UTI is the most common disease caused by bacteria species in pregnant mothers which can lead to complicated disease and important complications in neonatal. Five bacterial species were found in pregnant women in Alsaudi and AMEC hospitals in Kassala. These were Escherichia coli, Streptococcus sp., Listeria sp., Neisseria sp. and Vibrio cholera. In Khartoum state hospitals, Ahmed et al. isolated E. coli, Klebsiella pneumoniae and Proteus mirabilis [5], Hamdan et al isolated K. pneumoni, P. aeruginosa, Escherichia. Coli, S. aureus and group B streptococcus isolated bacteria [7], Badri and Mohamed isolated E. coli, K. pneumonia, P. aeruginosa and E. feacalis [6] and Saeed et al. isolated Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus saprophyticus and Pseudomonas spp., as the urinary pathogens [12]. The overpopulation of this border city might cause the difference in bacteria isolated. This study revealed that the UTI in pregnant women in Kassala caused by bacteria is 70.9%. In Khartoum the prevalence of UTI was 14.0% [7] and 35% [12]. In Nigeria 61.5% and 56% recorded by [13, 9] respectively, while Assefa et al. [14] reported 11% in Ethiopia. The present study found that Listeria isolates were the most common (38.85%). In Khartoum E. coli was reported as the most common UTI causative agent [5, 7, 12].Also Escherichia coli is most common isolates in the other countries (9, 13, 15,16). The prevalence of Streptoococcus was also high (28.06%), rare cases causes by it [2]. Vibrio cholera (16.55%) and Neisseria sp (10.07%) were reported for the first time as UTI agent in Sudan, as the registered in Taiwan [17].less common isolates was Escherichia coli (6.47%).