Background
In the present study, PPAR-γ antagonist was used to induce preeclampsia model in mice to explore the intervention effect and mechanism of aspirin on preeclampsia model mice.
Methods
Pregnant mice were injected intraperitoneally with PPAR-γ antagonist (2 mg/kg/d) at 8.5-12.5 days of pregnancy to establish PE mouse models. Two doses of LDA (10 mg/kg/d and 20 mg/kg/d) were given to the PE mouse models for intervention.The blood pressure, 24-hour proteinuria, urine protein/creatinine ratio, interleukin (IL)1-β and endoglin level in peripheral blood, placental PPAR-γ mRNA expression in placenta, the placenta weight and pathology of placenta and kidneys of mice from different groups were detected.
Results
LDA effectively decreased the blood pressure increase caused by PPAR-γ antagonist in mice, and the increase degree of urinary protein and urinary protein creatinine ratio was notably relieved.LDA also inhibited the overexpression of Endoglin and IL-β induced by PPAR-γ antagonist.In addition, LDA evidently increased the placental weight of preeclampsia mice.The placenta and kidney injury were obviously less serious.LDA alleviated the expression inhibition of PPAR-γ mRNA.The remission effect of 20mgLDA was significantly better than that of 10mg.
Conclusions
(1) LDA has a preventive effect in PE mice induced by PPAR-γ antagonist. (2) The preventive effect of LDA is dose-dependent in PE mouse models induced by PPAR-γ antagonist.