Prime editing is a versatile genome-editing technique. However, some genomic sites remain difficult to edit and optimal design of prime-editing tools is elusive. We present a fluorescent prime editing and enrichment reporter (fluoPEER), which can be tailored to any genomic target site. This system rapidly and faithfully ranked the efficiency of prime edit guide RNAs (pegRNAs) and any prime editor protein, including novel variants with flexible protospacer adjacent motif (PAM) recognition. We applied fluoPEER to instruct correction of pathogenic variants in patient cells and found that plasmid-editing enriched for editing at the genomic target site. Transcriptomic analysis of reporter-edited cells revealed that successful prime editing was associated with expression of DNA repair genes. Together, our results show that fluoPEER can be employed for rapid and efficient correction of patient cells, selection of gene-edited cells, and elucidation of cellular mechanisms needed for successful prime editing.