In order to achieve quantitative analysis of rockburst risk in coal mine, multi source monitoring information of micro-earthquakes needs to be deeply mined. In accordance with the microseismic data collected from the 1366 workface of the Hengda Coal Mine, precise source location, source parameters inversion, and regional stress determination of the workface are carried out. The accurate location technology is proposed, the evolution law of the source parameters are determined, and the regions of stress concentration are determined based on the seismic wave CT technology. Based on this, the reasonable and effective measures of stress relief are put forward. The results show that: (1) The precise source location of micro-earthquakes in the workface is determined based on the combined location method of simplex and double difference; (2) Based on the exact source location, microseismic source parameters such as energy, stress drop, apparent stress, and mean slip are retrieved, and to determine the source properties of a microseismic event, such as energy size and stress state, the evolution laws of the source parameters are analyzed; (3) Based on the seismic wave CT, the stress distribution in different regions was determined, and the regions of stress concentration were defined; (4) According to the microseismic frequency, energy and stress distribution, the dangerous area of the workface is accurately judged, and the reasonable and effective measures of stress relief are proposed for the prevention of rock bursts. Based on the in-depth analysis of multi-source monitoring information of micro-earthquakes in 1366 workface of Hengda Coal Mine, the risk of rockburst was effectively and quantitatively assessed and the safety of coal mine operation process is ensured.