Abstract: Ecosystem service value (ESV) is crucial for understanding regional ecological benefits and resources. This study, based on data from the Chinese Academy of Sciences, examines land use changes in Xi'an over the past two decades. We refined ESV coefficients using a method integrating the biomass factor of Shaanxi Province's farmland ecosystems.Our findings show stable proportions of cultivated and forest land in Xi'an from 2000 to 2020, despite an increase in construction land and a decrease in cultivated areas. Forest and unused lands remained stable, while grassland and water bodies fluctuated. ESV related to land use in Xi'an increased by 938.8 million yuan over this period, with high-value areas mainly in the forested regions south of the Qinling Mountains and along the Weihe, Bahe, and Chanhe Rivers. Low ESV zones were concentrated in the urban core.This research improves methodologies for quantifying urban ESV, providing crucial support for land resource management, ecological conservation, and high-quality urban development in China's major cities. These findings inform policy-making for sustainable urban growth.