Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging

doi:10.21203/rs.3.rs-3948627/v1

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Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging

https://doi.org/10.21203/rs.3.rs-3948627/v1

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We introduce a super-photostable and bright organic dye, ‘Phoenix Fluor 555 (PF555)’, which exhibits an order-of-magnitude longer photobleaching lifetime than conventional organic dyes without the requirement of any anti-photobleaching additives. PF555 is an asymmetric cyanine structure where one side of indole from the conventional Cy3 is substituted with 3-oxo-quinoline. PF555 provides a powerful tool for long-term live-cell single-molecule imaging, as we demonstrated the dynamic interactions of the single-molecule epidermal growth factor receptor with clathrin-coated structures on the plasma membrane of a live cell under a physiological condition.

Biological sciences/Biological techniques/Sensors and probes/Fluorescent dyes

Biological sciences/Biological techniques/Imaging/Fluorescence imaging

Biological sciences/Biological techniques/Imaging/Molecular imaging

Biological sciences/Biological techniques/Microscopy/Super-resolution microscopy

There is NO Competing Interest.

SuppMov1.mp4
Supplementary Video 1. The raw movie for PF555-labeled EGFR in a live cell
SuppMov2.mp4
Supplementary Video 2. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3d
SuppMov3.mp4
Supplementary Video 3. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3e
SuppMov4.mp4
Supplementary Video 4. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3f
SuppMov5.mp4
Supplementary Video 5. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3g
SuppMov6.mp4
Supplementary Video 6. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3h
SuppMov7.mp4
Supplementary Video 7. The raw movie and single particle tracking of CA-PF555 labeled HALO-EGFR in a live cell for figure 3i
SuppMov8.mp4
Supplementary Video 8. The raw movie and the quantitative analysis for simultaneous multicolor imaging of PF555-labeled EGFR and EGFP-CLC in a live cell

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Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging

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