The mixture of different bioactive constituents from plant extracts and several other natural products produces synergistic effects. This synergistic activity aids in treating and preventing various diseases by reducing the usage of conventional drugs. The present investigation aimed to study the effect of poly-herbal formulations of selected plants (Turmeric, Pepper, Neem, Vinca rosea ) on improved biological activity with and without pulsed electric fields(PEF). Selected plant extracts were combined in different proportions(1% to 3%) to formulate a poly-herbal mixture. Ethanolic crude extracts were extracted by Soxhlet method and their phenolic activity and antioxidant activity were quantified. Further, to enhance the recovery of intracellular components, electrical fields of different parameters were used. The effect of PEF on formulated mixtures was compared with untreated extracts, which exhibited higher phenolic and antioxidant activity than untreated extracts. Out of all combinations, turmeric with pepper showed higher phenolic and antioxidant activity, and hence, these combinations were checked for their potential cytotoxicity against MCF-7 Estrogen breast cancer cells. In addition, different electrical ((EP1)High intensity,short duration & (EP2)Low intensity,long duration) pulses were used to enhance the uptake of extracts into the cell membrane. Lowest cell viabilities of 19.41%(raw extract),14.75%(pulsed extract),13.28%(pulsed extract+EP1) and 9.982%(Pulsed extract+EP2) after 72hrs of treatment for 1000μg/ml concentration of turmeric(97%) and pepper(3%) mixture was observed. This shows the potential of the synergistic effect of electrical pulses and poly-herbal mixture, achieving phenolic and antioxidant-rich extracts causing higher cell death. This technique can be used as an effective method to treat breast cancer avoiding undesirable side effects due to higher doses of synthetic drugs and single herb.