Ethics approval and consent to participate
The Ethics Committee of the Reina Sofia University Hospital (Córdoba, Spain) approved the study, which was conducted in accordance with the Declaration of Helsinki. Written informed consent was signed by every patient. FFPE samples were obtained from the Andalusian Biobank. Patient samples and data used in RNA-seq were collected from the ARC‐Net Research Centre, University of Verona, Italy, under approval number CE2172 (Prot 26773) from the Integrated University Hospital Trust Ethics Committee. All procedures used in the zebrafish experiments, fish care and treatment were performed in agreement with the Animal Care and Use Committee of the University of Santiago de Compostela and the standard protocols of Spain (Directive 2012-63-DaUE). Mice xenograft experiments were performed according to the European-Regulations for Animal-Care under the approval of the University of Cordoba research ethics committees.
Consent for publication
Not applicable.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Competing interests
The authors declare no competing interests regarding this study
Funding
This work has been supported by Spanish Ministry of Economy [MINECO; BFU2016-80360-R (to JPC)] and Ministry of Science and Innovation [MICINN; PID2019-105201RB-I00 (to JPC), PID2019-105564RB-I00 (to RML)]. Instituto de Salud Carlos III, co-funded by European Union (ERDF/ESF, “Investing in your future”) [FIS Grants PI17/02287 and PI20/01301 (to MDG), PI18/00757 (to BS,Jr);DTS Grant DTS20/00050 (to RML)); Postdoctoral Grant Sara Borrell CD19/00255 (to AIC); Predoctoral contract FI17/00282 (to EAP)]. Coordinated grant (GC16173694BARB) from the Fundación Asociación Española Contra el Cáncer (AECC) (to BS,Jr). Spanish Ministry of Universities [Predoctoral contracts FPU14/04290 (to SPA); FPU16/06190 (to VHA); FPU18/02275 (to RBE)]. Boehringer Ingelheim Fonds travel grant (EAP). Junta de Andalucía (BIO-0139). GETNE2016 and GETNE2019 Research grants (to JPC); and CIBERobn. Associazione Italiana Ricerca Cancro (AIRC 5x1000 n. 12182); Fondazione Italiana Malattie Pancreas – Ministero Salute [FIMPCUP_J38D19000690001]; Fondazione Cariverona: Oncology Biobank Project “Antonio Schiavi” (prot. 203885/2017).
Authors’ contributions
EAP: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing - original draft, Writing - review & editing.
RBE: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing -review & editing.
SA: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing -review & editing.
CVG: Methodology, Validation, Resources, Writing - review & editing.
SPA: Data curation, Formal analysis, Investigation, Writing - review and editing.
VHA: Data curation, Formal analysis, Investigation, Writing - review and editing.
JMJV: Data curation, Formal analysis, Investigation, Writing - review and editing.
AM: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
MESF: Methodology, Formal analysis, Validation, Resources, Writing - review & editing.
MTC: Methodology, Validation, Resources, Writing - review & editing.
FAJ: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
JAMS: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
PCS: Investigation, Methodology, Data curation, Formal analysis, Investigation, Writing - review and editing.
RT: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
LS: Investigation, Methodology, Data curation, Formal analysis, Investigation, Writing - review and editing.
JMJH: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Resources, Writing - review & editing.
SV: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
LMH: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing -review & editing.
MDG: Formal analysis, Methodology, Resources, Writing – original draft, Writing - original draft, Writing - review & editing.
AS: Data curation, Formal analysis, Investigation, Software, Writing – review and editing.
AAS: Conception, Data curation, Formal analysis, Investigation, Methodology, Validation, Resources, Writing - review & editing.
AIC: Formal analysis, Methodology, Resources, Writing – original draft, Writing - original draft, Writing - review & editing.
BSJr: Conception, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
RML: Conception, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
JPC: Conception, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
Acknowledgements
Authors gratefully acknowledge Dr. FX Real who generously provided the HPDE E6E7 cell line, and all patients for generously donating the samples studies in this work.