A Prospective Longitudinal Study Evaluating The Influence of Immunosuppressives and Other Factors On COVID-19 in Autoimmune Rheumatic Diseases

doi:10.21203/rs.3.rs-805748/v1

Background

We conducted this study to identify the influence of prolonged use of hydroxychloroquine(HCQ), glucocorticoids (GC) and other immunosuppressants (IS) on occurrence and outcome of COVID-19 in patients with autoimmune rheumatic diseases (AIRDs).

Methods

This was a prospective, multicenter, non-interventional longitudinal study across 15 specialist rheumatology centers. Consecutive AIRD patients on treatment with immunosuppressants were recruited and followed up longitudinally to assess parameters contributing to development of COVID-19 and its outcome.

Results

COVID-19 occurred in 314(3.45%) of 9212 AIRD patients during a median follow up of 177 (IQR 129,219) days. Long term HCQ use had no major impact on the occurrence or the outcome of COVID-19. Glucocorticoids in moderate dose (7.5-20mg/day) conferred higher risk (RR = 1.72) of infection. Among the IS, Mycophenolate mofetil (MMF), Cyclophosphamide (CYC) and Rituximab (RTX) use was higher in patients with COVID 19. However, the conventional risk factors such as male sex (RR = 1.51), coexistent diabetes mellitus (RR = 1.64), pre-existing lung disease (RR = 2.01) and smoking (RR = 3.32) were the major contributing risk factors for COVID-19. Thirteen patients (4.14%) died, the strongest risk factor being pre-existing lung disease (RR = 6.36, p = 0.01). Incidence ((17.5 vs 5.3 per 1 lakh (Karnataka) and 25.3 vs 7.9 per 1 lakh(Kerala) ) and case fatality (4.1% vs 1.3 % (Karnataka) and 4.3% vs 0.4% (Kerala)) rate of COVID-19 was significantly higher (p < 0.001) compared to the general population of the corresponding geographic region.

Conclusions

Immunosuppressants have a differential impact on the risk of COVID-19 occurrence in AIRD patients. Older age, males, smokers, hypertensive, diabetic and underlying lung disease contributed to higher risk. The incidence rate and the case fatality rate in AIRD patients is much higher than that in the general population.

Rheumatology

Autoimmune rheumatic diseases

SARS-CoV-2 infection

risk factors

outcome

hydroxychloroquine

glucocorticoid

immunosuppressants.

Long term HCQ use had no major impact on the occurrence or the outcome of COVID-19 in AIRD.
Glucocorticoids in moderate doses increased the risk of COVID-19 in AIRD patients.
The incidence rate of COVID-19 is at least 3 fold higher and the case fatality rate is 4.6 times higher than that of the general population in the same geographic area.
The risk of contracting COVID-19 is higher in AIRD patients, who are elderly, male, smokers, hypertensive, diabetic or with an underlying lung disease.

The coronavirus disease (COVID-19) pandemic has affected more than 131 million persons and led to over 2.8 million deaths across the world as of 4th April 2021(1). In the early part of the COVID 19 pandemic in China, it became clear that comorbidities such as diabetes (DM), hypertension (HTN), and advanced age are associated with poor outcome. (2) Patients with Autoimmune Rheumatic Disease (AIRD) have underlying immune dysfunction in addition to frequent use of glucocorticoids (GC) and other immunosuppressant (IS) medications. Hence it becomes imperative to identify the risk factors associated with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS- COV2) infection and outcome in AIRD.

In addition, with the better understanding of the molecular pathways and cytokine networks in SARS-CoV2 infection, the repurposing of the existing immunomodulatory drugs to curtail the hyperinflammatory state came to the forefront(3, 4). Early on in the pandemic, hydroxychloroquine (HCQ) was acclaimed as both a preventive and therapeutic treatment for COVID-19, but subsequent clinical trials have not found any benefit. Several immunomodulatory medications including glucocorticoids and biologicals, which may potentially inhibit one or more steps of the coronavirus life cycle or can counteract the amplified immune response were focused on amongst many others.(5)

AutoImmune Rheumatic Disease(AIRD) such as Rheumatoid arthritis (RA), Systemic lupus erythematosus (SLE), Systemic Vasculitis and several others are conventionally treated with HCQ in addition to other IS and GC. Dexamethasone has been evaluated in several clinical trials and was associated with a lower risk of mortality at 28 days in critically ill COVID-19 patients, compared to placebo or standard of care.(6) GCs form an important component of treatment for many of the AIRDs. Clinical trial protocols testing these molecules would normally exclude immunosuppressed patients.(7) Therefore, it is of significance to understand both the incidence and outcome of COVID-19 in AIRD who are already on treatment with these groups of medications.

The purpose of our study was three-fold. Firstly, through this prospective longitudinal study, we examined if the AIRD population already on long term HCQ treatment had any impact on occurrence or severity of COVID-19 than the general population. Secondly, we examined if the current use of GC and or IS would influence occurrence of COVID-19, or alter the intensity of associated hyperinflammation thereby reducing its severity in AIRD patients. Thirdly, to delineate the risk factors associated with occurrence and adverse outcome of COVID-19 in the AIRD cohort in our geographic region who were being followed up longitudinally during the 1st wave in India.

As we write this manuscript, India is experiencing a second wave of COVID-19 and in the foreseeable future it is likely to persist. Hence, it also becomes imperative that real-world data should define and help derive recommendations for COVID-19 in AIRD patients.

Study Design

This is a prospective, multicenter, non-interventional, longitudinal study involving 14 specialist rheumatology centers across Karnataka and one center in Kerala, India. Consecutive patients diagnosed with any of the AIRDs on treatment and follow-up with collaborating centers were recruited into this study from April-December 2020. The proposal was approved by respective ethics committees and written consent was obtained from all the participants.

Data Collection

Clinical information was recorded using a structured case record form (CRF) developed specifically for this study. Subsequently a virtual harmonization meeting was conducted across all the recruiting centers.

Study population

Inclusion criteria for this study participation was a diagnosis of AIRD across all age groups. Their baseline demographics, disease subsets, current and past immunosuppressants (IS) information was recorded, with specific focus on HCQ and GC. We recorded the current dose of glucocorticoid that the patients were using at the time of enrollment. Non-immune mediated rheumatologic disorders and those not on treatment with IS and or HCQ were excluded. All comorbidities, including pre-existing lung diseases were recorded through review of their medical records. Additional medication information including use of antihypertensives such as angiotensin converting enzyme inhibitor (ACEi) or angiotensin receptor blockers (ARBs) were recorded.

Follow-up

The patients were followed up at baseline and then at one, three and six months of initial recruitment. The data was recorded during visits to the hospital or by investigators initiated telephone calls by trained tele-callers (rheumatology nurses/ physician assistants). The information was sought regarding COVID-19 like symptoms, exposure to known COVID-19 patients and results of any COVID-19 test [Reverse Transcription-Polymerase Chain Reaction(RT-PCR) or Rapid Antigen Test(RAT)] using a checklist. Further, those who tested positive on either or both of the tests were considered as suffering from COVID-19 and their outcome information was recorded. The data collection was halted at the time of decline of the first wave of COVID-19 in India. COVID-19 testing protocols for symptomatic infection or exposed contacts were as per Government of India recommendations.

Statistical methods

Descriptive statistics were reported as mean and SD for continuous variables, number and percentages for categorical variables. Association of COVID-19 and mortality with various characteristics of the study population was assessed using Chi square/Fisher’s Exact test and Student t test. Bivariate and multivariate log binomial regression analysis was performed to assess the predictors for COVID-19 positivity and mortality, considering the study design of prospective study (cohort study). Unadjusted and adjusted relative risk along with 95% C.I. were reported. Variables that showed probability value less than 0.10 in the bivariate analysis were considered for multivariate analysis. The unit for person-time for incidence computation (No. of COVID-19 cases/total person time at risk) in this study is reported as person-days which was computed as 177 days (median follow-up) for the study duration. P value less than 0.05 was considered statistically significant. Statistical analyses were carried out using SPSS version 25.0.

Study Population

A total of 9212 AIRD patients were recruited, the major disease subsets were RA(50.9%), SLE(15.4%), axial spondyloarthropathy (SpA)(9.1%) and psoriatic arthritis(PsA)(8%). The mean age of the cohort was 45.1 years (SD 14.3), 2% were in the pediatric age group (< 18yrs) and 77% were females. The median duration of underlying illness was 48 (22,96) months. Their baseline characteristics, IS administered and comorbidities are detailed in Table 1. During the course of this study (median follow up 177 days;(IQR)129,219), 314 patients (3.4%) were diagnosed with COVID-19 based on lab confirmation (RT-PCR and/ or RAT). .

Table 1

Comparison of clinical characteristics amongst COVID and non-COVID AIRD patients
	Overall 9212(%)	COVID positive 314(%)	COVID negative 8898(%)	P value
Age in years	45.1 ± 14.3	46.8 ± 14.3	45.1 ± 14.2	0.028
Gender Male Female	2134 (23.2) 7075 (76.8)	99 (31.5) 215 (68.5)	2035 (22.9) 6860 (77.1)	< 0.001
Duration of AIRD in months 1–24 25–48 > 48	2916 (32.5) 1720 (19.2) 4343 (48.4)	94 (30.4) 64 (20.7) 151 (48.9)	2822 (32.5) 1656 (19.1) 4192 (48.4)	0.658
Diagnosis RA SLE Inflammatory Myositis Systemic Sclerosis Systemic vasculitis PsA Sjogren’s AxSpA Sarcoidosis Bechets Others	4558 (50.9) 1379 (15.4) 99 (1.1) 173 (1.9) 193 (2.2) 716 (8.0) 148 (1.7) 819 (9.1) 58 (0.6) 11 (0.1) 720 (8.0)	120 (40.0) 36 (12.0) 3 (1.0) 7 (2.3) 18 (6.1) 21 (7.1) 8 (2.7) 33 (11.1) 4 (1.3) 2 (0.7) 45 (15.0)	4438 (51.3) 1343 (15.5) 96 (1.1) 166 (1.9) 175 (2.0) 695 (8.1) 140 (1.6) 786 (9.1) 54 (0.6) 9 (0.1) 675 (7.8)	<0.001 0.07 0.25 0.36 <0.001 0.52 0.15 0.31 0.84 0.15
HCQ use N( %) Mean dose(mg) Duration(months)	5266 (57.4) 212 (200,300) 12 (3, 39)	167 (53.2) 200 (200, 300) 10 (0, 38)	5099 (57.5) 200 (200, 300) 12 (3, 39)	0.125 0.136 0.218
Glucocorticoids N(%) Mean dose mg/day (%) < 7.5 7.5–20 > 20	3459 (37.7) 2652 (79.5) 395 (11.8) 289 (8.7)	122 (39.0) 91 (73.4) 22 (17.7) 11 (8.9)	3337 (37.7) 2561 (79.7) 373 (11.6) 278 (8.7)	0.647 0.111
Immunosuppression Methotrexate Azathioprine Mycophenolate Cyclophosphamide Leflunomide Tacrolimus Rituximab TNFi Secukinumab JAKinibs Iguratimod	5494 (60.0) 389 (4.3) 720 (7.9) 58 (0.6) 1811 (19.7) 496 (5.5) 149 (1.6) 193 (2.1) 35 (0.4) 21 (0.2) 42 (0.5)	152 (49.0) 12 (4.0) 34 (11.3) 8 (2.7) 46 (15.1) 15 (5.0) 11 (3.5) 10 (3.2) 1 (0.3) 0 1 (0.3)	5336 (60.3) 377 (4.3) 686 (7.8) 50 (0.6) 1765 (20.1) 481 (5.5) 138 (1.6) 183 (2.1) 34 (0.4) 21 (0.2) 41 (0.5)	0.001 0.800 0.029 <0.001 0.034 0.655 0.007 0.170 0.857 1.000 1.000
Comorbidities DM HTN Pre existing Lung disease	993 (10.9) 1385 (15.0) 366 (4.0)	62 (19.8) 68 (21.9) 28 (8.9)	931 (10.5) 1317 (14.9) 338 (3.8)	<0.0001 < 0.0001 < 0.0001
ACEi /ARBs Yes No	898 (9.9) 8218 (90.1)	39 (12.6) 269 (97.1)	859 (9.8) 7841 (97.7)	0.096
Smoking	85 (0.9)	9 (2.9)	76 (0.9)	0.002
Abbreviations: AIRD-Autoimmune rheumatic diseases, RA- rheumatoid arthritis, SLE- systemic lupus erythematosus, PsA- psoriatic arthritis, AxSpA- Axial spondyloarthritis, HCQ-hydroxychloroquine, TNFi- tumor necrosis factor alpha inhibitor, JAKinibs- janus kinase inhibitors, DM- diabetes mellitus, HTN- hypertension, ACEi- angiotensin converting enzyme inhibitor, ARB- angiotensin receptor blocker

HCQ and COVID-19 Overall, during the study period, 57.4%, 68% and 88% of the total cohort, RA and SLE, were being treated with HCQ respectively. The mean dose of HCQ was 212 mg/day (SD 119). The median duration of HCQ use in the entire cohort was 12 months (IQR 3,39). In the overall cohort, HCQ use did not influence occurrence of COVID-19 (RR = 0.909, CI (0.715,1.154), p = 0.432) or mortality(p = 0.097) (Table 2, Fig. 1). In the subgroup analysis of RA and SLE, there was no independent impact of HCQ on occurrence and outcome of COVID-19.

Table 2

Factors associated with Mortality (N = 13) in COVID-19 population:
	Unadjusted			Adjusted
	RR	95% C.I.	P value	RR	95% C.I.	P value
Age	1.053	1.012, 1.096	0.071	1.037	0.996,1.079	0.079
Gender M:F	1.357	0.456,4.044	0. 583
RA	1.475	0.487, 4.465	0.492
SLE	0.659	0.088,4.955	0.685
Duration of AIRD(months) 1–24 25–48 > 48	1 1.606 0.393	0.534, 4.835 0.048, 3.201	0.399 0.383
Diabetes Mellitus	3.471	1.209, 9.960	0.021
Hypertension	2.233	0.755, 6.607	0.146
Pre-existing Lung involvement	6.362	2.231, 18.130	0.001	4.315	1.416, 13.150	0.010
Current Steroid use	2.23	0.87–5.71	0.09
HCQ	0.341	0.096, 1.215	0.097
CYC	2.41	0.36,16.1	0.362
MMF	1.689	0.511, 5.57	0.390
Rituximab	1.72	0.25,11.8	0.581
ACEi/ARB	1.259	0.290, 5.468	0.759
Smokers	6.162	1.593, 23.83	0.008
Multivariate model adjusted for age, gender, diabetes, Lung involvement
Abbreviations: AIRD-Autoimmune rheumatic diseases, RA- rheumatoid arthritis, SLE- systemic lupus erythematosus, HCQ-hydroxychloroquine, CYC- cyclophosphamide, MMF- mycophenolate mofetil, ACEi- angiotensin converting enzyme inhibitor, ARB- angiotensin receptor blocker

GC and COVID-19 (Table 1, Fig. 1): In the entire cohort, 3459 (37.7%) AIRD patients were using GC at the time of participation in this study. The use of GC was analysed in 3 groups viz. <7.5 mg, 7.5-20mg, and > 20 mg, almost four fifths (79.1%) of them were using a dose < 7.5 mg/day. In the univariate analysis, those on GC moderate dose category (7.5–20 mg), had the highest risk (RR = 1.72, C.I.(1.12,2.64), p = 0.01) of COVID-19, which was substantiated in multivariate analysis (RR 1.57, C.I.(1.003,2.47), p = 0.048). Current use of steroids trended towards moderate influence on mortality as well (RR 2.23, CI (0.87–5.71) p = 0.09)(Table 2).

Associations with other IS, biologics

A higher association of COVID-19 positivity was noted with the use of cyclophosphamide (CYC) (p < 0.001) and mycophenolate (MMF) (p = 0.029) (Table 1). Amongst those on treatment with biologics, risk was higher with rituximab (RTX) (p = 0.007). In the multivariate analysis, CYC [RR-4.2, CI (2.23, 7.91) p < 0.001] and RTX [RR 2.4, CI 1.35–4.32 p = 0.003] use were independent risk factors associated with increased risk of COVID-19. (Fig. 1, supp Table 1). However, MMF (P = 0.39), CYC (P = 0.36) and RTX (p = 0.58) use did not affect mortality (Table 2).

Disease subsets

We found that a lesser proportion of RA patients developed COVID-19 compared to other AIRDs (RR-0.642, CI 0.51, 0.81, p = 0.041). Patients with systemic vasculitis had the highest risk of contracting COVID-19 (p < 0.001) (Table 1).

Comorbidities and other parameters

The parameters associated with COVID-19 positivity were older age (p = 0.025), male sex (p = 0.001), smoking (p = 0.002), comorbidities such as DM (p < 0.0001), HTN (p < 0.0001), presence of underlying lung disease such as interstitial lung diseases, asthma, chronic obstructive pulmonary disease (p < 0.0001) and smoking (p = 0.002). The relative risk of occurrence of COVID-19 with 95% CI is detailed in Fig. 1 (Supp Table 1). Multivariate analysis adjusted for age, gender, DM, HTN, lung involvement and immunosupression use, it was noted that male sex [RR-1.51, p = 0.001], the coexistent DM [RR-1.64, p = 0.001], underlying lung disease [RR-2.01, p < 0.0001], and smoking [RR-3.32, p < 0.0001] were the independent risk factors associated with the increased risk of COVID-19. Use of ACEi/ARBs had no significant impact on occurrence or outcome.

Outcome of COVID-19

Among the 314 COVID-19 patients, 13(4.14%) died. The factors associated with death are presented in Table 2. The pre-existing lung disease was the strongest risk factor associated with increased risk for mortality (RR 4.315, CI (1.416, 13.15)p < 0.01) apart from conventional risk factors such as smoking and coexisting DM.

Comparison of Incidence and mortality of COVID-19 with general population

The incidence of COVID-19 in our cohort was significantly higher compared to the incidence in the general population (p < 0.0001) both in Karnataka and Kerala as depicted in Table 3. Case fatality rate was 4.5 times higher (4.1% in AIRD cohort vs 0.9% in the general population) amongst the AIRD population.

Table 3

Comparison of COVID-19 incidence and mortality in AIRD vs general population:
	Karnataka		Kerala
	AIRD cohort	General population	AIRD cohort	General population
Incidence rate	17.5 /100,000 population	5.3 /100,000 population	25.3/100000 population	7.9/100000 population
Case Fatality rate (%)	9/221 4.1%	12080/918473 1.31%	4/93 4.3%	3070/760692 0.4%
Incidence rates and mortality (p < 0.001), significantly different between Karnataka and Kerala states (both AIRD and general population)

This longitudinal prospective study in AIRD patients was envisaged and initiated before we knew about differential risk factors for SARS-CoV2 infection and the impact of immunomodulatory therapies on its occurrence and outcome. Our AIRD cohort consists of a population from Karnataka and Kerala, who were followed up for approximately 6 months paralleling the time period of 1st peak of COVID-19 in our country. The incidence rate of COVID-19 was 3 fold higher in our cohort; as compared to the general population in the same region.(Table 3) Wang et al in systematic meta-analysis which included data from 26 studies and about 2000 patients, reported 1.5 times higher risk for COVID-19 in rheumatic patients (OR = 1.53, 95% CI 1.24–1.88)(8). In our cohort involving more than 9000 patients, RR is more than 3 times that of the general population.

Many of AIRD such as RA and SLE are treated routinely with HCQ, whereas others such as SpA, PsA and primary vasculitis are not. This brings out a natural selection of a cohort with and without the HCQ in AIRD, and allows us to assess the differential effect of this drug on occurrence as well as outcome of COVID-19. After adjusting for confounding variables, in our AIRD cohort, long term HCQ use did not influence either occurrence or mortality of COVID-19. The role of HCQ in COVID-19 prevention and management has been greatly debated with several authors reporting conflicting outcomes.(9–11) The potential beneficial role of HCQ in terms of preventing viral entry and replication may be fractional and its immunomodulatory effect could possibly be offset by the interaction with antiviral drugs in the acutely sick patients.(12) Through our study, we could not substantiate its role as a prophylactic immunomodulator despite its use for many months or years. Hence, we emphasize the use of HCQ in AIRD based on its need for the underlying rheumatic disease and not with respect to COVID-19 pandemic.

In our cohort, we found an increased rate of infections with GC in moderate doses of 7.5–20 mg of prednisolone equivalent per day(RR 1.57) and not in lower dose (< 7.5mg/day). Glucocorticoids have been consistently reported to increase the risk of both opportunistic and serious infections in AIRD.(13) However, in COVID-19 related hyperinflammation they have therapeutic value. Favalli et al. demonstrated a higher risk of COVID-19 in patients with AIRD, even at doses less than 2.5 mg/day of prednisolone (OR of 2.89).(14) In its initial report of over 600 cases of COVID-19 in AIRD, Global rheumatology analysis network found a two times of odds of hospitalization with the use of steroids of > 10 mg prednisolone /day.(15) Marques et al. demonstrated GC use to be associated with unfavorable outcomes of COVID-19 in rheumatic diseases.(16) With the demonstration of significant mortality benefit by the use of Dexamethasone in RECOVERY trial there has been renewed interest in the use of steroids in COVID-19.(6) In our study, we could not determine decisively the impact of steroids on mortality though there was a trend towards it. In line with the American College of Rheumatology recommendations, we advocate minimization of steroid use in AIRD to the lowest possible dose and shortest duration wherever feasible till further conclusive date becomes available.(17)

Amongst other ISs, MMF and CYC confer substantially higher risk of COVID-19 occurrence and mortality in our cohort, while other IS/csDMARDS did not have any major impact. Scire and colleagues from Italy; also found no significant influence of csDMARDs on the risk of hospitalization or mortality {OR 0.54[CI 0.22–1.37] p = 0.188}.(18) Overall, the effect of csDMARDs on COVID-19 risk appears to be minimal. At the very least, appropriate use of csDMARDs for the underlying AIRD must not be withheld in the wake of COVID-19 pandemic.

Among the biologicals and targeted synthetic DMARDs (tsDMARDS), RTX exposure was greater among the COVID-19 patients in our cohort (3.5% vs 1.6%) (Table1). In a French cohort, RTX use was associated with the higher risk of severe COVID-19 disease (RR 3·26).(19) Pablos et al. from Spain reported a higher incidence of COVID-19 in AIRD treated with bDMRADS/ tsDMARDS {OR 1.60 95% CI (1.23–2.10)} but not in those with cDMARDS.(20) Interestingly, at least 3 reports from different parts of the world describe a reduction in risk of severe COVID-19 with the use of bDMARDs particularly TNFi.(18, 21, 22) There was no significant influence of TNFi or secukinumab use in our cohort. Because of small numbers and the differential dosing regimens of bDAMRDs, we can not derive any conclusion on their exact role in the outcome or occurrence of COVID-19. Similarly, the tsDMARDs did not influence the occurrence or outcome of COVID-19 in our cohort.

RA was the most common disease subset in our cohort, being the most common AIRD in the community. Patients with systemic vasculitis had the highest risk of contracting COVID-19 (18/189; 9.5%), while RA had a lower risk (2.6%). This could be related to the use of higher doses of steroids and more intense IS in systemic vasculitis compared to other disease subsets.

Most important risk factors for developing COVID-19 in our cohort were older age, male sex smoking, underlying comorbidities such as DM, HTN and pre-existent lung disease. Early on in this pandemic, it was understood that DM and HTN form the major risk for COVID-19.(23, 24) Bhandari et al. reported HTN and DM to be the major underlying conditions from Jaipur, India in 522 COVID-19 patients.(25) Pre-existing lung disease and smoking carried the highest risk of COVID-19 related mortality in our cohort. This is similar to other cohorts of COVID-19 in rheumatic diseases where DM, HTN, age > 65 years and pre-existent lung disease were responsible for poor outcome.(26) A recent Brazilian study of a cohort of AIRD also found a higher requirement for emergency care in diabetic patients compared to non-diabetics (OR 1.38; p = 0.004).(16) A recent meta-analysis also confirmed HTN (OR = 3.69, 95% CI 1.41–9.69, P = 0.008) and lung disease (OR = 2.93, 95% CI 1.64–5.23, P = 0.000) to predict hospitalization risk.(8) Therefore, the risk factors which increase the susceptibility to COVID-19 and adverse outcome in the general population hold true even in patients with AIRD.

Gender bias among patients with COVID-19 is a globally documented phenomenon. It is postulated to be the effect of sex hormones, stronger interferon response, higher helper & cytotoxic activity of T cells, differential ACE2 expression and several others.(27, 28) In our COVID-19 infected cohort too, there was a preponderance of males in the multivariate analysis (RR = 1.51), however it did not influence mortality. Even though the number of smokers was relatively small in our cohort (< 1%), association with the COVID-19 disease was quite noteworthy (p = 0.002). Leung et al demonstrated increased ACE2 gene expression in the bronchoalveolar lavage samples of smokers versus never-smokers to be the reason for increased susceptibility.(29) However, the data from China and Italy indicated a lower risk of COVID-19 and severity in current smokers.(30, 31) On the contrary, a large UK study involving more than 2.4 M participants has demonstrated a higher risk of COVID-19 in current smokers (OR 1.14).(32)

Initial concerns regarding the use of ACEi/ ARBs surfaced due to the upregulation of ACE 2 receptors on epithelial cells which are a portal of entry for SARS-COV-2.(33, 34) However, a meta-analysis of 10 studies, found neither the risk of COVID-19 nor severity of infection to be increased with the use of ACEi/ ARBs.(35) In our cohort of patients with AIRD, we found no significant impact of ACEi/ ARB use on the risk of COVID-19 occurrence or mortality.

Case fatality rate in our cohort was 4.14% which is 4.6 times higher than that in the general population from the same geographic area (0.9%). OpenSAFELY- an initiative by the NHS, analysed the risk factors associated with the occurrence of COVID-19 in more than 1.7 Million people in England.(36) They found a higher risk of COVID-19 related death (OR of 1.3) in patients with RA, SLE and Psoriasis.(36) In another systematic review and meta-analysis, the fatality rate was 7% in the entire analysis and 6.7% in the GRA cohort. (15)(37) In our cohort, the strongest risk factor associated with mortality was underlying lung disease. Furthermore, the disease subsets, immunosuppressants and other comorbidities did not have much influence on mortality in our cohort.

To the best of our knowledge this is the first longitudinal cohort of impact of COVID-19 on any immunocompromised or immunodeficient cohorts from India. Initial results of this cohort were published during the early part of the pandemic.(38) Strengths of our study are its prospective longitudinal non-interventional design, large sample size from specialist rheumatology centres, inclusion of RT-PCR or RAT confirmed COVID-19 patients and investigator initiated follow-up telecalls. Limitations of our study include inability to accurately assess the impact of disease activity on occurrence and outcome of COVID-19. As rheumatologists are not the primary physicians for COVID-19 care, we were not able to access precise information with regards to O2 therapy, hospitalization and ICU care. Also, the testing for COVID-19 in individual patients was as per Govt. of India, Govt. of Karnataka and Govt. of Kerala protocols which have undergone modifications as the pandemic progressed. Our data may not have captured all asymptomatically infected patients in this analysis. This could have biased the result and might have altered the reported incidence as well as mortality. Also the comparison of incidence and mortality of COVID-19 in AIRD with the general population might have been influenced by the differential age and sex composition in both the populations.

Long term HCQ use had no significant impact on COVID-19 occurrence and mortality in AIRD patients while moderate doses of GC increased the risk of infection. AIRD patients who are elderly, male, smokers, hypertensive, diabetic and those with underlying lung disease have a higher risk of contracting COVID-19. The incidence rate is at least 3 fold higher and the case fatality rate is 4.6 times higher than that of the general population in the same geographic region during the same time period. Hence, this group of AIRD regardless of age and other comorbidities, merits first access to the various protective measures implemented against COVID-19 .

Ethics approval and consent to participate: Ethics approval was obtained from respective ethics committee (St. John’s Medical College-IERB No-127/2020, Chanre Rheumatology and Immunology Center & Research - CRICR/SN-130/099/2020, JSS Medical College-JSSMC/IEC/180820/12NCT/2020-21). Written consent was obtained from all the participants.

We hereby confirm that approval was provided for the study titled ‘Role of HCQ in COVID-19 pandemic in Rheumatological disorder”

Consent for publication: consent to publish from the patient- Not Applicable

Availability of data and materials: The data and materials are available to all authors

Competing interest- None of the authors has any conflict of interest to disclose.

Funding: Karnataka Rheumatology Association

Authors Contribution:

1) Conception and design of the study- V, CS

2) Analysis and interpretation of data- VS, AP, CK, CS

3) Data acquisition - All authors

4) Drafted the work and revised it critically for important intellectual content – AP, CK, VS, CS, VKR, BP

5) Approved the version to be published- All authors

6) Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved- All authors

Ethical Publication: We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines.

Acknowledgement: We thank Ms Sumithra Selvam, Department of Biostatistics, St John’s Research Institute, SJNAHS, Bengaluru, for her support.

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No competing interests reported.

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A Prospective Longitudinal Study Evaluating The Influence of Immunosuppressives and Other Factors On COVID-19 in Autoimmune Rheumatic Diseases

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Abstract

Background

Methods

Results

Conclusions

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Key Message

Introduction

Methods

Results

Discussion

Conclusions

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References

Additional Declarations

Supplementary Files

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