Microbiological air sampling was performed at six different Sewage Treatment Plant (STP) locations using a six-stage viable cascade impactor to determine the viable mesophilic gram positive and gram-negative bacterial cell count during all seasons, namely the monsoon, post-monsoon, winter, and pre-monsoon. The bioaerosol concentration of gram-positive bacteria ranged from 12±07 to 997±178 CFU m-3 at different sites of STP with peak values in post-monsoon at the aeration tank (997±178 CFU m-3) and lowest values in winter at the final settlement tank (FST). The minimum concentration of gram-negative bacteria was 35±12 CFU m-3 at inlet 1 during the winter season and maximum with 749±126 CFU m-3 at the aeration tank during the post-monsoon season. Univariate ANOVA analysis showed that sampling vs. seasons and sampler stages had the highest individual effect (p=0.000) and no significant interactive effect (p = 0.611) of seasons and stages on gram positive bacterial bioaerosol concentration at inlet 1. The activated sludge treatment facility was the site where highest individual (p=0.000) and interactive effect (p=0.000) were found for gram negative bacterial bioaerosol. The significant higher regression coefficient (R2) values for gram positive and gram-negative bacteria were observed to be 0.866 and 0.915 respectively in the pre-monsoon season between fine and coarse fractions of aerosols. The maximum concentrations for both types of bacteria were found in the respirable size fraction of particles which are less than 4.7 µm in aerodynamic diameter.