Toxicity due to the presence of heavy metals in ground and surface water due to the release of effluents from industries holds the potency to cause temporary or permanent damage to the living organisms in the near vicinity of these industries. This paper aims to assess the vulnerability of groundwater resources owing to its contamination in Banthar Industrial Area. Using DRASTIC model in combination with Quantum Geographic Information System (QGIS), a groundwater vulnerability map was developed, which indicated that the dispersal of Chromium was in the range of 300–1000 µg/l. In order to study the impact of Chromium dispersion, the authors proposed a batch adsorption testing of the Hexavalent Chromium (Cr (VI)) using different bio-adsorbent materials, keeping a pH range of 3–9 and the contact time between 0–80 minutes. Adsorption isotherms and Kinetic studies were also considered. All adsorbents followed the Langmuir isotherm (R2 > 0.986), whereas Temkin isotherm stated the affinity of the adsorbents for the Cr (VI). The reactions between Chromium and the bio-adsorbents were found to follow pseudo-second order kinetics, which indicated that the reactions were exothermic. The results proposed that a modification in the bio-adsorbents caused an enhancement in the percent adsorption of Cr (VI) in comparison to the natural available bio-adsorbents.