Effects of zone, Forages/forages and depth on Soil Organic Carbon and Soil Organic Nitrogen concentrations
The study revealed significant variations in soil organic carbon (SOC) and soil organic nitrogen (SON) stocks among different forages and crops, underscoring the influence of different forages on soil carbon and nitrogen dynamics. Panicum maximum (Panicum) exhibited the highest mean SOC stock at 20.50 Mg/ha, followed by Brachiaria spp at 18.28 Mg/ha, and maize (Zea mays) at 15.30 Mg/ha. This variation can be attributed to several factors related to plant characteristics and litter quality.
Research supports those perennial grasses like Panicum, with their extensive root systems and high biomass production, contribute significantly to SOC sequestration. These plants enhance organic matter input into the soil, promoting SOC accumulation through root turnover and litter decomposition processes (Zimmermann et al., 2012; Maass et al., 2015). In contrast, maize, being an annual crop with different growth patterns and litter quality, may contribute less to SOC formation due to lower quality residues and less extensive root systems compared to perennial grasses (Loss et al., 2013).
Although SOC varied significantly among the forages, SON stocks showed no significant differences. This suggests that while different plants may affect SOC accumulation differently, they may have similar impacts on SON stocks, possibly due to comparable nitrogen inputs from root exudates and decomposition residues across the forages (Yadav et al., 2019). The C ratio, which ranged from 3.3 to 5.5 across the different forages and crops, indicates variations in the decomposition rates of organic matter and nitrogen mineralization potential. Lower C ratios generally imply faster decomposition and potentially higher nitrogen mineralization rates, influencing soil fertility and nutrient availability (Sarkar et al., 2018; Wang et al., 2021).
Application of farmyard manure (FYM) was found to increase SOC content due to its carbon content and improved soil water holding capacity, essential for soil nutrient enhancement (Lehmann et al., 2020). FYM stimulates microbial populations and enhances soil conditions, accelerating organic matter decomposition and carbon release (Huang et al., 2019; Six et al., 2020). This enhancement in microbial activity contributes significantly to the buildup of soil organic carbon, crucial for soil fertility and ecosystem health. Farmyard manure (FYM) serves as a valuable source of soil nutrients and contributes to the improvement of soil physical structure (Lehmann et al., 2020). Application of farmyard manure (FYM) is a widely adopted method to enhance soil fertility by increasing soil available nutrients (Singh et al., 2020; Singh et al., 2021). Recent studies indicate that forage crops exhibit luxury consumption of nutrients when supplemented with inorganic fertilizers, depleting soil reserves due to the immediate availability of nutrients (Khan et al., 2019; Nigussie et al., 2020). This phenomenon underscores the importance of sustainable nutrient management practices in agriculture.
Forage crops like Panicum and Brachiaria exhibit substantial carbon sequestration potential, attributed to their robust root systems and efficient nutrient uptake (Smith et al., 2019; Wang et al., 2021). These findings underscore the importance of selecting appropriate forages in agricultural systems to enhance SOC levels and overall ecosystem resilience. Root-derived carbon inputs, rapidly absorbed and preserved within soil aggregates, further contribute to particulate organic matter and humus fractions, supporting long-term soil organic matter dynamics and carbon sequestration (Fornara and Tilman, 2008; Lehmann et al., 2020).
Effects of Zone, Forages/Maize (Zea mays) and Depth on Particulate organic carbon (POC) and Mineral Associated Organic Carbon (MAOC) content.
The above-ground dry matter contributes to increase in MAOC in the top layer (0–10 cm depth) of soil depth. The same was reported by Saraiva et al. (2014), that highest carbon stocks in the soil surface (0–10 cm depth) are due to the deposition of residues from the forage/crop in addition to root system concentrated into the top layers of soil.
The results obtained in this research indicate that Brachiaria spp and Panicum (Panicum maximum) showed an increase the POC content. This is attributable to high amount of residues above-ground and also direct influence of roots as the primary source of soil carbon and particularly to POC and MAOC as reflected in panicum and Brachiaria spp. plots. Thus, the greater below ground root biomass of the Brachiaria and panicum forages led to an increase in microbial activity and hence increased POC and MOAC as compared to Napier and Maize. This concurs with findings from Loss et al. (2013), indicating that the voluminous root systems of Panicum (Panicum maximum) and Brachiaria spp. contribute significantly to soil organic matter (SOM) accumulation. Recent studies continue to support this observation, underscoring the role of extensive root systems in enhancing SOM content and carbon sequestration (Kumar et al., 2020; Wang et al., 2021). Additionally, there is a higher canopy in the panicum and brachiaria resulting to higher soil moisture content, encouraged high litter turnover and thus increased SOC from the surface. Protective cover over the soil surface has been demonstrated to reduce the impacts of wind and water erosion on surface horizons (Devagiri et al., 2013). Higher levels of mineral-associated organic carbon (MAOC) are associated with the decomposition of plant residues and nutrient mineralization (Six et al., 2020; Lehmann et al., 2020). Previous studies have reported that Brachiaria spp. positively affects particulate organic carbon (POC) due to increased organic residue inputs into the soil (Cheruiyot et al., 2020). Moreover, Lopes et al. (2010) associated the higher MAOC content in Brachiaria spp. with greater shoot dry matter production and exudate release compared to other species.
From this research it is evident that POC was higher in the mid zone than other zones. This shows that forage/crop biomass changes with the zones and this highly impacts SOC input, hydrological processes and thus the effect on Particulate organic carbon and mineralized associated organic carbon. The mid zone, as characterized by Fernández-Romero et al. (2014), experiences distinct climate conditions that affect vegetation biomass and productivity, thereby influencing the quantity of organic carbon input into the soil. Recent studies continue to highlight the variability in climate conditions across different zones and its impact on soil organic carbon dynamics (Smith et al., 2020; Wang et al., 2021)
Research supports that different agro-ecological zones can significantly influence soil carbon fractions due to varying environmental conditions and management practices. For instance, studies by Zhao et al. (2018) and Smith et al. (2020) highlight how soil carbon levels can vary across different geographical zones, influenced by factors such as climate, soil type, and vegetation cover. The mid zone's higher SOC and POC levels align with findings by Peters et al. (2012), who emphasize the role of favourable environmental conditions and plant species in promoting carbon sequestration through increased root biomass and organic matter inputs.
Regarding MAOC, although no significant differences were observed among the three zones in this study, previous research by Wang et al. (2019) and Liu et al. (2021) underscores the stability of mineral-associated organic carbon across diverse environmental gradients. This stability suggests that while particulate organic carbon may vary with management practices and environmental conditions, mineral-associated organic carbon remains relatively consistent due to its bonding with soil minerals.
Regression analysis of MAOC with SOC, SON, POC, zones, forage, and depth
The correlations observed between agro-ecological zones and different forages in relation to soil particulate organic carbon (POC) provide insights into how environmental factors and plant species interact to influence carbon dynamics in agricultural soils. The inverse correlations between the mid zone and upper zone (r = -2.03E + 00 and r = -2.35E + 00; p < 0.001) with soil POC suggest contrasting impacts of these zones on carbon accumulation. This pattern aligns with studies that highlight varying soil carbon levels across different agroecological zones and management practices (Zhao et al., 2018; Smith et al., 2020).
Among the forages, maize and Napier grass showed inverse correlations with soil POC (r = -1.75E + 00 and r = -2.23E + 00; p < 0.05), indicating that these crops may have lower contributions to particulate organic carbon compared to other forages such as Panicum maximum and Brachiaria spp. This finding is consistent with research demonstrating the differential effects of plant species on soil carbon dynamics, influenced by factors such as root biomass, litter quality, and turnover rates (Loss et al., 2013; Kumar et al., 2020).
The positive correlations observed between the interaction of zones and forages (Mid Zone Maize r = 2.62E + 00, Mid Zone Nappier r = 3.53E + 00, Mid Zone Panicum r = 1.87E + 00, and Upper zone Panicum r = 3.16E + 00; p < 0.001) underscore how specific combinations of agro-ecological conditions and forage types can synergistically enhance soil POC levels. This synergy reflects the combined influence of favorable environmental factors and plant characteristics that promote carbon sequestration through increased root biomass and organic matter inputs (Peters et al., 2012; Kumar et al., 2020).
Furthermore, the positive correlation between Upper Zone Nappier and soil POC (p < 0.01) suggests that specific management practices or environmental conditions in the upper zone with Napier grass may also contribute positively to POC accumulation. This aligns with studies emphasizing the role of management strategies, such as organic residue management and soil conservation practices, in enhancing soil carbon storage (Devagiri et al., 2013; Lehmann et al., 2020).