Significance tests of straw return methods, potassium fertilization levels and their interactions.
Analysis of variance (ANOVA) results showed that straw return methods and potassium fertilization levels had significant effects on maize photosynthesis, dry matter and yield from 2018 to 2020 (Table 3). Significant interactions between straw return methods and potassium fertilization levels were only found on Pn of 2018 and 2020, and Tr of 2018-2020. Through the comparison of three-year F-values, it could be found that the effect of potassium fertilization levels on maize photosynthesis, dry matter and yield was greater than that of straw return methods.
Table 3. Significance of the effects of straw return methods, potassium fertilization levels and their interactions on maize growth and yield using ANOVA.
Year
|
Source
|
Pn
(μmol·m-2·s-1)
|
Gs
(mmol·m-2·s-1)
|
Tr
(mmol·m-2·s-1)
|
Ci
(μmol·mol-1)
|
Dry matter in
R1 (g/plant)
|
Dry matter in R6(g/plant)
|
Yield
(kg/ha)
|
2018
|
S
|
31.2**
|
24.28**
|
76.15**
|
6.6*
|
9.24**
|
11.01**
|
2.21ns
|
K
|
51.14**
|
67.78**
|
195.24**
|
10.74**
|
20.21**
|
34.07**
|
7.71**
|
S×K
|
5.93**
|
1.09ns
|
9.13**
|
0.34ns
|
0.79ns
|
0.24ns
|
0.07ns
|
2019
|
S
|
12.45**
|
27.71**
|
45.74**
|
4.66*
|
5.55*
|
9.46**
|
4.89*
|
K
|
14.49**
|
114.66**
|
115.35**
|
14.14**
|
13.76**
|
28.22**
|
14.59**
|
S×K
|
2.13ns
|
0.53ns
|
4.17*
|
0.32ns
|
0.54ns
|
0.22ns
|
0.24ns
|
2020
|
S
|
27.55**
|
22.23**
|
38.02**
|
6.91*
|
6.48*
|
13.93**
|
6.29*
|
K
|
40.02**
|
92.37**
|
77.3**
|
22.09**
|
19.06**
|
45.56**
|
16.02**
|
S×K
|
4.03*
|
0.17ns
|
3.27*
|
0.32ns
|
1.23ns
|
0.89ns
|
0.26ns
|
Note: Numbers were F-values. Stars indicated the level of significance (*= p<0.05, **= p<0.01), ns represented insignificant. S represented straw return methods, including SF and FG; K represented potassium fertilization levels, including K0, K30, K45, K60 kg/ha.
Effects of straw return and potassium fertilizer on photosynthesis of maize.
The straw return methods and potassium fertilization levels significantly influenced (p≤0.05) the maize photosynthesis compared to control (CK), resulting in Pn, Gs and Tr values that were higher than those of CK, and Ci value that was lower than that of CK. Straw return and potassium supply increased Pn, Gs and Tr. Compared with CK, under the treatments of SFK0, SFK30, SFK45, SFK60, FGK0, FGK30, FGK45 and FGK60, Pn in 2018 increased by 1.70, 2.65, 5.21, 7.31, 0.63, 2.50, 3.60, 3.97μmol·m-2·s-1; Pn in 2019 increased by 3.12, 4.55, 6.33, 8.85, 2.11, 4.07, 4.68, 5.13μmol·m-2·s-1; and Pn in 2020 increased by 4.09, 5.77, 8.48, 11.44, 3.20, 5.11, 5.79, 7.47 μmol·m-2·s-1 (Fig. 1a). Gs in 2018 increased by 0.06, 0.10, 0.18, 0.20, 0.02, 0.08, 0.13, 0.15 mmol·m-2·s-1; Gs in 2019 increased by 0.07, 0.11, 0.19, 0.22, 0.03, 0.08, 0.17, 0.17 mmol·m-2·s-1; and Gs in 2020 increased by 0.09, 0.13, 0.19, 0.21, 0.06, 0.09, 0.17, 0.19 mmol·m-2·s-1 (Fig. 1b). Tr in 2018 increased by 0.55, 1.02, 1.51, 1.74, 0.49, 0.86, 1.12, 1.27 mmol·m-2·s-1; Tr in 2019 increased by 0.71, 1.24, 1.61, 1.92, 0.61, 1.01, 1.34, 1.41 mmol·m-2·s-1; and Tr in 2020 increased by 0.87, 1.30, 1.67, 1.99, 0.71, 1.13, 1.38, 1.47 mmol·m-2·s-1 (Fig. 1c).
Straw return and potassium supply decreased Ci. Compared with CK, under the treatments of SFK0, SFK30, SFK45, SFK60, FGK0, FGK30, FGK45 and FGK60, Ci in 2018 decreased by 0.55, 1.02, 1.51, 1.74, 0.49, 0.86, 1.12, 1.27μmol·mol-1; Ci in 2019 decreased by 0.71, 1.24, 1.61, 1.92, 0.61, 1.01, 1.34, 1.41μmol·mol-1; and Ci in 2020 decreased by 0.87, 1.30, 1.67, 1.99, 0.71, 1.13, 1.38, 1.47μmol·mol-1 (Fig. 1d).
Comprehensive analysis showed that Pn, Gs, Tr increased and Ci decreased significantly after the treatment of SF under the same potassium supply. Under the same straw return method, Pn, Gs and Tr values increased significantly with the potassium fertilization levels, while Ci decreased. The effects of straw return and potassium fertilizer on maize photosynthesis increased gradually from year to year.
Effects of straw return and potassium fertilizer on dry matter of maize.
We can see from Fig 2, the straw return methods and potassium fertilization levels significantly influenced (p≤0.05) the maize dry matter accumulation compared to CK, resulting in the dry matter values that were higher than that of CK. Straw return and potassium supply increased dry matter accumulation. Compared with CK, under the treatments of SFK0, SFK30, SFK45, SFK60, FGK0, FGK30, FGK45 and FGK60, the dry matter of R1 and R6 stage increased by 17.63, 27.74, 48.28, 60.13, 12.64, 21.15, 35.04, 41.31 and 26.09, 53.74, 81.54, 97.59, 14.49, 40.46, 62.08, 75.73 g/plant in 2018; the dry matter increased by 21.97, 35.87, 52.97, 68.07, 18.73, 27.13, 41.47, 48.83 and 31.38, 64.48, 90.92, 108.03, 20.00, 46.45, 73.53, 83.20 g/plant in 2019; the dry matter increased by 30.73, 43.79, 67.20, 81.46, 29.99, 34.28, 57.57, 57.81 and 39.21, 70.29, 103.97, 122.08, 30.49, 53.17, 87.96, 91.36 g/plant in 2020.
In short, under the same straw return method, the increase of maize dry matter from R1 to R6 improved significantly with the potassium level, potassium fertilizer could improve the maize dry matter accumulation ability. The maize dry matter of R1 to R6 increased significantly after the treatment of SF compared to FG under the same potassium supply. The promotion effect of straw return and potassium fertilizer on maize dry matter increased from year to year.
Effects of straw return and potassium fertilizer on maize yield.
The straw return methods and potassium fertilization levels significantly influenced (p≤0.05) the maize yield compared to CK, resulting in maize yield values that were higher than those of CK. Straw return and potassium supply increased maize yield. Compared to CK, under the treatments of SFK0, SFK30, SFK45, SFK60, FGK0, FGK30, FGK45 and FGK60, maize yield in 2018 increased by 9.73%, 15.74%, 24.02%, 24.46%, 5.84%, 13.51%, 18.64%, 21.19% (Fig. 3a); maize yield in 2019 increased by 9.79%, 15.68%, 25.44%, 25.47%, 5.79%, 13.67%, 18.86%, 21.29% (Fig. 3b); and maize yield in 2020 increased by 10.32%, 17.47%, 25.58%, 25.76%, 7.83%, 13.72%, 19.01%, 21.69% (Fig. 3c).
The maize yield among treatments was as follows: SFK60>SFK45>FGK60>FGK45> SFK30>FGK30>SFK0>FGK0>CK. Compared to FG, the effect of SF on maize yield was more obvious. The maize yield increased significantly with the potassium fertilization levels under the potassium fertilization levels of 0-60kg/ha in this test. The treatment of SFK60 recorded the highest average yield in the three-year test, which was 14744.39kg/ha. The maize yield in different planting years showed as follows: 2020>2019>2018, which indicated that the promotion effect of straw return and potassium fertilizer on maize yield increased from year to year.
Correlation analysis of photosynthesis, dry matter accumulation and yield of maize.
Pn, Gs, Tr and Ci were significantly correlated with dry matter accumulation. Pn, Gs and Tr were positively correlated with dry matter, while Ci was negatively correlated with dry matter (Table 4). The results showed that the increase of Pn, Gs, Tr and the decrease of Ci could significantly improve maize dry matter. Dry matter was positively correlated with maize yield, indicating that the increase of dry matter accumulation could significantly improve maize yield. The increase of Pn, Gs, Tr and dry matter accumulation,as well as the decrease of Ci could significantly increase maize yield.
Under the method of SF, the correlation coefficients of Pn, Gs, Tr, dry matter at R1 stage, dry matter at R6 stage and Ci with yield were 0.862, 0.988, 0.962, 0.948, 0.971 and -0.978;the correlation coefficients were 0.838,0.975,0.970,0.930,0.979 and -0.973 under the method of FG. The results showed that, under the method of SF, the correlation coefficients between dry matter of R1 stage, Pn, Gs, Ci with yield were higher than that under the method of FG, which indicated that SF could promote the correlation between dry matter of R1 stage, Pn, Gs, Ci with yield. Under the method of FG, the correlation coefficients between dry matter of R6 stage, Tr with yield were higher than that under the method of SF, which indicated that FG could promote the correlation between dry matter of R6 stage, Tr with yield.
Table 4. Correlation analysis of photosynthesis, dry matter accumulation and yield of maize under two straw return methods.
Method
|
Index
|
Pn (μmol·m-2·s-1)
|
Gs (mmol·m-2·s-1)
|
Tr (mmol·m-2·s-1)
|
Ci (μmol·mol-1)
|
Dry matter in R1(g/plant)
|
Dry matter in R6 (g/plant)
|
Yield (kg/ha)
|
SF
|
Pn (μmol·m-2·s-1)
|
1
|
|
|
|
|
|
|
Gs (mmol·m-2·s-1)
|
0.900**
|
1
|
|
|
|
|
|
Tr (mmol·m-2·s-1)
|
0.939**
|
0.982**
|
1
|
|
|
|
|
Ci (μmol·mol-1)
|
-0.933**
|
-0.995**
|
-0.989**
|
1
|
|
|
|
Dry matter in R1 (g/plant)
|
0.965**
|
0.971**
|
0.978**
|
-0.981**
|
1
|
|
|
Dry matter in R6 (g/plant)
|
0.945**
|
0.980**
|
0.992**
|
-0.986**
|
0.989**
|
1
|
|
Yield (kg/ha)
|
0.862**
|
0.988**
|
0.962**
|
-0.978**
|
0.948**
|
0.971**
|
1
|
FG
|
Pn (μmol·m-2·s-1)
|
1
|
|
|
|
|
|
|
Gs (mmol·m-2·s-1)
|
0.873**
|
1
|
|
|
|
|
|
Tr (mmol·m-2·s-1)
|
0.923**
|
0.978**
|
1
|
|
|
|
|
Ci (μmol·mol-1)
|
-0.912**
|
-0.970**
|
-0.986**
|
1
|
|
|
|
Dry matter in R1 (g/plant)
|
0.881**
|
0.971**
|
0.948**
|
-0.948**
|
1
|
|
|
Dry matter in R6 (g/plant)
|
0.887**
|
0.989**
|
0.984**
|
-0.978**
|
0.981**
|
1
|
|
Yield (kg/ha)
|
0.838**
|
0.975**
|
0.970**
|
-0.973**
|
0.930**
|
0.979**
|
1
|
Effects of straw return and potassium fertilizer on the profit of maize planting.
Gross income is an important economic index that determines the profit or benefit that a farmer can obtain. On the other hand, net return reflects the actual income of farmer. According to the average selling price of maize (1 yuan/kg) from 2018 to 2020, the net income of maize planting of different treatments was as follows: SFK45>SFK60>FGK60>FGK45>SFK30>FGK30>SFK0>FGK0>CK (Table 5). Compared to CK. the average net profit of maize planting in the three-year test increased by 421.26, 1049.07, 2014.82, 1980.44, 313.58, 1035.34, 1587.44, 1828.69 yuan/ha between the treatments of SFK0, SFK30, SFK45, SFK60, FGK0, FGK30, FGK45 and FGK60. Straw return and potassium supply increased the net profit of maize planting. The net profit of maize planting increased significantly after SF compared to FG under the same potassium supply. The treatment of SFK45 reached maximum profit of maize planting, which was 2014.82 yuan/ha.
Table 5. Effects of straw return methods and potassium fertilization levels on the profit of maize planting.
Treatment
|
Expenditure(yuan/ha)
|
Total expenditure(yuan/ha)
|
Yield
(kg/ha)
|
Gross income(yuan/ha)
|
Net profit(yuan/ha)
|
Straw returning
|
Potash fertilizer
|
Other
|
CK
|
0
|
0
|
1700
|
1700
|
11773.95
|
11773.95
|
10073.95
|
SFK0
|
750
|
0
|
1700
|
2450
|
12945.21
|
12945.21
|
10495.21
|
SFK30
|
750
|
120
|
1700
|
2570
|
13693.02
|
13693.02
|
11123.02
|
SFK45
|
750
|
180
|
1700
|
2630
|
14718.77
|
14718.77
|
12088.77
|
SFK60
|
750
|
240
|
1700
|
2690
|
14744.39
|
14744.39
|
12054.39
|
FGK0
|
450
|
0
|
1700
|
2150
|
12537.53
|
12537.53
|
10387.53
|
FGK30
|
450
|
120
|
1700
|
2270
|
13379.29
|
13379.29
|
11109.29
|
FGK45
|
450
|
180
|
1700
|
2330
|
13991.39
|
13991.39
|
11661.39
|
FGK60
|
450
|
240
|
1700
|
2390
|
14292.64
|
14292.64
|
11902.64
|