With the introduction of the phenotype of ‘Metabolic syndrome-associated osteoarthritis’ (MetS-OA) and ‘diabetes-induced-osteoarthritis’(DM-OA)[18–19], researchers have increasingly focused on the link between OA and metabolism in recent years. However, the effect of insulin resistance on the progression or mortality of OA remains controversial [20–21]. This study explores the relationship between different IR surrogates and all-cause mortality in a community-based population with OA in the United States. Using multivariate Cox regression and RCS analysis, we determined that HOMA-IR is a reliable predictor of all-cause mortality risk in patients with OA, displaying a U-shaped association with mortality risk; both high and low levels of HOMA-IR increased the risk.
The baseline characteristics of the study population indicated that participants who died during follow-up were more likely to be older, have a lower education level and income, be more smokers, diabetes, heart failure, stroke, and cancer patients. In segmented multivariate Cox regression analysis, when HOMA-IR was modeled as a continuous variable, the effect values were statistically significant. However, when HOMA-IR was modeled as categorical variables, the effect values were not statistically significant, possibly due to decrease testing efficiency resulting from insufficient sample size for each group.
IR is an important feature of the MetS, and alongside diabetes, obesity, and dyslipidemia, it is also a risk factor for the development of OA and may be associated with adverse outcomes in patients with OA [22]. Aging and obesity have been long established as predominant and possibly preventable risk factors for OA. They not only enhances the load on the weight-bearing joints [23] but also causes misalignment and unfavorable joint mechanics, particularly in the knees, thereby increasing mechanical stress and cartilage degradation leading to OA [24]. However, altered biomechanics do not fully justify the increased risk for OA in non-weight-bearing joints such as the hands and the wrists in obese subjects, pointing to a systemic, non-mechanical influence on the risk for OA [25]. Our study also indicated that OA participants who died did not exhibit higher BMI. Recent studies have drawn a link between OA and MetS, which is characterized by IR. Some studies have found that the presence of IR and OA interact with each other, further complicating the management of OA. A UK Biobank prospective cohort study found that MetS and its components, which include hyperglycemia and dyslipidemia, were associated with an increased risk of OA, particularly in individuals with elevated levels of CRP [26]. The hyperinsulinemic-normal glucose clamp technique is the gold standard for the diagnosis of IR, but is difficult to use in large-scale clinical studies due to its imitations. Therefore, various different IR surrogates, including HOMA-IR, TyG index and TyG-BMI index, are widely used in clinical research.
Previous studies have extensively examined the relationship between IR and OA morbidity, but research on IR and OA outcomes remains limited. For instance, a cross-sectional study involving 3921 patients demonstrated that an increased risk of OA was associated with a higher TyG index, with each incremental unit increase in the TyG index correlating with a 634% higher risk of OA [OR = 7.34; 95% CI: 2.25, 23.93; p = 0.0010] [13]. Additionally, a study from Germany indicated that musculoskeletal impairment and osteoarthritis-related symptoms are linked with insulin resistance [27]. However, our study did not find a significant association between the TyG index and all-cause mortality, possibly due to variations in the follow-up duration. No previous research has explored the correlation between the TyG-BMI index and prognosis in patients with OA, and our findings did not reveal a significant correlation. As for HOMA-IR, our analysis identified it as a robust predictor of all-cause mortality in patients with OA. Specifically, when HOMA-IR was less than 3.72, it was inversely associated with mortality risk; conversely, when HOMA-IR exceeded 3.72, it was positively associated with all-cause mortality, indicating a U-shaped relationship between HOMA-IR levels and mortality in patients with OA. The model evaluation results further supported that HOMA-IR’s strong predictive ability for all-cause mortality. While prior evidence suggests a possible link between OA and diabetes, reinforcing the notion that diabetes might be an important independent risk factor for OA[28], the relationship between IR and OA has not been quantitatively demonstrated through clinical data, nor has the nonlinear relationship been assessed. Our study addresses these gaps by providing new insights into the complex interplay between IR and OA outcomes.
It is important to highlight that immune factors may play a concealed role behind IR substitutes. The metabolic reprogramming of fibroblast-like synoviocytes (FLS) during OA progression is an emerging area of research. Before pathological damage to the articular cartilage is evident, FLS may undergo glucose metabolism reprogramming under the internal environmental disturbances, which is characterized by a shift to inefficient glycolysis despite the presence of sufficient oxygen, a phenomenon also known as Warburg Effect originally identified in tumor cells [29]. This increase glycolysis in FLS leads to the infiltration and polarization of synovial macrophage, significantly elevating the expression of pro-inflammatory mediators such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α, cyclooxygenase (COX)-2, and matrix metalloproteinases (MMP)-3, -13[30]. These pro-inflammatory cytokines not only expand the scope and scale of inflammation, but also synergize to exacerbate the adverse effects in OA including upregulating the expression of proteases, aiding in the degradation of cartilage extracellular matrix (ECM), and ultimately leading to total joint failure [31]. Normally, Insulin could suppress this inflammatory process[32]. However, the abnormal glucose metabolism in FLS and chondrocytes leads to impaired regulation of glucose transporters (GLUT) and a diminished ability to respond to extracellular glucose levels [28,32–34]. Furthermore, adipose tissue (AT) also acts as a significant source of pro-inflammatory cytokines, contributing to low-grade chronic metabolic inflammation [35]. This type of inflammation can exacerbate joint structural damage and is frequently associated with IR and T2DM [36,37].
It is essential to explore the relationship between IR and the mortality of OA. IR contributes to several pathological conditions including endothelial dysfunction, abnormal lipid metabolism, hypertension, and systemic inflammatory, all of which are closely related to the development and adverse outcomes of OA [38]. MMPs can be synthesized and released by the promotion of IR through various pathways [8], which are crucial in the osteogenic transformation and medial artery calcification in vascular smooth muscle cell(VSMC). This process potentially leads to significant cardiovascular events[39,40]. Our study observed that participants who succumbed during follow-up exhibited higher incidences of heart failure and stroke. Furthermore, IR facilitates the production of advanced glycation end products(AGEs), which are significantly involved in the progression of OA and the occurrence of CVD. Accumulation of AGEs leads to increased brittleness of the cartilage, promote matrix stiffness and make the cartilage more sensitive to mechanical stress which result in degradation[41]. AGEs also impair the AMPKα/SIRT1/PGC-1α signaling pathway in chondrocytes, resulting in mitochondrial dysfunction as a result of increased oxidative stress, inflammation, and apoptosis[42]. Additionally, studies indicate that AGEs could upregulate the expression of MMPs in fibroblasts, contributing to myocardial fibrosis[43]. Our study found that the HOMA-IR can serve as an effective predictor of all-cause mortality risk in patients with OA. Both high and low HOMA-IR levels were associated with an increased risk of all-cause mortality, indicating a potential tool for clinicians to assess and manage mortality risk in OA patients proactively.
It is worth noting that HOMA-IR, as one of the IR surrogates, has demonstrated robust predictive capabilities for mortality in diseases related to MetS, including coronary heart disease[44], heart failure[45] and nonalcoholic fatty liver disease [46]. It’s necessary to investigate whether HOMA-IR’s association with all-cause mortality remains consistent across different diseases to broaden its clinical applicability.
The strengths of our study include the out study benefits from a nationally representative sample of the U.S. multiethnic population. Besides, the appropriate NHANES sample weights and covariates were considered while performing the analyses to minimize the impact of confounding variables and improve the generalizability of our findings to the general population.
However, several limitations must be acknowledged. Firstly, the diagnosis of OA in this study was obtained through self-reporting, lacking critical laboratory tests like anti-Cyclic Citrullinated Peptide antibodies and Rheumatoid Factor due to NHANES database restrictions, potentially introducing bias in OA diagnosis and differential diagnosis. Furthermore, NHANES does not collect longitudinal data on key factors such as changes in the metabolic abnormality, which may affect long term outcome, including mortality. Finally, converting HOMA-IR into a categorical variable for analysis and performing segmented stratified analysis may reduce the sample size in each group, thereby decreasing the efficiency of tests.