With recent advances in the potential usage of visual gamma stimulation at 40 Hz for the treatment of Alzheimer’s disease, there is motivation to evaluate adjacent frequencies to ensure that specifically 40 Hz is optimal.The choice of 40 Hz is based on studies over several decades that describe "40 Hz" oscillations with considerable frequency variability.As visual stimulation with luminance flicker may affect adherence in clinical trials due to its inherent perceived flickering, invisible spectral flicker (ISF) was proposed as a more comfortable alternative for entraining 40 Hz. This study investigates the ability of ISF to evoke acute gamma responses at several frequencies in the range of 36-44 Hz. Twenty healthy volunteers were included in an electroencephalography (EEG) experiment with an initial eyes-open baseline recording followed by a stimulation paradigm using ISF at nine different frequencies (36-44 Hz, 1 Hz interval). The responses were evaluated as the signal-to-noise ratio (SNR) averaged over the occipital electrodes at the stimulation frequency and analysed using mixed-effect models. Evoked responses were compared to baseline and between frequencies. The estimated SNR values showed the EEG power was significantly increased at all stimulation frequencies compared to baseline, but with no significant difference in SNR between stimulation frequencies. There was a significant subject effect, even when disregarding one subject who responded 1-2 orders of magnitude higher than the others. These results suggest that there is higher variability between subjects than between frequencies alone. Our results indicate that ISF can induce steady-state visually evoked potentials at several frequencies in the low gamma range of 36-44 Hz. Across the population of participants, no preference or trend for any specific gamma stimulation frequency in the tested range was found. While the subject-stimulus interaction was significant, it described little variance and showed no specific patterns for individual preference of frequency.