Ubitidin-ribosomal protein plays an important regulatory role in protein synthesis, protein degradation, transcriptional processing, and apoptosis regulation, thus involved in plant growth and stress response. At present, no ubitidin-ribosomal gene has been reported in mango. In a previous study, a ubiquitin-ribosomal gene named MiUBRPS27a was obtained from cDNA-SCoT differential display analysis under different stress treatments of mango. In this study, the expression pattern and function of MiUBRPS27a were studied. The results showed that MiUBRPS27a was expressed in all tested tissues, among which the highest expression level was found in leaves. The expression of MiUBRPS27a gene was affected by low temperature, drought, and salt stress treatments. Overexpression of MiUBRPS27a significantly increased the root length in Arabidopsis under low temperature, drought and salt stress treatments. MiUBRPS27a transgenic plants showed decreased the content of malondialdehyde (MAD) and hydrogen peroxide (H2O2), increased the activity of superoxide dismutase (SOD) and proline content, thus increased the survival rate of transgenic Arabidopsis plants under drought stress. Our results suggest that MiUBRPS27a gene plays an important role in plant response to stress.