Understanding ecosystem services (ESs) and their interactions will help to formulate effective and sustainable land use management programs.This paper evaluates the water yield (WY), soil conservation (SC), carbon storage (CS) and habitat quality (HQ), taking the Yellow River Basin as the research object, by adopting the InVEST (Integrated Valuation of Ecosystem Services and Trade Offs) model. The Net Primary Productivity (NPP) was evaluated by CASA (Carnegie-Ames-Stanford approach) model, and the spatial distribution map of five ESs were drawn, the correlation and bivariate spatial correlation were used to analyze the trade-off synergy relationships between the five ESs and express them spatially. The results show that NPP and HQ, CS and WY are trade-offs relationship, and other ecosystem services are synergistic. The trade-off synergy shows obvious spatial heterogeneity. Driven by different factors, the leading ecological function services in the Yellow River Basin can be divided into three areas, and WY and SC service leading functional areas are mainly distributed in HQ and CS service leading functional areas and NPP service leading functional areas.The results of functional bundles are obviously affected by natural conditions such as land use/cover types and climate in the Yellow River Basin, which can provide the basis for the Yellow River Basin to regulate ESs and maximize benefits.