Ticks act as vectors for many zoonotic pathogens and are an increasing threat to human and animal health, but tick-borne pathogens (TBPs) are not the only important residents inside ticks; they coexist with many other microorganisms. Tick microbial communities can affect tick health and immunity, and they can also interact directly with tick-borne pathogens. A recent study sought to characterize tick-borne microbes and their temporal dynamics and interactions. Using high-throughput sequencing, researchers studied the dynamics of microbes in specimens of the tick species Ixodes ricinus collected over 3 years in a peri-urban forest. They found that around 75% of sequences belonged to maternally inherited bacterial genera. The structure of the tick microbiota varied over time, with the greatest changes driven by environmental microbes. Strong relationships were observed between Wolbachia and Arsenophonus, providing evidence for the presence of the parasitoid wasp Ixodiphagus hookeri in ticks, and network analysis suggested that the presence of pathogens belonging to the genera Borrelia, Anaplasma, and Rickettsia may disrupt microbial interactions in I. ricinus. These data provide valuable insight into the temporal dynamics and interactions of tick-borne microbes, paving the way for the development of new strategies to control ticks and tick-borne diseases.