Table 1A: splice donor site prediction in wildtype Spike ORF
Position pot. splice donor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
454-473 TGGATGGAAA•GTGAGTTCAG 0,187 +1
541-560 GGAAAACAGG•GTAATTTCAA 0,151 +1
894-911 GAAACAAAGT•GTACGTTGAA 0,565 +1
1323-1342 TGATTCTAAG•GTTGGTGGTA 0,357 0
1996-2015 ATTGGTGCAG•GTATATGCGC 0,707 +1
3296-3317 GCACACACTG•GTTTGTAACA 0,160 +2
–––––––––––––––––––––––––––––––––––––––––––––––––
Consensus MAG•GTNNGTG
Position pot. splice acceptor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
760-779 TCTTCTTCAG•GTTGGACAGC 0,667 +2
1183-1202 GTCTATGCAG•ATTCATTTGT 0,772 +2
1830-1849 TCTTTATCAG•GATGTTAACT 0,321 0
1906-1925 TATTCTACAG•GTTCTAATGT 0,226 +2
2815-2834 TCTTCCACAG•CAAGTGCACT 0,395 +2
–––––––––––––––––––––––––––––––––––––––––––––––––
Consensus YYYYYYNCAG•GGTN
Table 1B: splice donor site prediction in codon-optimized Spike ORF of AZ
Position pot. splice donor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
497-516 GCACCTTCGA•GTACGTGTCC 0,990 +2
563-582 ACCTGCGCGA•GTTCGTGTTT 0,200 +2
1175-1194 TCACAAACGT•GTACGCCGAC 0,187 +2
1209-1228 GGGAGATGAA•GTGCGGCAGA 0,162 0
1812-1831 CTCCAACCAG•GTGGCCGTGC 0,547 0
2331-2350 CACCCAAGAG•GTGTTCGCCC 0,202 +2
2949-2968 ACTGGACAAG•GTGGAAGCCG 0,378 0
2961-2980 GGAAGCCGAG•GTGCAGATCG 0,265 0
3083-3102 AGATGTCTGA•GTGTGTGCTG 0,516 +2
3296-3315 GCACCCATTG•GTTCGTGACC 0,357 +2
3452-3471 AACTGGATAA•GTACTTTAAG 0,296 +2
3555-3574 GCTGAACGAG•GTGGCCAAGA 0,309 0
3605-3624 AACTGGGGAA•GTACGAGCAG 0,813 +2
–––––––––––––––––––––––––––––––––––––––––––––––––
Position pot. splice acceptor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
170-189 CTTTCTTCAG•CAACGTGACC 0,299 +1
708-727 CCGGTTTCAG•ACACTGCTGG 0,201 0
804-823 CTACCTGCAG•CCTAGAACCT 0,276 0
1011-1030 CTTCGGCGAG•GTGTTCAATG 0,206 0
1561-1580 CCTGCCACAG•TGTGCGGCCC 0,265 +2
1910-1929 CCACCGGCAG•CAATGTGTTT 0,198 +1
1923-1942 TGTGTTTCAG•ACCAGAGCCG 0,260 0
2153-2172 TCACCATCAG•CGTGACCACA 0,192 +1
2676-2695 CGCTCTGCAG•ATCCCTTTGC 0,175 0
2694-2713 TGCTATGCAG•ATGGCCTACC 0,782 0
3205-3224 CCCGCTCAAG•AGAAGAATTT 0,287 +2
–––––––––––––––––––––––––––––––––––––––––––––––––
Table 1C: splice donor site prediction in codon-optimized Spike ORF of J&J
Position pot. splice donor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
563-582 ACCTGCGCGA•GTTCGTGTTT 0,150 +2
1175-1194 TCACAAACGT•GTACGCCGAC 0,180 +2
1209-1228 GGGAGATGAA•GTGCGGCAGA 0,207 0
1812-1831 CTCCAACCAG•GTGGCCGTGC 0,547 0
2331-2350 CACCCAAGAG•GTGTTCGCCC 0,202 +2
2949-2968 ACTGGACAAG•GTGGAAGCCG 0,378 0
2961-2980 GGAAGCCGAG•GTGCAGATCG 0,194 0
3083-3102 AGATGTCTGA•GTGTGTGCTG 0,495 +2
3296-3315 GCACCCATTG•GTTCGTGACC 0,392 +2
3452-3471 AACTGGATAA•GTACTTTAAG 0,436 +2
3555-3574 GCTGAACGAG•GTGGCCAAGA 0,335 0
–––––––––––––––––––––––––––––––––––––––––––––––––
Position pot. splice acceptor sites Score Type
–––––––––––––––––––––––––––––––––––––––––––––––––
708-727 CCGGTTTCAG•ACACTGCTGG 0,172 0
804-823 CTACCTGCAG•CCTAGAACCT 0,205 0
1011-1030 CTTCGGCGAG•GTGTTCAATG 0,207 0
1561-1580 CCTGCCACAG•TGTGCGGCCC 0,265 +2
1910-1929 CCACCGGCAG•CAATGTGTTT 0,194 +1
1923-1942 TGTGTTTCAG•ACCAGAGCCG 0,267 0
2153-2172 TCACCATCAG•CGTGACCACA 0,163 +1
2676-2695 CGCTCTGCAG•ATCCCTTTGC 0,175 0
2694-2713 TGCTATGCAG•ATGGCCTACC 0,782 0
3205-3224 CCCGCTCAAG•AGAAGAATTT 0,181 +2
–––––––––––––––––––––––––––––––––––––––––––––––––
ACE2 binding domain: encoded by nucleotides 998-1720 (R319-F551)