Peanut early leaf spot caused by Passalora arachidicola (Cercospora arachidicola), is a worldwide common fungal disease in peanut leaves, which occurs in all production areas in China. To obtain biocontrol resources to control peanut early leaf spot, 60 healthy peanut leaves were collected from major peanut production areas in Liaoning Province. A total of 563 strains were screened from these leaves. Eighteen strains showed differing levels of resistance against P. arachidicola. Among these strains, strain TL6 inhibited the pathogen most strongly, and the diameter of inhibition zone was 64.3 mm. This strain was able to inhibit 10 other types of pathogens. It was identified as Bacillus amyloliquefaciens based on its morphological characteristics, physiological and biochemical reactions and a comparative analysis of its 16S rDNA sequence. The fermentation liquor of strain TL6 was effective at controlling peanut early leaf spot, and the field control effect was above 69.17% after spraying the fermentation liquid of TL6. The field control effect was more than 40.96% after spraying the fermentation liquid diluted 200 times. The field control effect of the TL6 fermentation liquid diluted 200 times and including the addition of 500 gL-1 carbendazim diluted 1000 times inhibited P. arachidicola by 81.33%. The combination of TL6 and carbendazim had a significant synergistic effect. This strain of B. amyloliquefaciens shows promise for commercial development and application.