), EDI total score (r=-0.64, p<0.01), DT (r=-0.43, p<0.05), B (r=-0.42, p<0.05), BD (r=-0.44, p<0.05), and IE (r=-0.60, p<0.01) and positively correlated with T (r=0.56, p<0.01) and DBP (r=0.57, p<0.01). (Table 3).
Table 2: EDI total score and each factor score in anorexia nervosa patients and healthy controls
|
R-AN
|
B-AN
|
HC
|
P
|
PR-H
|
PB-H
|
PR-B
|
TOTA
|
183.97±2.457
|
196.3±3.177
|
137.96±1.252
|
0.000
|
0.000
|
0.000
|
0.004
|
DT
|
28.47±0.851
|
26.04±1.199
|
12.95±0.491
|
0.000
|
0.000
|
0.000
|
0.189
|
B
|
9.3±0.304
|
22.22±0.896
|
9.69±0.276
|
0.001
|
0.396
|
0.000
|
0.000
|
BD
|
22.7±0.842
|
23.65±0.951
|
20.22±0.492
|
0.003
|
0.028
|
0.002
|
0.45
|
IE
|
25.2±0.914
|
25.57±1.09
|
20.31±0.504
|
0.000
|
0.000
|
0.000
|
0.801
|
P
|
22.47±0.853
|
22.7±1.055
|
19.2±0.75
|
0.003
|
0.011
|
0.022
|
0.942
|
ID
|
21.1±0.961
|
21.52±1.332
|
13.11±0.432
|
0.000
|
0.000
|
0.000
|
0.801
|
IA
|
28.3±0.966
|
28.7±1.438
|
17.96±0.528
|
0.000
|
0.000
|
0.000
|
0.950
|
MF
|
26.7±0.618
|
26.35±.0919
|
24.53±0.545
|
0.002
|
0.011
|
0.103
|
0.679
|
Table 3: The correlation between clinical characteristics, EDI score, all factors score and BMI in case group
|
R-AN
|
|
B-AN
|
r
|
P
|
r
|
P
|
amenorrhea
|
-0.474
|
0.008
|
|
-.569
|
.005
|
T
|
0.528
|
0.003
|
|
.557
|
.005
|
P
|
0.267
|
0.154
|
|
.223
|
.305
|
DBP
|
0.263
|
0.012
|
|
.567
|
.005
|
SBP
|
0.513
|
0.004
|
|
.108
|
.623
|
WBC
|
-0.264
|
0.158
|
|
.277
|
.201
|
GLU
|
0.306
|
0.100
|
|
.023
|
.916
|
PLT
|
-0.185
|
0.327
|
|
.143
|
.156
|
HGB
|
0.459
|
0.011
|
|
.209
|
.338
|
PRL
|
-0.443
|
0.014
|
|
.210
|
.336
|
TT4
|
0.261
|
0.163
|
|
.137
|
.532
|
TSH
|
0.098
|
0.608
|
|
.195
|
.373
|
FT3
|
0.145
|
0.445
|
|
.205
|
.349
|
TT3
|
0.316
|
0.089
|
|
.195
|
.372
|
EDI:
TOTA
|
-0.554
|
0.000
|
|
-0.644
|
0.001
|
DT
|
-0.668
|
0.000
|
|
-0.432
|
0.04
|
B
|
0.021
|
0.913
|
|
-0.416
|
0.048
|
BD
|
-0.698
|
0.000
|
|
-0.437
|
0.037
|
IE
|
0.108
|
0.568
|
|
-0.598
|
0.003
|
P
|
-0.438
|
0.016
|
|
-0.222
|
0.309
|
ID
|
-0.204
|
0.28
|
|
0.083
|
0.707
|
IA
|
0.136
|
0.474
|
|
-0.245
|
0.259
|
MF
|
0.214
|
0256
|
|
0.261
|
0.229
|