Concerto in ASD: Classical Music on Increasing the Attention Span of Teenagers with Autism Spectrum Disorder

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Concerto in ASD: Classical Music on Increasing the Attention Span of Teenagers with Autism Spectrum Disorder

https://doi.org/10.21203/rs.3.rs-5174790/v1

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Autism Spectrum Disorder, also known as ASD, is a mental health disorder which affects one in thirty-six children in the United States. Characterized by the inability to focus, difficulties with social interactions, and short attention span, ASD thus has a negative impact on school performance for many teenagers diagnosed with this disorder. The purpose of this study was to see whether classical music, a form of stimulus which, according to prior research, was found to enhance the cognitive function of individuals, could potentially act as a catalyst for increased attention span in teenagers with ASD. This study hypothesized that classical music would increase the attention span and recurrence of on-task behavior of teenagers with ASD in the classroom setting. A mixed-method approach utilizing a questionnaire which employed quantitative four-point Likert scale data and qualitative observations by independent observers was used. This study was conducted on four teenagers diagnosed with ASD to see if classical music did increase their attention span, and both the qualitative and quantitative results seemed to indicate that classical music was indeed effective. Furthermore, the findings also hinted at long-term benefits of playing classical music in the background as teenagers with ASD learned in the classroom setting. Ultimately, the findings did confirm that classical music has a positive effect on increasing the attention span of teenagers with ASD, though it is recommended for more research to be conducted to confirm this positive correlation and see the effects of classical music on teenagers with ASD in the long-term.

ASD

attention span

classical music

on-task behavior

school performance

teenagers

According to the CDC, 1 out of 36 children are diagnosed with ASD, up from 1 in 44 in 2021 (Centers for Disease Control and Prevention, 2023). ASD, or Autism Spectrum Disorder, is a developmental disability and mental health condition characterized by repetitive behaviors, difficulties with social interactions, communication impairment, and short attention span (American Psychiatric Association, 2024; Chien et al., 2015). This last characteristic can be especially problematic for children, as they can have trouble receiving and retaining instructions and academic content in educational settings. Thus, it is not surprising that research has consistently shown that children who have ASD tend to do poorly in school which ends up impacting their future lives and career opportunities (Greenleaf, 2018).

This can be especially concerning for teenagers with ASD, as puberty also brings many changes to not just their physical bodies but also to their cognitive function. However, due to the unique challenges ASD poses, teenagers with this mental health condition can have very different and even adverse developments in their brains during this time, which is why it is of the utmost importance to prevent this concerning trend and find methods to foster and encourage on-task behavior (Goddings, 2019). Still, one form of stimuli may be the potential solution to this issue: classical music.

Classical music has long been shown to enhance the cognitive function of listeners, leading to the general public and media erroneously associating it to increased IQ points (Mammarella et al., 2007). Still, research has suggested that classical music has the added benefit of increasing the attention span of listeners and aiding their ability to concentrate and focus, and it is entirely possible its benefits can be extended to teenagers with ASD.

This paper aims to see if classical music is able to increase the attention span of teenagers with ASD, and will discuss the results, as well as the limitations and implications, of the conducted research.

Classical music refers to the music written in the European tradition between the period 1600 to 1900, including works from various European composers such as Bach, Mozart, and Tchaikovsky (Bell et al., 2016). Over the past few years, there has been an emphasis on how classical music can have a significant impact on one's mental function and can be used to reap therapeutic benefits.

Classical Music on Cognitive Function

The existing body of literature shows that classical music — specifically, music composed by the composer Wolfgang Amadeus Mozart and Antonio Vivaldi — has a positive impact on one’s cognitive function (Rauscher et al., 1993; Mammarella et al., 2007). For instance, Rauscher and colleagues’ research found that the spatial reasoning of college students after listening to a sample of Mozart’s Sonata for Two Pianos showed a temporary increase, as indicated by enhanced performance on the spatial attention section of the Stanford-Binet IQ test (Rauscher et al., 1993). However, this effect, dubbed the “Mozart Effect,” was shown to be temporary, with no effects after the fifteen-minute mark (Rauscher et al., 1993). Similarly, Mammarella and colleagues explored the “Vivaldi Effect,” in which a cohort of senior citizens completed the Digit Span Test and the Word Fluency Test — two working memory tasks — with one of three conditions: listening to white noise, listening to an excerpt of Vivaldi’s Four Seasons Concertos, or listening to no music (Mammarella et al., 2007). The researchers discovered that there was a “a greater level of attention [span]” and a significant increase working memory performance in participants who were listening to Vivaldi’s Four Seasons than both listening to white noise and listening to no music as shown through higher scores in the two tasks, demonstrating classical music’s ability to enhance the attention span and working memory of listeners (Mammarella et al., 2007).

However, though these two studies looked at college students and older adults respectively, their findings could potentially be extended to benefit a different demographic — specifically, children with Autism Spectrum Disorder.

Increasing Attention Span of Children with ASD

Research has been done to find ways to increase the attention span of children with ASD and mitigate the negative effects of ASD’s symptoms in the classroom setting. For example, functional play intervention (FPI), the action of a child exploring and directly interacting with objects as they are intended to be used, coupled with specific verbal probes from the classroom teacher was implemented in a specialized classroom for children with ASD (Basso et al., 2020). Basso and colleagues found that this implementation of FPI and verbal probes enhanced the joint attention of the students with ASD and helped the students focus on the tasks at-hand (Basso et al., 2020). Furthermore, another study found that the integration of physical activity into teaching sessions with students diagnosed with ASD was shown to improve their attention span significantly, indicated by higher test scores and qualitative observations made by the teacher (Tan, 2014). Similarly, Holifield and colleagues found that by implementing a unique self-monitoring procedure where visual cues and verbal aid were combined, children with ASD were able to increase their abilities in better attending to classroom tasks, correlating to an increase in their attention span and classroom performance (Holifield et al., 2010). They also discovered that continued implementation of this intervention method resulted in long-term benefits of increased attention span (Holifield et al., 2010). Yet another notable study, this time specifically regarding classical music implementation in the classroom, experimented with playing classical music in the background while three autistic children received lessons from a teacher (Greenleaf, 2018). This study by Greenleaf, which is also my seminal source for this paper, found that classical music effectively increased on-task behavior in children with ASD (Greenleaf, 2018). Thus, it was shown that music is able to induce positive psychological benefits such as on-task behavior and attention span on children with ASD.

Taken as a whole, this varying literature not only confirms the possibility of boosting the attention span of children with ASD but also explains how different practices achieving this purpose can be implemented in the classroom setting.

Teenagers with ASD

Still, although research has been done on the effects of classical music on children with ASD, there still remains a gap in the literature in regards to focusing on a different demographic — specifically, teenagers with ASD. Since teenagers — humans between the ages 13 to 17 — are individuals undergoing puberty, they experience both physical and mental changes, one of which being the further development of their neural networks and attention span (Konrad et al., 2013; Blakemore et al., 2010). Brain maturation occurs during puberty due to a surge in the synthesis of hormones such as testosterone, and a significant implication is that the attention span of a teen continues to evolve and becomes more like that of an adult’s (Arain et al., 2013). In general, teenagers also become better at retaining information and learning in the classroom setting; however, due to the unique challenges ASD poses, teenagers with this mental health condition can have very different and even adverse developments in their brains during this time, potentially further hindering their ability to pay attention in the classroom (Goddings 2019).

Still, as it has been previously observed by Greenleaf that classical music can improve the attention span of children with ASD, there is thus value in seeing whether classical music’s benefits can also aid teenagers with this mental health condition. This exact discrepancy between children and teenagers with ASD — as the latter demographic experience unique cognitive changes during puberty — begs the question, “through a mixed-method approach utilizing both qualitative observations and quantitative measures of attention levels, does classical music help teenagers with ASD lengthen their attention span?”

This study used a qualitative approach to measure on-task behavior in teenagers with ASD by utilizing four-point Likert scale surveys, and is adapted from the methodology used by Greenleaf (Greenleaf, 2018). The study consisted of a control and intervention stage. The former was a means of gauging students’ initial behavior in the classroom with no music playing, while the latter was when classical music played in the background and the Likert scale survey was conducted.

The Playlist

The curated classical music playlist included various different pieces from the three eras, including the works of Tchaikovsky, Mozart, Chopin, Debussy, Bach, and more. The pieces which were used in the playlist were the following:

P. I. Tchaikovsky, Serenade for Strings in C major, mvt. 2, “Valse”
F. Chopin, Nocturne No. 2 in E♭ major
L. V. Beethoven, Symphony No. 5, mvt. 2
C. Debussy, Suite Bergamasque, mvt. 3, “Clair de Lune”
A. Vivaldi, Concerto for 2 Violins in A minor, mvt. 1
W. A. Mozart, Serenade No. 13 in G major, mvt. 1, “Eine Kleine Nachtmusik”
A. Vivaldi, Concerto No. 1 in E major, mvt. 1, "Spring"
J. S. Bach, Brandenburg Concerto No. 3 in G major, mvt. 3
W. A. Mozart, Piano Sonata No. 16 in C major

The playlist represents a wide range of classical music, with three pieces from the Baroque Era, three from the Classical Era, and three from the Romantic Era. It is also a mix of both solo and orchestral/chamber string pieces, with five being solo pieces and the rest being orchestral and chamber strings. The purpose of this curated playlist is to give students a diverse sample of classical music, especially considering the fact that “classical music” is an umbrella term encompassing Western European music from the Baroque, Classical, and Romantic Era. These works were chosen as each offers a different aspect of classical music. For example, Tchaikovsky’s selection is in the style of a waltz, characterized by ¾ time, while Vivaldi’s Concerto No. 1 is a chamber string solo piece and Beethoven’s Symphony No. 5 is an orchestral work, containing parts for strings, brass, woodwinds, and percussion. In total, the playlist’s duration is around 49 minutes and 51 seconds, which was roughly the length of a lesson. Every day during the intervention stage, the playlist was played in the same order to emphasize consistency. However, as it is difficult to “standardize” classical music and no studies have been done on the specific aspects of classical music which increase attention span, the playlist may potentially be a limitation of the methodology.

Sample Size

This study was performed in collaboration with an autism center located in Singapore, which offered to help provide a class where this study could be conducted. The observer group consisted of three faculty members from the centers who were individuals trained in data collection and working with teenagers with ASD (Greenleaf, 2018). Their task was to see whether the classical music intervention was effective in increasing on-task behavior in teenagers with ASD through the use of the Likert scale survey. Meanwhile, the participant group was comprised of four thirteen-year-old students who were confirmed that they had ASD based on official medical diagnoses. Out of the four students, three were male and one was female. They were all capable of verbal communication, though some chose to employ hand gestures instead. Additionally, as per privacy guidelines, this research paper will employ pseudonyms when referring to specific individuals to protect their identity. Both observers and participants had all signed an Informed Consent Form and had agreed to participate in this study (see Appendix B).

Control Stage

During the control stage, the observers watched and took notes on the students’ behaviors. They were told to do this for all of the participants, as this was to gauge students’ behavioral characteristics during a typical class (Greenleaf, 2018). During the intervention stage, the curated playlist mentioned above was played while the students were participating in a variety of activities, such as reading, drawing pictures, and listening to the teacher. The same playlist as mentioned above was used during each session in the same order for approximately one hour, which was the duration of the session. The music was playing from a laptop at the back of the classroom with the music playing at a volume loud enough to hear, but quiet enough that students could hear the teacher speaking (Greenleaf, 2018). Additionally, since ASD patients may be sensitive to sound, students were given the opportunity to use noise-canceling headphones.

Intervention Stage

After each day during the intervention stage, the observers filled out a four-point Likert scale survey adapted from Berger and colleagues’ research, which explored the psychometric properties of the Scale of Treatment Perceptions — a measure of the efficacy of treatments targeting skill-building interventions for students with ASD and is the same survey used by Greenleaf in her research (Berger et al., 2016; Greenleaf, 2018). The observers would rate on a scale of 1 to 4 (with 1 being “strongly disagree” and 4 being “strongly agree”) each statement based on their observations in the classroom (see Appendix A). For the purpose of this study, attention span is measured using three characteristics: eye contact, listening skills (i.e. responding when prompted by the teacher), and reduction of distracting noises, as these are the main characteristics of proper attention span (Simon et al., 2023). Thus, the prompts in the survey were specifically made to measure these characteristics (Berger et al., 2016). Additionally, the observers were provided with space to note down qualitative observations, hence the mixed method approach.

Statistical Analysis

Once the Likert scale data is collected, it is statistically analyzed using the Wilcoxon signed-rank test (Berger et al., 2016; Greenleaf, 2018). Given the ordinal nature of Likert-scale data and the relatively small sample size (n = 9), the Wilcoxon signed-rank test was deemed more suitable over the paired t-test used by Berger and colleagues’ study. This is due to the non-normal distribution of the data (i.e. normality could not be assumed) and the robustness of the Wilcoxon signed-rank test, ensuring the integrity of the statistical analysis and the reliability of the obtained results. Thus, this statistical test determines the statistical significance of the data and shows whether the classical music intervention was effective or not.

To perform the Wilcoxon signed-rank test, the mean values of the responses for Day 3 and Day 1 must be found, then subtracted from one another. The reason why Day 2 was excluded was because the most significant changes occurred between Day 1 and Day 3, representing the first and last day of the study, respectively. Then, the sum of the positive ranks (W⁺) and negative ranks (W^–) must be determined by adding the positive mean values for W⁺ and the absolute value of the negative mean values for W^– (Berger et al., 2016; Greenleaf, 2018). Of the two sums of the ranks, the smaller value is taken to be the test statistic t_statistic, which is compared to the critical value for a one-tailed test with n = 9 and 𝛼 = .05, which is p = 8. If the test statistic is greater than the critical value, I fail to reject the null hypothesis H₀, which is that there is no statistical evidence which indicates that classical music increased the attention span of the teenagers. Alternatively, if the test statistic is smaller than the critical value, I succeed in rejecting the null hypothesis, and an alternative hypothesis H_a is adopted, that being that classical music did indeed increase the attention span of the teenagers.

Quantitative Results

After the duration of the study, all of the survey responses on all three days were collected, and can be summarized in the tables shown in Appendix C (see Appendix C). The first portion of the results consisted of the Likert scale data, with the Wilcoxon signed-rank test being performed. Firstly, the mean values of the responses for Day 3 and Day 1 were found for each question, as shown in Table 1.

Table 1

*Mean values of Day 3, Day 1, and their arithmetic differences.*
	$\:{\underset{\_}{x}}_{Day\:3}\:$	$\:{\underset{\_}{x}}_{Day\:1}\:$	$\:{\underset{\_}{x}}_{Day\:3}\:{-\underset{\_}{x}}_{Day1}\:$
Question 1	2	2.333	− .333
Question 2	2.667	2.667	0
Question 3	3	2.333	.667
Question 4	3.333	2.333	1
Question 5	3	2.67	.333
Question 6	3.667	3	.667
Question 7	2.667	2.333	.333
Question 8	3	3	0

Then, the sum of the positive ranks (W⁺) and negative ranks (W^–) were found, respectively.

$$\:{W}^{+}=\varSigma\:\left|{\underset{\_}{(x}}_{Day\:3}\:{-\underset{\_}{x}}_{Day1}{)}_{positive}\right|=.667+1+.333+.667+.333=3$$

$$\:{W}^{+}=\varSigma\:\left|{x}_{negative}\right|=.333$$

As $\:{W}^{-}$ is smaller than $\:{W}^{+}$, it is the value of the test statistic, with t_statistic = .333. Now, to determine the critical value for this test, I used a table of critical values for the Wilcoxon signed-rank test. For a one-tailed test with n = 9 and 𝛼 = .05, the critical value is p = 8. As t_statistic is smaller than p (.333 < 8), the null hypothesis H₀ was successfully rejected. In other words, statistically speaking, the classical music intervention seemed to improve the attention span of the children.

Qualitative Results

The second half of the data consisted of the comments and observations made by the observers and can be summarized in the table in Appendix D (see Appendix D). Overall, the general consensus was that classical music was effective in increasing the attention span of teenagers with ASD. As mentioned before, during the control stage, observers took notes on all four students. Firstly, for Alex, a thirteen-year-old Indian male diagnosed with ASD, the observers noted down that he was very vocal, loses eye contact frequently, and fidgets with his chair. They also found that he participates often in class, asking many questions and responding to prompts made by the teacher.

Secondly, David, a thirteen-year-old Chinese-Singaporean male diagnosed with ASD, was observed to be distracted at times and likes to put his head down on the desk. The observers also noted that he enjoys doodling and likes to draw company logos on paper.

Our third participant is Hector, another thirteen-year-old Chinese-Singaporean male diagnosed with ASD. Though he had the potential to speak words, Hector was mostly non-verbal, using an app on his iPad to communicate. The observers described him as being attentive but “slow,” not quickly responding to stimuli from the teacher by not asking questions, providing answers, or engaging in activities.

Finally, the fourth participant is Carolyn, a thirteen-year-old Chinese-Singaporean female diagnosed with ASD. Observers noted that she was very “tech-savvy” and can “catch on to things fast,” often helping her teacher with tech-related issues such as when the projector wasn’t working. Still, the observers also reported that she spent a lot of time on her phone, watching videos rather than focusing on the lesson. She also didn’t speak much, often using hand gestures instead of words to communicate, though she did make “angry sounds” when her phone was confiscated.

During all three days of the intervention stage, the observers also continued to make qualitative observations on the students. On the first day, one of the observers pointed out that Alex “seemed slightly distracted by the music” and that he “touched the computer for a bit [and started] walking around.” For David, “not much change” was observed as he continued to doodle during class. Hector was also “unresponsive to [the] music” on the first day, with one observer describing him as being “zoned out most of the time.” For Carolyn, one observer noted that she “seemed quite distracted by the music… and was more hyperactive than before.”

On the second day, there were some observable changes in the teenagers. One observer noted that Alex “made less humming sounds,” signaling a potential increase in his attention span. Similarly, David was reported as seeming “to be a bit quieter,” though one observer mentioned that a “conclusion cannot be drawn at this point.” By contrast, Hector still “continued to be quiet” and there was “not much change from [the] control stage.” Carolyn “still used [her] phone,” but apparently “seemed calmer and seemed to listen more attentively.”

Finally, on the third day, one observer mentioned that Alex, one of the participants, “hummed less [and was] more focused during [the] session.” Similarly, David was reported to be “relatively focused” on the third day and made “more eye contact.” On the other hand, another observer mentioned that Hector, a “very quiet” student, didn’t change much after the three days even with the music, saying that he “continued to be quiet; [there was] not much change from [the] control stage.”

Teenagers with ASD struggle with maintaining their attention spans in the classroom setting, especially with the onset of puberty and radical cognitive developments. Thus, this study was conducted to determine whether classical music could act as a potential intervention method and increase the attention span of students of this demographic. Based on the current body of literature, it was theorized that classical music would be an effective means of aiding teenagers with ASD in paying attention in the classroom setting.

The results of this study shed light on some of the potential ways classical music’s therapeutic benefits can be harnessed in the classroom. As a whole, the students’ attention span was significantly improved by the end of the intervention stage. By the third day, the observers reported an overall increase in both participation and eye contact with the teacher, as well as a decrease in the production of distracting noises, thus proving that classical music was indeed effective. These qualitative observations also correlate to the Likert scale responses, which quantified the positive effect of classical music. The alternative hypothesis H_a, which stated that there was a positive correlation between classical music intervention and increased attention span, was adopted after it was found that the test statistic was smaller than the critical value. The results seemed to confirm my initial hypothesis that classical music will indeed increase the attention span of teenagers with ASD.

Furthermore, the results fill in the void present in the literature and also drives the research forward. For instance, the results are similar to Greenleaf’s findings, which suggested that classical music was able to increase the on-task behavior of children with ASD (Greenleaf, 2018). Now, with my results, the relationship between classical music’s benefits and the attention span of children with ASD can be further extended to their older, more complex counterparts: the attention span of teenagers with ASD. Additionally, the results are similar to some of the other methods used by researchers to enhance the attention span of children with ASD, such as Basso and colleagues’ implementation of functional play intervention with verbal probes and Tan’s integration of physical activities in the classroom setting (Basso et al., 2020; Tan, 2014).

Yet, it is important to note the nuances in the results. As noticed by the observers, the classical music stimulus was not effective for all the participants. For example, Hector wasn't very verbal to begin with, and music didn't seem to "have a positive or negative effect on him.” However, for Alex, Carolyn, and David, while they were a bit distracted on the first day of the session, they eventually showed positive reactions to the classical music stimulus with a reported increase in their attention span. This can potentially be attributed to the inherent nature of autism. As the disorder itself lies on a spectrum, the effects it has can vary widely for different individuals, often resulting in slight variations and fluctuations in regards to its symptoms and the extent to which it impacts one’s attention span (Greenleaf, 2018).

Another notable finding is the fleeting nature of classical music’s effects. According to the responses on the Likert scale, two of the observers had selected “disagree” to the statement that “the student's attention span remained at an improved level even after the treatment ended.” This notion that classical music’s effects may persist after the intervention ends contradicts the finding in Rauscher and colleagues’ study, which showed that the enhanced spatial attention of the participants did not last longer than fifteen minutes after the music was turned off (Rauscher et al., 1993). However, this may potentially be attributed to the observers’ perceptions of what is considered as “improved levels” and how the length of maintained attention span after the intervention wasn’t timed, unlike the case with Rauscher and colleagues’ study. Thus, more research must be performed to address this slight discrepancy between these results. Still, the results may also hint at the potential for long-term benefits of consistently listening to classical music, especially considering that the observers reported that increased attention span was maintained even after the intervention ended. Additionally, the benefits of classical music on attention span seemed to increase over time according to both the Likert scale data and the qualitative observations made by the observers. Interestingly, this correlates to the discoveries made by Holifield and colleagues’ research, where they found that their unique self-monitoring procedure resulted in long-term benefits for the attention span of children with ASD (Holifield et al., 2010).

It can thus be concluded that classical music does indeed boost the attention span of teenagers with ASD and is an effective means of doing so.

Limitations

However, there are some limitations to the conclusion. Firstly, due to the inherent nature of ASD as a disorder on a spectrum, the severity and symptoms of the disorder vary for many individuals — such as Hector and his unresponsiveness to the music — meaning that the extent and efficacy of classical music stimuli also varies and should be dealt with on a case-by-case basis, such as the use of a different playlist or longer duration of the intervention. Furthermore, as this research looked only at one classroom in one autism center, the results and findings are only applicable to this one classroom and may not necessarily be extrapolated for other autism centers which have significant differences such as a different teaching curriculum, different class sizes, and varying degrees of ASD severity of students.

Implications

Still, despite some of these potential limitations, the results of this study have some significant implications. As the implementation of the classical music playlist is quite easy and only requires a device and a working Wi-Fi connection in the classroom, it proves to be an effective and cost-efficient method for teachers in the autism center I worked with to improve the attention span of teenagers with ASD. Thus, it becomes a way to enhance concentration and attentiveness.

Future Research

In regards to future research, studies need to be conducted to see whether these findings and results hold true for other autism-centered classrooms. Additionally, as mentioned above, the results seem to hint at potential long-term gains which may appear from the teenagers consistently listening to classical music in the classroom setting over a longer period of time. This can further be explored if a study with a longer intervention duration was conducted. Thus, this calls for future research to be done to see if the results are consistent for other autism-centered classrooms, as well as whether the findings of this paper hold true in the long-term.

Overall, the results of this study point to the conclusion that classical music is indeed effective in increasing the attention spans of teenagers with ASD and has both limitations and significant implications, though more research needs to be done to confirm or deny these findings.

Data Availability Statement

The data used in this research paper can be found and summarized in Appendix C and D after the References section. Please refer there for comprehensive information regarding data collected throughout the duration of the study. Names have been changed to ensure participant anonymity.

Compliance with Ethical Statement

Prior to the research the author has submitted a form to the Institutional Review Board (IRB) of Singapore American School. The research has been approved by the appropriate authorities and deemed to be in compliance with legal and ethical regulations.

Conflict of Interest Statement

The author conducted the study in partnership with St. Andrew’s Autism Centre located in Singapore. He received no financial compensation from the partnership, and thus has no conflicts of interest to declare.

Informed Consent Statement

The author had taken steps to ensure that participation in the study was voluntary with the requirement of an Informed Consent Form (ICF). ICFs had been collected from all participants prior to the study, and can be found under Appendix B.

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No competing interests reported.

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Concerto in ASD: Classical Music on Increasing the Attention Span of Teenagers with Autism Spectrum Disorder

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Abstract

Introduction

Literature Review

Classical Music on Cognitive Function

Increasing Attention Span of Children with ASD

Teenagers with ASD

Methodology

The Playlist

Sample Size

Control Stage

Intervention Stage

Statistical Analysis

Results

Quantitative Results

Qualitative Results

Discussion

Conclusion

Limitations

Implications

Future Research

Declarations

References

Additional Declarations

Supplementary Files

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