The preservation of the region's biodiversity is seriously threatened by the area's growing urbanization. Because of the abundance of biodiversity in Xishuangbanna Prefecture, it is crucial to create wilderness networks that link scattered wilderness areas and enhance network connection to preserve wilderness areas and ecosystem stability. Boolean overlay and Weighted Linear Combination (WLC), two methods for integrating wilderness mapping, were used in this work to identify the wilderness patches and wilderness maps. Morphological spatial pattern analysis (MSPA) and connectivity analysis were used to locate the wilderness source and structural corridor, respectively. The wilderness source was then extracted using the Minimal Cumulative Resistance Mode (MCR) model and the Linkage Mapper potential corridor tool. Construct the wilderness network by superimposing each indicator, and optimize the wilderness network by adding wilderness sources, building footstones, and adding wilderness corridors. Results show that the constructed wilderness network included 57.37hm2 of structural corridors, 15 wilderness source areas, 31 wilderness potential corridors, and 27 ecological pinch points. The wilderness network was optimized by adding 4 wilderness source sites, 9 stepping stones, and 6 wilderness addition corridors, the optimized wilderness network connectivity was improved, with the α, β, and γ values increasing by 0.16, 0.29, and 0.11, respectively. To better protect areas of high wilderness value in the biodiversity tropics along South-west China's border and enhance the nature reserve system, this study analyses the wilderness network's connectivity based on naturalness and connectivity. It also offers reference data for the development of policies such as territorial spatial planning, the delineation of the ecological protection red line, and the ecological protection and restoration of mountains, waters, forests, lakes, and grasslands.