4.1 Physical Properties of selected chickpea cultivars
The physical properties of different chickpea cultivars are shown in Figure 1. Significant difference was seen in the 100-grain weight of desi and kabuli varieties of chickpea. Desi variety shows the weight range 18.19±0.42 to 27.08±1.03g while kabuli variety shows higher value (26.71±0.58 to 33.98±0.50 g) per 100-grain weight. The diameter of chickpea seeds ranged from 4±1 to 7.5±0.5mm. K-850 shows the higher seed diameter (7.5±0.5mm) while BG-3062 looks smaller (4±1mm) in all the selected cultivars. Number of grains in per 100g was higher in desi cultivar BG-20211 (571±0.00) while lowest in kabuli variety BG-3022 (294±0.5). These findings were consistent with earlier studies that showed notable size disparities between desi and kabuli cultivars, mostly as a result of their distinct genetic backgrounds [20].
4.2 Proximate composition of different cultivars of Chickpea
Eight chickpea cultivars analyzed in 2019, comprising four desi (BG-3062, BG-20211, BG-1053, K-850) and four kabuli (BG-3022, BG-2024, BG-1103, BG-1108) types, underwent proximate analysis, with the results presented in Figure 2. The protein content ranged from 19.13% to 25.36%, with the highest value in cultivar K-850 (25.36% ± 1.83%), followed by BG-2024 (24.56% ± 1.88%). According to Pasha, Anjum, and Morris [21], a higher protein content is indicative of good quality functional food products. In this study, the ash content of chickpea flour ranged from 2.63% to 3.73%, with cultivar BG3022 exhibiting the highest ash content at 3.73% and cultivar BG-3062 showing the lowest at 2.63%. This variation in ash content may impact the overall quality and functionality of the chickpea flour. Ash is a measure of the mineral content in food, as it is what remains after water and organic acids are removed through heating. Minerals are not destroyed by heat because they have lower volatility compared to other components in food (22). The fat content of chickpeas ranged from 2.5% to 4.24%. The cultivar BG3022 had the highest fat content at 4.24±0.32%, while the BG3062 cultivar had the least fat content at 2.5±0.22%. Previous studies have reported varying fat content in Bengal gram, ranging from 2.05% to 7.42% [23, 24, and 25]. In contrast, the current study found that chickpea cultivars contain a moderate amount of fiber, ranging from 3.1% to 4.5%. The desi cultivar BG-3062 had the highest fiber content (4.5%), followed by K-850 (4.3%), and the lowest was in BG-1053 (3.1%). Additionally, the carbohydrate content of the chickpeas was substantial, ranging from 53.74% to 62.2%, making them a good source of energy. These findings highlight the nutritional value of chickpeas, with variations in fiber and carbohydrate content across different cultivars. The results showed that the cultivar BG1103 had the maximum carbohydrate content i.e. 62.2±0.32%, followed by BG3062 (59.87±0.13%). A comparison of established cultivars for their proximate composition over a span of ten years showed that the values for ash content were not affected by the cropping year. However, the protein content in desi cultivars (BG1103 and K850) increased by 7.2% and 10.2%, respectively, while it decreased by 18.93% and 30.84% in kabuli cultivars (BG-1053 and BG-1108), after a decade gap. The variation in protein content among chickpea cultivars may be attributed to environmental factors such as temperature, fertility, and soil conditions, as suggested by Zheng and Wang [26]. This is consistent with the findings of Pace et al. [27], who observed fluctuations in protein and nitrogen-free extract (NFE) content in sweet potatoes over a one-year period. These studies indicate that environmental influences can significantly impact the nutritional composition of crops, leading to variations in protein content and other nutritional components. Our study also observed similar results, noting that the location and genotypes greatly influenced all chickpea cultivars. Yegrem et al. [28] conducted study on chickpea and their analysis of eleven chickpea varieties showed moisture levels ranged from 10.85% to 12.06%, protein levels roved from 16.21% to 19.28%, crude fat levels roamed from 5.24% to 6.43%, carbohydrate levels arrayed in between 59.61% to 63.34%, ash levels outlined from 3.11% to 4.19%, and crude fiber levels traversed from 5.92% to 7.49%. In 2021, Yegrem [29] outlined the moisture levels ranging from 5.73% to 12.10%, ash levels from 2.47% to 3.87%, total lipid levels from 3.77% to 7.41%, and protein content ranged from 12.02% to 24.91% in different Ethiopian chickpea varieties. The nutritional compositions found in this study are comparable to existing values reported in previous research. Yegrem et al. [28] discovered significant variations in the nutritional composition of eleven chickpea varieties over a two-year period [2018-2019], suggesting that genetic factors contribute to these differences. Similar findings were reported by Yegrem et al. [29]. Additionally, some studies have noted slightly higher fat content in chickpeas [1, 28, 30, and 31]. These results indicate that both genetic and environmental factors can influence the nutritional composition of chickpeas, leading to variations in proximate values among different cultivars and growing conditions.
4.3 Non-nutrient composition of different Chickpea Cultivars
Dry legumes, such as chickpeas, are a vital food source and offer numerous health benefits due to their high content of polyphenols and flavonoids [32]. The bioactive compound content of the tested desi and kabuli chickpea genotypes is presented in Table 1. Notably, the total phenolic content (TPC) of chickpea varied from 101 to 276 mg Gallic Acid Equivalent (GAE) per 100g, highlighting the significant presence of phenolic compounds in these legumes. This range suggests that different chickpea genotypes may offer varying levels of antioxidant activity and potential health benefits. The highest TPC content was observed in BG3062 cultivars followed by K850, BG20211, BG1103, BG1108, BG3022, BG2024 and BG1053 respectively. TPC content in desi cultivar was significantly higher than kabuli cultivars, when the established cultivars were compared over a duration of 10 years the value remained almost similar to the previous values. de Camargo et al. [33] reported higher TPC content than our result (31.5-17.3 mg GAE/100g) while Johnson et al. [2] reported TPC content from 56.4-259.1 mgGAE/100g in the selected variety of chickpea. Ferreira et al. [1] reported free phenolic content 369.47 mg/100g while bound phenolic content 109.01 mg/100g in raw chickpea. Phenolic chemicals are of great importance since they contribute to the seed color, sensory features, and various biological traits. In this study desi cultivars with darker seed coat showed better phenolic content. TPC enables for the assessment of phenolic compound content or presence in a sample. Phenolic chemicals display redox properties that account for their antioxidant effects. These variations could be attributed to the grain type, being different varietals, harvest conditions, and extraction procedures. The Total Flavonoid Content (TFC) of chickpea cultivars varied from 0.100 to 0.173 mg/g, with the highest content found in cultivar BG-3062 (0.173±0.06 mg Quercetin Equivalent (QE)/g), followed by BG-20211 and K-850, respectively (Table 2). These values are comparable to those reported by Saleh et al. [34], who found
total phenols and total flavonoids in chickpeas to be 5.68 mg Gallic Acid Equivalent (GAE)/g and 8.43 mg Quercetin/g, respectively. The correlation between total phenolic content and seed color in chickpeas has been observed in numerous studies, suggesting that seed color may be an indicator of the antioxidant potential of chickpea grains. A slight decline in TFC content was recorded in 2019. The variation in TFC among cultivars may be attributed to genetic factors, environmental influences, or a combination of both. Analyzed chickpea genotypes are therapeutic functional foods since they have their abundance of bioactive chemicals, including phenolics and flavonoids, and high protein content. Chickpea seed flour can supplement a balanced diet and enhance functional foods.
Phytate content of the studied chickpea cultivars ranged from 579-891.6 mg/100g. Higher phytate content was found in BG-1103, K850 and BG-20211 followed by BG-3022 while lower amount of phytate was reported in BG-3062 and BG-1053. Sinkovic et al. [35] reported higher phytate content in chickpea 1116mg/100g DW, this outlined the comparatively lower phytate content in our experimented cultivars. Comparatively desi cultivars of chickpea except BG3022 exhibited more phytate content than kabuli. Cropping year showed considerable variation in amount of phytate content in all four cultivars. BG1103 (13.98%), K850 (9.83%) and BG1053 (5.4%) cultivars showed increase in phytate content whereas BG1108 showed decline (14.85%) in phytate content in the year 2019 in comparison to 2009 [36]. Phytic acid, a non-protein antinutrient, has the ability to complex micro- and macroelements, as well as reduce mineral and protein bio-functions and complex enzyme ion cofactors. Recent studies by Sinkovic et al. [35] and Upadhyay et al. [37] have highlighted the potential health benefits of small amounts of phytic acid, a compound often considered an antinutrient. Research has shown that moderate levels of phytic acid may offer several nutritional advantages, including lowering blood glucose levels, preventing dental cavities, reducing the risk of colon cancer, and exhibiting antioxidant activity. These findings suggest that phytic acid, in small quantities, may have beneficial effects on human health, challenging its traditional classification as a solely negative compound. This new understanding of phytic acid's role in nutrition highlights the importance of reevaluating its impact on human health and potentially harnessing its benefits. To minimize the loss of micronutrients, it is advised to consume 25 mg or less of phytate per 100 g of diet. However, the pulses examined in this study exhibited significantly higher levels of phytic acid, which can be reduced by soaking and boiling.
The impact of climate change was observed to be highest on phytate content. The established cultivars grown in year 2019 had significantly high content of phytate. In contrast, TPC content was not changed in all established cultivars. When we compared the important meteorological data of climate change of year 2009 and 2019, we found that important changes, max temperature was increased from 29.5 to 37. 4 in year 2019, an increase of about 8degrees during months of 1st Nov to 31st March. A maximum rainfall of 55.8 mm was recorded in month of Jan 2019 in contrast to the negligible rainfall in the year 2009. Similarly relative humidity was also less in year 2019 in comparison to 2009 (Supplementary material S1 and S2). Impact of specific environmental conditions on phytate content is not available however, it is known that phytate is the major storage of phosphorus in plants which deposits in aleurone layer of seeds during its maturity. It also participates in metabolic pathways of plant and acts as antioxidant, hence it is assumed that climate change particularly extreme temperatures (max and min) and untimely rainfall may be responsible for high phytate formation in chickpea grown in 2019.
4.4 Mineral Analysis of different chickpea cultivars
Chickpeas, like other plants, are a rich source of essential minerals such as iron, zinc, calcium, potassium, phosphorus, and magnesium, which are present in their edible parts like leaves and seeds [38]. However, the mineral content in chickpeas can vary significantly depending on factors like agricultural practices, genotype, and environmental conditions, as highlighted in previous studies [39, 40, and 41]. This variability emphasizes the importance of considering these factors to optimize mineral content in chickpeas. Table 2 represents the mineral profiling of selected varieties of different chickpea cultivars. Calcium content was found highest in BG20211 whereas phosphorus was highest in BG3022. The mean value of Calcium (Ca) in BG-20211 (142.13±0.13mg/100g) followed by BG-3022 and K850. The chromium content in the chickpea cultivars varied, with the highest value of 0.23 mg/100g found in BG-1108, followed closely by BG-3062 and BG-2024, which had similar values. In contrast, the lowest chromium content of 0.09 mg/100g was observed in the K-850 cultivar. This variation in chromium content highlights the differences in mineral composition among the chickpea cultivars. Copper content in chickpea was highest in BG-1108 followed by BG-1053. BG-1108 and BG-20211 are outstanding iron varieties showing higher iron content. Iron and copper are important in plant metabolism and nitrogen fixation. Potassium was highest observed in BG-1053 (1373.13±1.45 mg/100g). The magnesium content in the chickpea cultivars was found to be highest in BG-3062, with a mean value of 116.45 ± 0.24 mg/100g, followed closely by BG-1053. This indicates that BG-3062 is a rich source of magnesium, an essential mineral that plays a crucial role in various bodily functions including good heart health. The presence of magnesium in significant amounts in these chickpea cultivars makes them a nutritious addition to a healthy diet. Magnesium plays a crucial role in resistance to biotic and abiotic stress, contributing to the plant's adaptation strategies to the environment [42], especially in the context of climate change. The mean value of manganese (Mn) in BG-1108 was highest followed by BG-3022 and BG-1103. The phosphorus content ranged from 377.51 to 827.99 mg/100g. BG-3022 cultivar appeared having the highest phosphorus followed by K-850, while the lowest phosphorus content was determined in BG-3062. The BG-1053 contained highest value of sulphur followed by BG-3062. Zn content was high in kabuli type compared to desi type. Zinc had maximum value in BG-2024 (8.58±0.02mg/100g) while K-850 contained lowest zinc content among the studied cultivars. Yegrem et al, [28] reported Ca (164.31-211.67 mg/100g), Fe (5.86-6.73 mg/100g), Mg (107.54-123.90 mg/100g) and Zn (1.61-2.59 mg/100g) content in 11 chickpea varieties. This shows that Fe, Mg and Zn were comparatively higher in current study. Table 2 also shows that boron (B) content was highest in BG-20211 (2.40±0.00 mg/100g) followed by BG-1103 where it recorded as 2.03±0.01mg/100g. BG-1108 cultivar appeared having the lowest boron content (1.18±0.01 mg/g). Sharma et al. [36] performed the mineral analysis of four established cultivars in 2009 and reported that iron and zinc content were high in BG1103, K850 and BG1053, these values were decreased while iron and zinc were increased in BG1108 from previous values (2009). Zinc content in kabuli cultivars was higher than desi types. New chickpea cultivars BG2024 and BG3022 are identified as outstanding varieties with highest zinc content and appreciable amount of iron. The variations in the mineral profile of seeds can be attributed to diverse agricultural practices, soil types, the use of different fertilizers or pesticides/herbicides, as well as genetic factors.
Pulses are thought to be high in micronutrient. In India, there has been progressive drop in pulse consumption along with per capita availability of pulses in financial year 2023 (47.1g/day) from previous years against the recommended value of 60g/day (per capita requirement), despite an increase in mineral deficiencies [43]. Chickpea is a major legume consumed in India hence contributes to protein micromineral requirement of maximum vegetarian population, this emphasizes the need of judicious selection of variety. The new chickpea varieties were found to be rich in essential minerals such as calcium, iron, and zinc, which play vital roles in the body. This is particularly significant for populations in developing countries in North America, Europe, and South Asia, where deficiencies in these minerals are highly prevalent. The increased mineral content in these new varieties can help address these deficiencies, providing a nutritious solution for vulnerable populations. The enhanced levels of calcium, iron, and zinc can contribute to improved overall health, making these chickpea varieties a valuable resource for combating mineral deficiencies. A newly developed chickpea that is high in minerals shows great potential for helping populations with mineral deficiencies. By adding important minerals like iron, zinc, and calcium to a legume that is commonly eaten, it directly addresses nutritional gaps. This is especially important in areas where diets usually lack these nutrients, which can lead to many people having deficiencies and health problems related to them. For instance, iron is needed to make hemoglobin and prevent anemia, zinc is important for immune function and healing wounds, and calcium is essential for keeping bones healthy and preventing osteoporosis. Hence newly developed variety should introduced for consumption among population to address the deficiencies as well as promoted for cultivation.