This article introduces the concept of a "nanoscale one-way valve" and its underlying principles, pointing out that permeation is a natural manifestation of the nanoscale one-way valve effect,the energy basis is the molecular thermal motion.Based on the principle and permeability mechanism of nanoscale one-way valve, the principle and method of zero-carbon emission reverse osmosis clean energy technology is proposed, the osmotic pressure difference is generated through the concentration regulation, and the spontaneous reverse osmosis is realized with the help of the spontaneous osmotic effect,intended to construct a one-way valve function,the disordered thermal motion of water molecules is converted into orderly motion, so that water can flow spontaneously water from low to high,to achieve the purpose of doing work directly taking energy from the thermal energy of water molecules without inputting energy,to achieve the goal of zero-carbon emission of clean energy. This paper introduces the experimental validation situation,The single valve can carry water about 2.8 meters high, which proves that the technical principle is feasible and the method is effective, which Capable of achieving water lifting without energy consumption. This research is a basic research, three basic breakthroughs were achieved:Reverses osmosis without energy consumption, let the water spontaneously flow from low to high, and using the heat of water for work without temperature difference. In the future, based on this principle and method,we will focus on this basis to improve work efficiency, which is expected to be practical quickly and achieve a new energy direction.There is no need for temperature difference, from the natural environment of water molecular heat energy can do work, inexhaustible, inexhaustible, do not consume energy resources, do not increase the temperature rise of the earth,zero-carbon emission , safe, clean and environmental protection, good economy.This study provides a new technical solution to effectively solve the focus problem of growing energy consumption and continuous global climate warming.