Setting clear biodiversity targets is a pervasive challenge1 due to the context-dependent nature of biodiversity that has evaded concise science-based objectives such as the 1.5°C for climate2. Considering the major risk of continued inaction, and further biodiversity loss, it is imperative that the biodiversity community identify similarly operational science-based boundaries supporting the development of targets to safeguard biodiversity and its contributions to human well-being. Confounding goal setting are the two distinct but not mutually exclusive conservation objectives: (1) halting the rampant loss of intact ecosystems, species extinction and population declines, and (2) maintaining biosphere integrity and ecosystem function. We propose a revised planetary boundary for biodiversity: retaining at least half of the area of each terrestrial ecoregion biologically intact to halt the extinction crisis, and maintaining ecosystem integrity across all lands to preserve and regenerate biosphere, ecosystem functions and their contributions to human well-being. We combine four intactness datasets to provide a global assessment and find that 49.6% of the Earth’s land ice-free surface remains intact. While this is promising globally, 69% of 798 unique ecoregions are less than half intact. For ecosystem integrity, we find 18.1% of working lands have ecosystem integrity deficits precluding the provisioning of biosphere and ecosystem functions. Ninety percent of ecoregions have an ecosystem integrity deficit. Globally, intactness and integrity are at boundary limits with degradation of nature critically jeopardizing biosphere capacity to support a safe and just space for humanity. Combined efforts are needed to halt loss of and restore intactness, while regenerating integrity in working lands.