Topological spin textures are characterized by topological magnetic charges Q, which govern their fascinating electro-magneto properties. Recent studies have achieved skyrmion bundles with arbitrary integer values of Q, opening up possibilities for exploring topological spintronics based on the parameter freedom of Q. However, the realization of stable skyrmion bundles in chiral magnets at room temperature and zero magnetic field, which is the prerequisite for realistic device applications, has remained exclusive. Here, through the combination of pulsed currents and reversed magnetic fields, we experimentally achieved skyrmion bundles with different inter-Q values, reaching a maximum of 24 at above room temperature and zero magnetic field in a β-Mn-type Co8Zn10Mn2 chiral magnet. We demonstrate the field-driven topological quantitated annihilation of high-Q bundles and present a stable phase diagram as a function of temperature and field. Our experimental findings are consistently corroborated by micromagnetic simulations. The observation of skyrmion bundles and their multi-Q topological properties at room temperature and zero fields could promote realistic multi-Q-based topological spintronic devices.