Therapeutic Potential of Human Induced Pluripotent Stem Cells and Renal Progenitor Cells in Experimental Chronic Kidney Disease

doi:10.21203/rs.3.rs-60474/v1

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Therapeutic Potential of Human Induced Pluripotent Stem Cells and Renal Progenitor Cells in Experimental Chronic Kidney Disease

https://doi.org/10.21203/rs.3.rs-60474/v1

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published 09 Dec, 2020

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Background

Chronic Kidney Disease (CKD) is a global public health problem. Regenerative medicine using pluripotent stem cells represents an attractive therapeutic approach for the treatment of CKD.

Methods

We transplanted Mitomycin C (MMC)-treated human induced pluripotent stem cells (hiPSCs) and renal progenitors cells (RPCs) into a CKD rat model system. The RPCs and hiPSCs cells were characterized by immunofluorescence and qRT-PCR. Untreated 5/6 nephrectomized rats were compared to CKD animals receiving the same amount of MMC-treated hiPSCs or RPCs. Renal function, histology and immunohistochemistry were evaluated 45 days post-surgery.

Results

We successfully generated hiPSCs from peripheral blood and differentiated them into RPCs expressing renal progenitor genes (PAX2, WT1, SIX2, and SALL1) and podocyte-related genes (SYNPO, NPHS1). RPCs also exhibited reduced OCT4 expression, confirming the loss of pluripotency. After cell transplantation into CKD rats, the body weight change was significantly increased in both hiPSC and RPC groups, in comparison with the control group. Creatinine clearance (CCr) was preserved only in the hiPSC group. Similarly, the number of macrophages in the kidneys of the hiPSC group reached a statistically significant reduction, when compared to control rats. Both treatments reduced positive staining for the marker α-smooth muscle actin. Histological features showed decreased tubulointerstitial damage (interstitial fibrosis and tubular atrophy) as well as a reduction in glomerulosclerosis in both iPSC and RPC groups.

Conclusions

In conclusion, we describe that both MMC-treated hiPSCs and RPCs exert beneficial effects in attenuating CKD progression. Both cell types were equally efficient to reduce histological damage and weight loss caused by CKD. hiPSCs seems to be more efficient than RPCs, possibly due to an anti-inflammatory mechanism triggered by hiPSCs. These results demonstrate that the use of MMC-treated hiPSCs and RPCs improve clinical and histological CKD parameters, avoided tumor formation, and therefore may be a promising cell therapy strategy for CKD.

Stem Cell & Developmental Cell Biology

Cell- and Tissue-Based Therapy

Chronic Kidney Disease

Pluripotent Stem Cells

Stem cells

The diagnosis of kidney diseases has improved over the years and being followed by a continuous increase in public and health professional awareness of Chronic Kidney Disease (CKD). In line with this, the economic burden for National Health Systems worldwide is growing, with some countries spending more than a half of their budget on renal replacement therapy for the 2% of CKD patients that evolve to kidney failure. Additionally, the excess of associated comorbidities significantly increases the whole cost relative to CKD treatment [1]. In such a scenario, the development of new alternative treatments, in addition to dialysis and transplantation, is urgently needed.

Regenerative medicine offers the use of pluripotent stem cells as an attractive therapeutic approach for the treatment of CKD in its different stages. In this context, we have previously demonstrated that injection of bone marrow-derived mesenchymal stem cells (BM-MSCs) from healthy rats in an experimental model of CKD had beneficial effects [2]. However, a major drawback of using BM-MSCs derived from uremic patients is that those cells did not convey functional protection, suggesting that autologous BM-MSCs are not suitable for CKD treatment [3]. Thus, the use of induced pluripotent stem cells (iPSCs) and renal progenitor cells (RPCs) may be a more efficient approach.

Induced pluripotent stem cells constitute a newly defined stem cell type with similar properties to those displayed by embryonic stem cells (ESCs), in terms of self-renewal and differentiation [4]. Additionally, iPSCs have a wide differentiation capacity without raising ethical conflicts, such as those observed with ESCs. However, because of the iPSC pluripotent nature, tumor formation has been reported after cell transplantation, restricting their use (in an immature stage) for cell therapy [2,5]. Hence, blocking the proliferative ability of iPSCs with antimitotic agents, such as mitomycin C (MMC) and/or differentiating them into RPCs might be a safer strategy for CKD treatment [6-8].

In the present study we sought the effects of hiPSCs and RPCs on the experimental CKD. To that end, we first generated hiPSCs and differentiated them into RPCs, and then we tested whether both cell types could be effective as potential therapeutic agents in a 5/6 CKD rat model.

Reprogramming of PBMCs into pluripotent stem cells

Using the protocol described by Okita et al. [9], we derived hiPSCs from peripheral blood mononuclear cells (PBMCs). Epissomal vectors (pCE-hOct3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, and pCXB-EBNA1) obtained from the Addgene plasmid repository were used for reprogramming.

PBMCs were collected from healthy donor in the presence of anticoagulant agents and then purified by density gradient centrifugation with Ficoll-paque Plus (GE Healthcare, density 1077 g/L) according to the manufacturer's instructions. These isolated cells were cultured in X-VIVO-10 medium (Lonza) containing 100 ng/mL Erythropoietin (Peprotech), 10 ng/mL IL-3 (Peprotech), 50 ng/mL IGF-1, and 100 ng/mL stem cell factor (SCF; R&D Systems) for hematopoietic progenitor expansion. A mixture containing 1 μg of each plasmid was used for nucleofection of 1×10⁶ hematopoietic progenitors using the Nucleofector 4D (Lonza) device with a P3 Nucleofector kit according to the manufacturer's instructions. ReproTeSR medium was added 48h and 96h after nucleofection, and then changed every 48h until reprogrammed colonies appeared (20-30 days after nucleofection). The colonies were manually passed and cultured in Matrigel^R-treated plates in mTeSR-1 medium (StemCell Tech, Vancouver, BC, Canada).

To reduce the cell proliferation rate and the ability of hiPSCs to generate tumors, these cells were treated with mitomycin C (MMC), an antibiotic which is capable of arresting cell proliferation [6,7], 24 h before transplantation. MMC (10 μg/mL) was added to the culture medium for 3 h, before fresh medium addition [10].

hiPSCs differentiation into renal progenitor cells

The differentiation protocol followed was previously described by Song et al. [8]. The hiPSCs colonies were mechanically fragmented into pieces of similar size. The fragments obtained were then transferred to ultra-low attachment six-well plates (Corning Costar) and maintained in culture for 72h in differentiation medium, which consisted of DMEM F-12 (50/50%) (Gibco), 2.5% fetal bovine serum (FBS; Gibco), 0.1 mM non-essential aminoacids, 0.1 mM beta-mercaptoethanol (Gibco), 1% antiobiotic-antimycotic (Gibco), and was supplemented with the following nephrogenic factors: 0.1 μM retinoic acid (RA) (Tocris), 10 ng/mL activin A (Pepro Tech), and 15 ng/mL BMP7 (Prepotech).

After 72h in ultra-low attachment plates, the embryoid bodies (EBs) generated were transferred to culture plates coated with 0.1% gelatin (Sigma), with cells adhering and proliferating in differentiation medium for another 7-8 days. The cells were then seeded using the basal medium, without nephrogenic factors, until their characterization. During the entire culture process, the medium was changed every other day and cells were expanded with trypsin.

Immunofluorescence staining

For immunofluorescence staining, hiPSCs colonies and RPCs were fixed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1h at room temperature. hiPSCs colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scientific, 10 μg/mL), and anti-Sinaptopodin (Thermo Scientific, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scientific, 10 μg/mL), and Sinaptopodin (Thermo Scientific, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scientific, Waltham, MA, USA).

RNA isolation from culture cells and quantitative real-time PCR (qRT-PCR)

RNA from erythroblasts, hiPSCs, EBs, and RPCs was extracted using the Illustra RNAspin Mini Kit (GE Healthcare) method, following the manufacturer’s protocol. The RNA was transcribed into complementary DNA (cDNA) using 1 μg of total RNA and High-Capacity cDNA Reverse Transcription kit (Applied Biosystems), following the manufacturer´s instructions.

Quantitative real-time PCR was carried out using TaqMan Gene expression Master Mix (Applied Biosystems). Primers to the following genes were used for hiPSCs and RPCs expression analysis: PAX2, SIX2, WT1, SALL1, NPHS1, SYNPO, OCT4, NANOG, ACTB, GAPDH and HMBS. The primers used in the amplification reactions are shown in additional file (see Additional table 1). The threshold cycle (Ct) values were measured in triplicate and normalized against the endogenous controls (ACTB, GAPDH and HMBS). Erythroblasts or normal human kidney cells served as reference controls.

In Vivo Experiments

This study was carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The experimental protocol was previously approved by the Ethics Committee of FAMERP Medical School (São Jose do Rio Preto, Brazil - Permit Number: 001-001788/2017). All surgeries were performed under anesthesia and all efforts were made to minimize animal suffering. Overdose anesthetic (sodium pentobarbital) was the euthanasia method in this study.

Thirty-two male Wistar rats (250-300 g) were maintained under a 12h light/dark cycle, with food and water available ad libitum. The 5/6 reduction of renal mass was performed in accordance with previously described techniques [11]. Briefly, male rats were anaesthetized with xylazine (10 mg/kg) and ketamine hydrochloride (50 mg/kg). Trichotomy and antisepsis of the ventral abdominal area were conducted, and animals were positioned on horizontal dorsal decubitus. Subtotal renal ablation was carried out in a surgical procedure through a right nephrectomy and selective infarction of two thirds of the left kidney by the ligation of extrarenal branches of the left renal artery, remaining approximately one-sixth of the total kidney tissue mass without lesions.

Experimental design

The rats were divided into the following groups: CKD (n=8), which received only cell culture medium after the surgery, RPC (n=8), which received 1×10⁶ RPCs, (n=8), hiPSC (n=8) which received 1×10⁶ MMC-treated hiPSCs, and sham-operated animals (n=8).

Immediately after mass reduction and right nephrectomy, hiPSCs, RPCs, or culture medium were injected into the renal parenchyma, specifically, between the infarcted and the healthy areas. The abdominal cavity was then sutured and all animals were observed for 45 days.

Renal function analysis

To collect 24h urine, all animals were weighed and allocated to metabolic cages (Tecniplast, Buguggiate-VA, Italy) at baseline and 45 days after surgery. Also, during the surgery and immediately before the euthanasia, blood was collected from the cava vein. Renal function was evaluated by the dosages of serum creatinine (SCr), creatinine clearance (CCr), rate of decline of CCr (RCCr mL/min/day) and 24h proteinuria (PT-24h). The RCCr mL/min/day was used as a measure of CKD progression. After 45 days of surgery, all animals were euthanized, and the kidneys were processed for histological and immunohistochemistry analysis.

A Colorimetric assay (Jaffe reaction, spectrophotometer BTS 310; Biosystems S.A., Barcelona, Spain) was used to measure plasma and urine SCr and urine protein concentrations. Creatinine clearance and the rate of decline of CCr were calculated from the dosages of serum and urinary SCr levels. Blood pressure was evaluated by an indirect tail-cuff method (Insight LTDA, Ribeirão Preto, SP, Brazil), and the average of three measurements was used for analysis.

Histological and immunohistochemical analyses

For histological evaluation, the renal tissue was fixed in formalin and embedded in paraffin. The sections were stained with hematoxylin and eosin (HE) and Masson's trichrome. A semi-quantitative score was derived for each sample, to determine the extent of tubule-interstitial changes, as previously described [12]. The interstitial fibrosis and tubular atrophy (IFTA) score was obtained according to the appropriate proportion of tissue affected (0 to 100%). For glomeruloesclerosis analysis, the number of affected glomeruli was counted and divided by the total glomeruli number. All sections were evaluated by a blind observer.

Immunohistochemical (IHC) staining was performed as previously described [13]. The tissue sections were incubated using the following primary antibodies: CD68 (Serotec, MCA1957GA, 1:250), anti-human nucleoli antigen antibody [NM95] (Abcam, ab190710, 1:500) and α-smooth muscle actin (α-SMA) (Dako, clone 1A4, cat. M0851, 1:100). All primary antibodies were incubated overnight at 4°C and an IHC detection kit was used (Abcam, Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)). For evaluation of immunoperoxidase staining for a-SMA, each grid field was semi-quantitatively graded and the mean score per kidney was calculated. Each score reflected mainly changes in the extent, rather than the intensity, of staining and depended upon the percentage of grid field showing positive staining: 0=absent or less than 5%; I=5%–25%; II=25%–50%; III=50%–75%; IV>75%. The number of CD68-positive cells in each section was calculated by counting the number of positive cells in 30 sequential grid fields (0.245-mm²) from the renal cortex [13].

Statistical analysis

Analyses were performed using StatsDirect version 3.0 software (StatsDirect Ltd, Cheshire, UK), with the critical level set at p<0.05. Data were expressed as mean ± standard deviation. Comparisons among multiple groups were performed using analysis of variance (ANOVA). When p values were significant, differences between the groups were specified using Tukey’s post hoc multiple comparison test. When comparing data, the two-sided Student’s t-test and Mann-Whitney U tests were used.

Generation and characterization of hiPSCs

Approximately 20-25 days after the nucleofection process, it was possible to observe the formation of hiPSCs colonies (Figure 1). The cells showed pluripotent stem cell morphology, a high nucleus/cytoplasm ratio, refringent colonies, and a high proliferative rate in culture.

To further characterize the generated hiPSCs, we used immunofluorescence and qRT-PCR analysis. The colonies displayed positive staining for pluripotency markers, as well as a high expression of OCT4 and Nanog (Figure 1), demonstrating their pluripotent nature.

Figure 1. Characterization of peripheral blood derived hiPS cells. (A) Typical morphology of undifferentiated hiPSCs colonies. The colonies grown over Matrigel have a homogeneous shape, smooth and regular edges and positive staining for (B) OCT4, (D) NANOG (C) and (E) DAPI staining for the same colonies of (B) and (D), respectively. Bar: 200um. (F) Gene expression levels in samples collected before (Erythroblasts) and after (hiPSCs) reprogramming, generated by referencing each gene to HMBS expression levels as an internal control. Healthy erythroblasts mRNA was used as the comparative sample. *p-Value <0.05 vs. Erythroblast.

Differentiation of hiPSCs into RPCs

The hiPSC differentiation process consisted of a 10-day protocol, involving a combination of RA, activin A, and BMP7. The process began with the manual fragmentation of hiPSC colonies into pieces of similar sizes (Figure 2A, 2B). Approximately 24h later, it was possible to observe fragments aggregation, forming non-adherent cell clusters, i.e., embryoid bodies (Figure 2C, 2D).

Figure 2. Mechanical fragmentation and formation of embryoid bodies (EB). (A, B) The hiPSC colonies were mechanically fragmented in approximately similar sizes. (A) Magnification of 20x and (B) magnification of 40x. (C) The fragments were collected and began to aggregate, magnification of 40x. (D) After 24h, the embryoid bodies were already formed, magnification of 20x.

After adhesion onto gelatin-coated plates, in the continuous presence of the differentiation medium, cells began to undergo morphological changes. At early stages, cells appeared as single cells, but throughout the differentiation process, their morphology became increasingly similar to renal podocytes. In the final days of the differentiation process, it was possible to observe cytoplasmic extensions with an arborized appearance.

hiPSCs and RPC characterization

The hiPSCs and RPCs were characterized by immunofluorescence and qRT-PCR analysis. On day zero of the differentiation process, the hiPSCs were negative for immunostaining with PAX2, WT1, and Nephrin, and positive for the Synaptopodin podocyte marker (Figure 3). After 12 days, PAX2, WT-1, Nephrin, and Synaptopodin staining confirmed the successful differentiation of the hiPSCs into RPCs. The differentiated cells showed positive staining for PAX2 and WT1 (renal progenitor markers), and for Nephrin and Synaptopodin (podocyte markers), indicating their successful differentiation into renal podocyte progenitors (Figure 3). A total of 97% of the differentiated cells presented a positive immunostaining for RPC markers.

Figure 3.Immunofluorescence staining of cells during differentiation process of hiPSC into podocyte-like cells. (A) RPCs were positive for renal progenitors markers (PAX2 and WT1, nuclear and cytoplasmic, respectively) and renal podocytes markers (nephrin and synaptopodin, cytoplasmic) on day 12. Magnification 40X. (B) hiPSCs were negative for PAX2, WT1, and nephrin, and positive for synaptopodin on day 0. Magnification 20X.

The qRT-PCR results, shown in Figure 4, indicate the increased expression of renal progenitor genes (PAX2, WT1, SIX2, and SALL 1) during the initial phase of the differentiation process, and increased expression of renal podocyte genes (NPHS1 and SYNPO) after 10 days of the differentiation process. When compared to the undifferentiated hiPSCs and/or RPCs, the EBs showed an increased expression of PAX2, WT1, SIX2, and SALL1. After the differentiation process, RPCs displayed increased expression of the NPHS1 and SYNPO genes, when compared to hiPSCs and EBs. hiPSCs showed a decreased expression of the NPHS1 and SYNPO genes. Decreased expression of OCT4 was also observed throughout the differentiation process (Figure 4).

Figure 4. Quantitative real time PCR for RPC (day 16), EB (Day 3), hiPSC (day 0). EBs showed an increased expression of (A) PAX2 (p=0.001 vs. RPC, p=0.002 vs. hiPSC), (B) WT1 (p=0.03 vs. RPC, p=0.002 vs. hiPSC), (C) SIX2 (p=0.0001 vs. RPC, p=0.0001 vs. hiPSC), and (D) SALL1 (p=0.05 vs. RPC, p=0.01 vs. hiPSC). hiPSCs showed a decreased expression of (E) NPHS1 (p=0.001 vs. Human Kidney) and (F) SYNPO (p=0.03 vs. RPC, p=0.01 vs. EB, p=0.0002 vs. Human Kidney) on day 0, whereas the expression of both NPSH1 (E) and SYNPO (F) increased in RPCs on day 16. The pluripotency marker gene (G) OCT4 had a decreased expression during the differentiation process (p=0.04 hiPSC vs. EB, p=0.02 hiPSC vs. RPC). *p-Value <0.05.

Effect of hiPSCs and RPCs on clinical and histological parameters

As shown in Table 1, the body weight change was significantly lower in the CKD group, when compared to sham rats. In contrast, the body weight change in the treated groups was significantly increased when compared to the CKD group, similarly to the weight change observed in the sham group.

Table 1. Renal function parameter measurements at the end of the study (day 45).

Parameters	Groups
	Sham	CKD	RPC	hiPSC
Body weight change (g)	61.6 ± 36.2^a	-23.6 ± 101^b,c	56.7 ± 23	59.7 ± 14
Mean arterial pressure (mmHg)	127 ± 1	201 ± 23	172 ± 30	177 ± 37
CCr (mL/min)	1 ± 0.1	0.85 ± 0.1^d	0.91 ± 0.5^e	1.61 ± 0.5
RCCr (mL/min/d)	0.001 ± 0.003	0.01 ± 0.01	0.005 ± 0.01	0.002 ± 0.008
SCr (mg/dL)	0.6 ± 0.1^f	1.06 ± 0.5	0.87 ± 0.24	0.85 ± 0.13
PT24h (mg/24h)	1 ± 0.2^g	44.5 ± 22.6	45.8 ± 30	36 ± 21

Results are presented as mean ± SD. Abbreviations are: SCr= serum creatinine; RCCr= rate of decline of CCr; PT24h= 24-hour proteinuria; CCr= creatinine clearance, CKD, chronic kidney disease; RPC, renal progenitor cell; hiPSC, induced pluripotent stem cells.^ap < 0.05 vs. CKD; ^b,cp < 0.05 vs. RPC and hiPSC; ^dp < 0.05 vs. hiPSC;^ep < 0.05 vs. hiPSC, CKD;^fp < 0.01 vs. CKD; ^gp < 0.01 vs. CKD vs. RPC vs. hiPSC.

The mean arterial blood pressure was numerically reduced in the treated groups when compared to the CKD group (CKD= 201 ± 23.4 mmHg vs. RPC= 172 ± 30 mmHg vs. hiPSC= 177 ± 37 mmHg), but the difference did not reach statistical significance.

Renal functional studies

Only the treatment with hiPSC significantly preserved the creatinine clearance, when compared with the CKD and RPC groups (CKD= 0.85 ± 0.1 mL/min vs. RPC= 0.91 ± 0.5 mL/min/day vs. hiPSC= 1.61 ± 0.5 mL/min/day, p < 0.05). Although not statistically significant, treatment with both RPCs and hiPSCs slowed the RCCr by 50% and 80%, respectively, when compared to CKD rats (CKD= 0.01± 0.01 mL/min/day vs. RPC= 0.005 ± 0.01 mL/min/day vs. hiPSC= 0.002 ± 0.008 mL/min/day, p= 0.4). SCr was partially reduced by both cell treatments, while PT-24h remained unchanged during the observation period (Table 1).

Histological and immunohistochemical analyses

Histological analysis showed that kidneys from hiPSC and RPC groups exhibited less tubulointerstitial damage and less glomerulosclerosis, measured by lower IFTA scores, and better preservation of glomerular structures, when compared to CKD group (Table 2).

Table 2. Histological changes and the effect of cells treatment in the 5/6 nephrectomized animals.

	CKD	RPC	hiPSC
IFTA	44.9 ± 26^a,b	15.8 ± 13.2	13.6 ± 13.1
Glomerulosclerosis	0.13 ± 0,13^c,d	0.01 ± 0.01	0.008 ± 0.01

Data are expressed as means ± SD. (^ap= 0.003 vs. RPC; ^bp= 0.01 vs. hiPSC; ^cp= 0.01 vs. RPC;^dp =0.01 vs. hiPSC).

The immunohistochemistry analysis showed that the hiPSC group had a reduced number of macrophages in renal tissue, as showed by a greater decrease in the positive staining for CD68, when compared to the CKD group (CKD= 135 ± 32 vs. hiPSC= 27.9 ± 18, p < 0.001). Both cell types significantly reduced the positive staining for α-SMA (CKD= 2.46 ± 0.3 vs. RPC= 1.3 ± 0.4 vs hiPSC= 1.79 ± 0.7, p= 0.0006) suggesting a reduction in fibrosis (Figure 5).

Figure 5.Immunohistochemistry and quantitative analysis for Anti-CD68 and Anti-αSMA. Anti-CD68 for (A) CKD group, (B) RPC group and (C) hiPSC group, magnification of 40X. (D) Anti-CD68 quantitative analysis for all the three groups. Anti-αSMA for (E) CKD group, (F) RPC group and (G) hiPSC group, magnification of 40X. (H) Anti-αSMA quantitative analysis for all the three groups. *p-Value <0.05 vs. CKD.

In order to track the human transplanted cells into the rat renal tissue, we used an anti-human nucleoli antigen antibody. However, we could not track neither the hiPSCs nor the RPCs integration into the renal tissue after 45 days of transplantation. (Additional figure 1).

The prevalence of CKD has been rapidly increasing and the treatment of its comorbidities, dietary restriction, such as glycemic restriction, and pressure control, do not assure the cessation of the disease progression. Progression of CKD to its end stage leads to treatments that include dialysis and/or renal transplantation [1,14]. Dialysis is quite complex, reducing quality of life in patients, whereas renal transplantation, despite being a definitive therapy, is hampered by organ supply and shortcomings associated with immunosuppressive drugs [14]. For all these reasons, regenerative medicine has emerged as a promising alternative for CKD treatment [15]. However, it is also noteworthy that only few studies have currently addressed the use of cell therapy in CKD, limiting the possibility of reaching definitive conclusions [3,16-18].

In previous studies, we tested the efficacy of different types of stem cells in the 5/6 experimental CKD model and found that the cell therapy had the potential to retard the progression of CKD [2]. In the present study, we sought the efficacy of generated hiPSCs and RPCs in the same model but differently from other reports, we treated hiPSCs with MMC before differentiating them into RPCs, in order to avoid tumor formation [6, 19-24]. Using a nephrogenic cocktail composed of inducing factors [8, 25-27], we successfully differentiate hiPSCs into RPCs, which presented morphological characteristics and molecular markers typical of the podocyte lineage. Since podocytes injury or depletion may lead to dysfunction and alterations of the glomerular filtration barrier, and given that these cells cannot be naturally replaced after injury [28, 29], we hypothesized that, in vitro differentiation of hiPSCs into podocyte progenitor cells, could be a potential new approach to improve renal function at the end stage of CKD.

We observed that the body weight loss were reversed in both treated groups, indicating that iPSCs and RPCs could minimize the impact of CKD on general symptoms, allowing animals to feed regularly, thereby avoiding weight loss, described in CKD [30]. Increased arterial blood pressure levels, another hallmark of CKD, was also partially improved by RPCs and hiPSCs treatment, suggesting that both cell types may have contributed to ameliorating some pathophysiological mechanisms underlying hypertension in CKD [31, 32].

Interestingly, only hiPSC transplantation significantly improved creatinine clearance in comparison with the CKD and RPC groups. A similar improvement in renal function was also described by some authors using iPSCs and hiPSC-derived renal progenitors [33, 34]. The mechanisms underlying this selective beneficial effect of hiPSCs over the RPC could be due to the release of cytokines and renoprotective factors released by this cell type, as previously reported [33, 35]. Both iPSC and RPCs reduced the histological damage observed in CKD. The reduced renal fibrosis was corroborated by a decreased expression of α-SMA staining in the treated groups, suggesting that both cell treatments were able to reduce the fibrosis associated with end stage kidney disease. In addition, we demonstrated that the hiPSC treatment decreased macrophage CD68+ cells in the renal parenchyma, possibly due to a paracrine anti-inflammatory effect [33, 35]. Previous studies lend support to the present observation since it has been reported that mouse kidney progenitor-like cells reduce interstitial fibrosis, glomerular sclerosis, recruitment of macrophages and myofibroblasts in the interstitium, as well as the blood pressure levels in 5/6 CKD model [18].

Some mechanisms have been proposed to explain the beneficial effect of cell therapy on improving renal injury, especially with the use of iPSCs and RPCs [2, 17,18,22, 33-39]. RPCs can differentiate in vivo by forming renal tubules and acting through pro-proliferative, anti-apoptotic and anti-inflammatory effects [22], as well as by integrating into the kidney [17], decreasing the level of vascular rarefaction and preventing endothelial mesenchymal transition [18]. We were not able to localize neither the hiPSCs nor the RPCs integration into the renal tissue, after 45 days of transplantation, corroborating the findings described by Chen et al [18], which have observed only a few RPCs in the injured kidney as early as 28 days after cell transplantation. Thus, our results suggest that both hiPSC and RPCs may attenuate the CKD damage through paracrine effects. Most importantly, in the present study, we did not observe any tumor formation, neither with the use of hiPSCs nor with RPCs, indicating that the MMC-treated cells approach may be a safer strategy as cell therapy for CKD.

We do acknowledge that a major limitation of our study is the fact that the direct differentiation of hiPSCs into RPCs results in podocyte-like cells in a mature stage, probably limiting their full therapeutic potential. Another limitation is that the mechanisms proposed to explain the beneficial effect of both cell therapies are multifaceted and further studies are required to clarify the exact mechanisms related to the therapeutic effects of hiPSCs and RPCs.

In conclusion, our study provides evidence that the transplantation of hiPSCs and RPCs may be an effective therapeutic strategy for end stages of CKD. We also show that by using the MMC-treated hiPSCs approach we could reduce CKD damage in the absence of tumors generation. The treatment with both hiPSCs and RPCs reduces weight loss caused by CKD, improves renal function, as well as reduces fibrosis and glomerulosclerosis. These data represent a promising and safe strategy for CKD treatment, but further studies are necessary to corroborate its therapeutic benefits.

CKD: Chronic kidney disease

MMC: Mitomycin C

hiPSCs: Human induced pluripotent stem cells

RPCs: Renal progenitors cells

CCr: Creatinine clearance

BM-MSCs: Bone marrow derived mesenchymal stem cells

iPSCs: Induced pluripotent stem cells

ESCs: Embryonic stem cells

PBMCs: Peripheral blood mononuclear cells

SCF: Stem cell factor

FBS: Fetal bovine serum

RA: Retinoic acid

EBs: Embryoid bodies

BSA: Bovine serum albumin

cDNA: Complementary DNA

Ct: Threshold cycle

SCr: Serum creatinine

RCCr: Rate of decline of CCr

PT-24h: 24h proteinuria

HE: Hematoxylin and eosin

IFTA: Interstitial fibrosis and tubular atrophy

IHC: Immunohistochemical

α-SMA: α-smooth muscle actin

ANOVA: Analysis of variance

eGFR: Estimated glomerular filtration rate

hNPCs: Human nephron progenitors cells

Ethics approval and consent to participate

This study was carried out after approval by the Ethics Committee of FAMERP Medical School (São Jose do Rio Preto, Brazil - Permit Number: 001-001788/2017).

Consent for publication

Not applicable.

Availability of data and materials

The authors declare that the dataset supporting the conclusions of this study is included within the article and its supplementary information files.

Competing interests

The authors declare no competing interests.

Funding

This study was supported by São Paulo State Research Foundation (FAPESP – Fundação de Amparo à Pesquisa do Estado de São Paulo) grant number 2016/18586-0, and Coordination for the Improvement of Higher Education Personnel (CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), as a PhD fellowship to Patrícia de Carvalho Ribeiro.

Authors’ contributions:

PCR: Collection and/or assembly of data, data analysis and interpretation, manuscript writing;

FHL: Conception and design, collection and/or assembly of data, manuscript writing;

IMMFC, SAA, MASFB, GEFM: Collection and/or assembly of data;

MCS: Data analysis and interpretation, manuscript writing;

HCC: Conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript;

MAF: Conception and design, final approval of manuscript.

Acknowledgments

The authors would like to thank the Sao Paulo State Research Foundation (FAPESP) and the Coordination for the Improvement of Higher Education Personnel (CAPES) for the financial support.

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Therapeutic Potential of Human Induced Pluripotent Stem Cells and Renal Progenitor Cells in Experimental Chronic Kidney Disease

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