3.1.The result of the initial screening.
217 sequences were screened by virtual segmentation of SARS-CoV-2 genomes.Compared with human genome, 114 sequences were screened. The 114 sequences were compared with 26 primates' genomes to screen out 101 sequences. The 101 sequences were compared with 44707 SARS-CoV-2 genomes, they all met the experimental requirements.
3.2.Inhibitory effects of the screening
Compared with 44707 SARS-CoV-2 genomes, 18 sequences with over 95% matching rate and the number of bases is under 29 were screened out. 18 sequences and their highly similar sequences were matched with 44707 SARS-CoV-2 genomes.The combined matching rate was 100%, which was also the inhibition rate of the corresponding 18 main antisense oligonucleotides sequences.
3.3 Components of antisense oligonucleotides
5' UUAAUUGUGUACAAAAACUGCCAU 3'
5' UUACACGAUAACCAGUAAAGACAU 3'
5' CUAAUAAUGAGAUCCCAU 3'
5' CUAAGUCUGUGCCAGCAU 3'
5' UCAAUACUUGAGCACACUCAU 3'
5' CUAAAACAACACGAACGUCAU 3'
5' UUAACCUCUCUUCCGUGAAGUCAU 3'
5' UUAAAACACCCUCUUGAACAACAU 3'
5' UUACCUGUAAAACCCCAU 3'
5' UCAGUGAAUACUGUGAAAUUCCAU 3'
5' UUAAAUUCUUGGCAACCUCAU 3'
5' UCAGUACACCAACAAUACCAGCAU 3'
5' UUAGUAACAAAGGCUGUCCACCAU 3'
5' UUAACAAAAUCGCCCGUCUGCCAU 3'
5' CUACGUAUGCAAGCACCACAU 3'
5' UCAAUGGGCAAGCUUUGUCAU 3'
5' UUACAAACCAGUGUGUGCCAU 3'
5' CUAUAACGCAGCCUGUAAAAUCAU 3'