A study was conducted in freshwater ecosystems in Jasaan, Misamis Oriental, Philippines damselflies (Odonata: Zygoptera) as a biological indicator. Opportunistic sampling was conducted using sweep nets over a span of 15 days. A total of 224 damselfly individuals representing 3 families of the identified 7 species across three sites namely-Solana, San Isidro and San Nicolas were collected and identified. In terms of species richness, Solana yields higher presence of damselfly species and individual abundance, which is followed by San Nicolas and San Isidro. Moreover, San Isidro has the highest diversity index followed by San Nicolas , and Solana with the lowest diversity. In which the species Rhinocypha colorata is the most dominant and abundant species across the sampling areas. Of the three sites, San Nicolas had the highest evenness index indicating a more balanced distribution of individuals compared to the two sites. The study also identified environmental parameter variables among the surveyed locations, highlighting the intricate relationship between altitude and relative humidity influencing damselfly population and species diversity. Overall, the study underscores the urgent need for adaptive conservation strategies in Jasaan, Misamis Oriental, ensuring the long term preservation of biodiversity in these crucial habitat preferences, with San Isidro providing the most favorable conditions for damselfly biodiversity conservation.