Enhancer Promoter Interactome and Mendelian Randomization Identify Network of Druggable Vascular Genes in Coronary Artery Disease

doi:10.21203/rs.3.rs-978033/v1

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Enhancer Promoter Interactome and Mendelian Randomization Identify Network of Druggable Vascular Genes in Coronary Artery Disease

https://doi.org/10.21203/rs.3.rs-978033/v1

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published 04 Mar, 2022

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Coronary artery disease (CAD) is a multifactorial disorder, which is partly heritable. Herein, we implemented a mapping of CAD-associated candidate genes by using genome-wide enhancer-promoter conformation (H3K27ac-HiChIP) and expression quantitative trait loci (eQTL). Enhancer-promoter anchor loops from human coronary artery smooth muscle cells (HCASMC) explained 22% of the heritability for CAD. 3D enhancer-promoter genome mapping of CAD-genes in HCASMC was enriched in vascular eQTL genes. By using colocalization and Mendelian randomization analyses, we identified 58 causal candidate vascular genes including some druggable targets (MAP3K11, CAMK1D, PDGFD, IPO9 and CETP). A network analysis of causal candidate genes was enriched in TGF beta and MAPK pathways. The pharmacologic inhibition of causal candidate gene MAP3K11 in vascular SMC reduced the expression of athero-relevant genes and lowered cell migration, a cardinal process in CAD. Genes connected to enhancers are enriched in vascular eQTL and druggable genes causally associated with CAD.

Epigenetics & Genomics

Atherosclerosis

Coronary artery disease

Genes

3D genome

Mendelian Randomization

Druggable genome

Network

Coronary artery disease (CAD) is a complex trait disorder and a leading cause of morbidity-mortality. The dysregulated expression of genes and activation of pathways culminate in the development of vascular atheromatous plaque, a hallmark feature of CAD¹. Functional assessment and cell tracking experiments have shown that vascular smooth muscle cells are recruited to the plaque where they play a significant role in the development of CAD². The identification of molecules involved in the pathophysiology of CAD could lead to the development of novel therapies. However, the discovery of disease-associated drug targets is limited by several factors, which include limited information about the disease process and indirect evidence obtained from animal and cell experiments³. Also, epidemiological studies carried out in humans by measuring biomarkers or intermediate molecules is subject to bias and reverse causality⁴. As such, only a small fraction of drug development programs leads to licensed drugs⁵.

Genetic association studies (GWAS) have underlined that CAD is heritable (narrow sense heritability estimated at 40-50%) and involves several loci (~160 identified so far)⁶. Genetic association data provides a rich resource, which may help identify key targets involved in the development of disorders. Gene variants associated with intermediate phenotypes (e.g. gene expression) and the disease can be leveraged to assess causality similarly to a randomized clinical trial⁷. According to Mendel’s second law of random allocation of alleles, independent instrumental variables (IVs) (uncorrelated variants) can be assessed to mimic the effect of drugs (exposure, e.g. as determined by gene expression) on the risk of disorders (outcome). The use of multiple genetic variants that are associated with the exposure can be used as IVs in Mendelian randomization (MR) to assess causal associations for the risk of disorders⁸. Since the allocation of alleles is random and occurs before the development of the outcome, MR technique is not prone to bias and reverse causation⁹. Studies have underlined that pharmacological targets supported by genetics have a much higher chance of success during the different phases of drug development¹⁰.

Despite rigorous preclinical screening, the failure of some compounds is related to drug-related adverse side-effects, which are often discovered in large phase 3 randomized clinical trials (RCTs)¹¹. This may result in substantial monetary loss with the attrition of resources for the development of novel drug pipelines¹². Target-related pleiotropy is one cause of such failure¹⁰. For instance, a drug target with opposite directional effect on two major outcomes may negate potential benefit. Interrogation of large electronic health record databases in genotyped individuals provides a resource to assess hundreds of traits and disorders. The assessment of risk variants linked to a target in a phenome-wide analysis study (PheWAS) is thus a strategy to evaluate potential side-effects of drug-gene pairs¹³. Also, the assessment of putative causal candidate drug targets for their association with monogenic disorders by using large resources where data are collated, such as in the Human Phenotype Ontology, is another approach to assess drug-related safety issues^14,15.

Complex systems in which gene expression and interaction of molecules are establishing a trajectory to health or disease are increasingly investigated by the integration of data in network¹⁶. The network topology is highly modular and provides information for distinctive molecules, which interact together to drive different functions¹⁷. As such, genes highly connected tend to be enriched in essential functions and in drug targets^18,19. The implementation of network to assess molecules with impact on the biological pathways relevant to a disease is a useful strategy to prioritize genes and to address the function of the whole system and its components (modules).

In order to identify causal genes by using genome-wide association studies (GWAS), we need to map variants to potential gene targets. Mapping of genes is compounded by several factors, which includes the linkage disequilibrium (LD) between the variants (the extent to which variants are correlated) and the fact that the vast majority of gene variants associated with complex traits and disorders reside in the noncoding genome²⁰. Variants within the noncoding genome are enriched in active regulatory regions such as distant acting enhancers and may impact on the expression in cis (locally) through the conformation of chromatin²¹. Genome-wide assessment of chromatin conformation based on Hi-C and its derivatives (e.g. HiChIP) has revealed that chromatin looping between enhancers and promoters provides regulatory mechanisms to control gene expression²². Growing evidence suggests that a hierarchy among the distant regulatory regions provides another layer of control on gene expression²³. To this effect, highly connected hub enhancers in 3D communities regulate lineage-specific genes²⁴. In addition, the architecture of chromatin conformation is largely cell specific²⁵. Hence, GWAS mapping using genome-wide 3D data provides an additional layer of information to identify putative causal genes in disease-relevant cells²⁶. Another strategy to map the genetic variants to potential targets is to assess the associations with expression quantitative trait loci (eQTL) derived from disease-relevant tissue(s)²⁷. Herein, we implemented an integrative approach including enhancer-promoter chromatin conformation and eQTL mapping, causal inference, interrogation of the druggable genome and network biology to capture GWAS-associated molecules and pathways, which could be targeted pharmacologically. Specifically, we assessed whether enhancer-promoter chromatin looping in vascular smooth muscle cells explained a significant proportion of the heritability for CAD and if it was enriched in vascular eQTL genes. Genome-wide mapping using enhancer-promoter conformation and eQTL data were integrated in a framework to assess causal associations and to prioritize druggable genes with the help of networks.

Tissue enrichment and pathway analyses

Summary-level data of meta-analysis from UK Biobank (UKB) and CARDIoGRAMplusC4D including 123,733 CAD cases and 424,528 controls²⁸ was leveraged to assess tissue and pathway enrichment. We implemented Data-driven Expression Prioritized Integration for Complex Traits (DEPICT) to document the enrichment of tissue and pathways for genetic association data in CAD. By using GWAS summary statistics, DEPICT provides an analysis in which risk loci mapped genes are integrated to a vast collection of human tissue gene expression to prioritize highly expressed genes and their relevant pathways. According to DEPICT, genetic association for CAD was enriched in arteries (P=7.38 x 10⁻⁹) and smooth muscle (P=4.33 x 10⁻⁶) (Figure 1A) (Suppl. Table 1). We next evaluated the enrichment of genetic association in pathways. DEPICT showed an enrichment for abnormal cell adhesion (P=5.04 x 10⁻¹⁴), abnormal vitelline vasculature morphology (P=5.64 x 10⁻¹³) and abnormal cell migration (P=3.67 x10⁻¹¹) (Figure 1B) (Suppl. Table 2). These data thus indicated that the vasculature and smooth muscle cells as well as their related functions such as adhesion and migration are key features associated with CAD-gene variants.

Heritability for CAD explained by the vascular enhancer-promoter connectome

Considering the high enrichment of genetic association data for CAD in smooth muscle and arteries, we analyzed publicly available HiChIP for H3K27ac obtained in human coronary artery smooth muscle cells (HCASMC) (GSE101498). H3K27ac-HiChIP provides a high-definition map of chromatin conformation between enhancers and promoters²⁹. After a stringent loop call with FitHiChIP (FDR < 1 x 10⁻⁶), we identified 224,209 confident loops in HCASMC. The anchor loops were significantly enriched in open chromatin detected by assay for transposase-accessible chromatin and sequencing (ATAC-seq) in HCASMC (fold enrichment=2.9, P<2.2 x 10^−16, binomial test) (Figure 1C). By using HOMER, a pathway enrichment of anchor loops in HCASMC using the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed an enrichment for endocytosis (P=1.36 x 10⁻¹⁰), regulation of actin cytoskeleton (P=1.70 x 10⁻⁸) and autophagy (P=5.54 x 10⁻⁸) (Suppl. Table 3). We next wondered whether enhancer-promoter contacts in HCASMC explained a significant part of the heritability in CAD risk. For that purpose, we partitioned the heritability of cis-anchor loops in HCASMC by using the full baseline model of 53 annotations in the stratified LD score regression framework (Methods)³⁰. This analysis revealed that chromosomal interactions in HCASMC were enriched for regions that explained the heritability of CAD (enrichment=2.38, P=5.90 x 10⁻⁵) (Suppl. Table 4). Despite representing only 9% of CAD-single nucleotide polymorphisms (SNPs), variants within anchor loops in HCASMC explained 22% of the heritability for CAD (Suppl. Table 4).

Identification of gene promoters connected to CAD associated variants within distant enhancers

To identify gene promoters mapped by chromatin looping in H3K27ac-HiChIP in HCASMC, we identified lead and individual significant SNPs (P_GWAS<5 x 10⁻⁸, r²<0.6) that overlapped with enhancer loops (Methods). In total, 353 individual significant SNP-enhancer-promoter loop pairs tagging 228 gene promoters were identified (Suppl. Table 5). The mean number of loops per gene promoter was 1.5 and the average distance between enhancers and gene promoters was ~ 168kb. For instance, at 11q13.1, rs3741380, a CAD individual significant SNP intronic to EHBP1L1, is localized within an interacting loop with the promoter of MAP3K11 located ~32kb downstream (Figure 1D). We next hypothesized that CAD gene variants may be enriched in hub enhancers having a high level of chromatin contacts (in network with a degree ≥ 90th percentile) (Methods). In HCASMC, we identified 5,455 highly connected hub enhancers involved in chromatin looping. This analysis showed a significant enrichment of CAD individual significant SNPs associated with hub enhancers (observed/expected=3.4, P=1.9 x10⁻⁶, binomial test). Among the different genes mapped by CAD variants and enhancer-promoter looping, SH2B3 is a gene connected to a hub enhancer. SH2B3 has been previously identified as a causal candidate gene for CAD³¹. On the other hand, some genes identified by chromatin looping were not previously mapped in CAD. For instance, at 11q13.4, rs590121 is located in a distant hub enhancer intronic to SERPINH1 and having contacts with multiple enhancers-promoters including with the promoter of ARRB1 located ~298kb upstream (Figure 1E).

Identification of vascular eQTLs

We next examined if CAD-associated genes mapped by enhancer-promoter chromatin looping were also significant vascular expression quantitative trait loci (eQTL). CAD-individual significant SNPs were mapped to eQTLs of the aorta in GTEx v8. In total, 15,516 CAD SNP-eQTL gene pairs were significant at FDR 5% in the aorta and tagged 202 genes (Suppl. Table 6). Among the 228 genes mapped by enhancer-promoter chromatin looping there were 41 genes (18%), which were also CAD-associated eQTL genes (fold enrichment observed/expected=21.4, P < 2.2x10⁻¹⁶, binomial test). Hence, these findings highlight that gene mapping of CAD genetic association data with enhancer-promoter chromatin conformation in HCASMC is enriched in vascular eQTL genes.

Genetic colocalization analyses

By combining enhancer-promoter chromatin looping and eQTL data, there were 383 genes mapped by CAD-associated SNPs. We performed Bayesian colocalization analyses between the eQTL signal (GTEx v8) of the 383 mapped genes in the aorta with the GWAS signal for CAD. We identified 35 genes with a high posterior probability (PP) (PP > 0.8) of shared signal between eQTLs in the aorta and CAD-GWAS (CDH13, PHACTR1, TCF21, N4BP2L2, SYPL2, TWIST1, PDGFD, IPO9, FHL3, UTP11, GGCX, SEMA5A, DMPK, MIA3, TMEM133, CAMK1D, ARHGAP42, DAGLB, DMWD, CETP, MORF4L1, JCAD, MFGE8, HAPLN3, HHIPL1, DHX36, B3GNT8, BMP1, LMOD1, FAM117B, MAT2A, ATP2B1, EXOSC5, EIF2B2, ZEB2) (Suppl. Table 7). Genes with a shared signal were enriched in gene ontology (GO) for negative regulation of cell adhesion (P=1.99 x 10⁻⁴) and positive regulation of endothelial cell proliferation (P=2.18 x 10⁻⁴) (Suppl. Table 8). Figure 2A shows a LocusCompare plot for the GWAS associations at 6p24.1 where there is a shared signal (PP=1) with the expression of PHACTR1, a gene previously identified as a causal candidate at this locus³². Rs9349379 is the strongest SNP for both GWAS and eQTL in the aorta for the expression of PHACTR1, a gene encoding for a regulator of actin polymerization³³. Allele G-rs9349379 increases the risk of CAD (OR: 1.11, 95%CI: 1.10-1.12, P_GWAS=2.71 X 10⁻⁷⁶) and is associated with a decreased expression of PHACTR1 in the aorta (Figure 2B). This analysis underlined some targets identified in previous screen and functional assays such as CDH13, TCF21, LMOD1 and JCAD^34–37, but also identified novel potential causal candidates such as EXOSC5 and B3GNT8, which encode for a RNA exosome component and a galactosyltransferase respectively.

Mendelian Randomization

In order to further evaluate causal associations, we implemented a 2-sample Mendelian randomization (MR) between gene expression in the aorta (GTExV8) by using a minimum of three independent (r²<0.1) cis-variants as IVs and genetic associations for CAD as the outcome. For that purpose 3D and eQTL mapped genes were evaluated in MR. In inverse variance weighted (IVW) MR, we identified 74 vascular genes expressed in the aorta that were significantly associated with CAD (FDR < 0.05). Among these genes, 33 did not show heterogeneity (Cochran’s Q test > 0.05) and were considered as causally associated with CAD (MRAS, HHIPL1, CDH13, JCAD, MFGE8, BMP1, FGD6, CTSK, MAP3K11, TMEM133, CAMK1D, DMPK, ZEB2, EIF2B2, HSD17B12, CDC25A, ARRB1, SFMBT1, TRIP4, KCNH2, NME7, ATP1B1, MRPL35, CCDC181, AGPAT4, RNF123, ANKDD1A, BEND6, CTSH, NPHP3, PIF1, ALKBH5, MEAF6) (Figure 3) (Suppl. Table 9). These 33 vascular genes were enriched for GO in cardiac muscle cell repolarization (P=1.44 x10⁻⁴) and proteolysis involved in cellular catabolic process (P=4.80 x 10⁻⁴) (Suppl. Table 10). Among the causally associated vascular genes, there were 10 genes also identified in colocalization analyses (PP > 0.8) (HHIPL1, CDH13, JCAD, MFGE8, BMP1, TMEM133, CAMK1D, DMPK, ZEB2, EIF2B2). In total, by using colocalization and MR approaches, we identified 58 potential causal vascular genes for CAD (Suppl. Table 11). Among the causal candidate genes, 9 were identified by both eQTL and enhancer-promoter conformation mapping (FHL3, MIA3, MAT2A, GGCX, DAGLB, MAP3K11, ATP2B1, EXOSC5, B3GNT8), whereas 6 genes (MEAF6, AGPAT4, KCNH2, ARRB1, PIF1, UTP11) were mapped only by chromatin conformation. For instance, AGPAT4, a gene encoding for a lysophosphatidic acid acyltransferase, is negatively associated with the CAD risk in MR (OR: 0.94, 95%CI: 0.91-0.97, P_causal=1.5 x 10⁻³). At this locus, two CAD independent significant SNPs (rs9295142, rs142697177) located in an intronic enhancer of AGPAT4 are within a chromatin loop with the promoter of the same gene (Suppl. Figure 1).

In sensitivity analysis, we assessed causal associations by using weighted median MR, which provides a robust assessment with up to 50% of invalid instrumental variables (variants with horizontal pleiotropy)³⁸. Among the 33 vascular genes associated with CAD in IVW MR, we found that 29 genes (MFGE8, CAMK1D, CTSK, JCAD, CDH13, EIF2B2, MAP3K11, FGD6, RNF123, ATP1B1, SFMBT1, MRPL35, MRAS, KCNH2, NPHP3, ANKDD1A, BEND6, CTSH, NME7, CCDC181, HSD17B12, TRIP4, CDC25A, TMEM133, DMPK, ZEB2, ARRB1, BMP1, HHIPL1) were also significant in weighted median MR analysis (Suppl. Table 12). The directional effects were concordant between IVW and weighted median MR analyses.

Network and prioritization

We aimed to characterize putative causal vascular genes singled out by the colocalization and MR screens by using a network approach with the objectives to identify: 1) key driver genes and 2) pathways of connected genes with functional relevance. A network was constructed from the DifferentialNet dataset, which includes 134,223 protein interactions, and inferred to the aorta from the gene expression profile (Methods)³⁹. Candidate causally associated vascular genes identified by MR and colocalization analyses were used as seeds to generate the network, which encompassed 681 nodes (genes) and 763 edges (connections) (Figure 4A). A pathway enrichment analysis showed that genes within the network were enriched in TGF-beta signaling pathway (P=1.87 x 10⁻²⁰), signaling events mediated by HDAC class I (P=3.46 x 10⁻¹⁷), and MAPK signaling pathway (P=1.62 x 10⁻¹⁶) (Suppl. Table 13). Causally associated genes were enriched in nodes with a high degree (degree >90th percentile) (fold enrichment 3.83, P=2.70 x 10⁻²⁵, hypergeometric test). Genes with a high degree and acting as hub in networks are enriched in drug targets and are involved in key biological functions. Predicted CAD causally associated vascular genes in MR and acting as hub gene (high degree in PPI network) include among others ARRB1 (OR: 0.84, 95%CI: 0.78-0.91, P_causal =9.46 x 10⁻⁶), CDC25A (OR: 0.96, 95%CI: 0.95-0.98, P_causal =5.33 x 10⁻⁶), KCNH2 (OR: 1.03, 95%CI: 1.01-1.04, P_causal =7.14 x 10⁻⁵) and MAP3K11 (OR: 1.07, 95%CI: 1.05-1.10, P_causal =8.46 x 10⁻⁹).

Druggability of candidate causal genes

We next assessed whether causally associated vascular genes in CAD were potentially druggable by using The Drug Gene Interaction Database (DGIdb)⁴⁰. DGIdb includes an exhaustive list of drug-gene pairs, which are collated from different resources. In total 383 compounds targeting 13 predicted causally associated vascular genes were identified by DGIdb (CTSK, MAP3K11, CDC25A, ARRB1, KCNH2, ATP1B1, PDGFD, IPO9, GGCX, DAGLB, CETP, DHX36, CAMK1D) (Suppl. Table 14). Considering the directional effects in MR, 5 vascular genes are potential targets for drug inhibition (CTSK, MAP3K11, KCNH2, ATP1B1, CAMK1D). For instance, the vascular expression of CAMK1D which encodes for a calcium calmodulin dependent protein kinase, is positively associated with the risk of CAD in MR (OR: 1.11, 95%CI: 1.07-1.16, P_causal=5.12 x 10⁻⁸) (Figure 4B). Several drugs under investigation are kinase inhibitors, which are reported to target CAMK1D. By using colocalization analyses, the directional effects of the candidate causal SNP between vascular gene expression and CAD-risk suggest that the inhibition of 4 genes may lower disease-associated risk (PDGFD, IPO9, GGCX, CETP). As an example, among the potential drug targets, PDGFD encodes for platelet derived growth factor D. In the aorta, colocalization between eQTL for PDGFD and CAD-GWAS (PP=0.99) suggests a causal association and prioritizes gene variant rs974819 (Figure 4C). In the aorta, T-rs974819 is associated with a higher expression of PDGFD (Figure 4D) and an elevated CAD-risk (OR: 1.06, 95%CI: 1.05-1.07, P_GWAS=1.11 x 10⁻²⁸). According to the directional effects in MR and colocalization data, 4 genes (CDC25A, ARRB1, DAGLB, DHX36) could be targeted by agonist-based therapy. However, for these targets there is no agonist compound reported in DGIdb.

As some potential disease-associated targets may be linked with adverse side-effects, we undertook a phenome-wide analysis (PheWAS) by using Gene ATLAS, which includes 778 diseases-traits from the UK Biobank⁴¹. Genes identified as candidates for drug inhibitors were included in this analysis (CTSK, MAP3K11, KCNH2, ATP1B1, CAMK1D, PDGFD, IPO9, GGCX, CETP). The strongest instrumental variable (lowest P-value) in MR or the variant prioritized in colocalization analyses for each of the potential drug target gene was assessed in the cross-phenotype association analysis. Diseases-traits were deemed significantly associated with the variant at FDR 5% and data are presented in Suppl. Table 15. In GTEx, gene variant A-rs12042263 (strongest IV in MR for the expression) is associated with a lower vascular expression of CTSK (P_eQTL=1.69 x 10⁻¹⁰) and a decreased risk for CAD (OR: 0.96, 95%CI: 0.95-0.98, P_GWAS=1.18 x 10⁻³). In PheWAS, A-rs12042263 is positively associated with the risk of cerebrovascular disease (P_PheWAS=3.15 x 10⁻⁴) (I67 other cerebrovascular disease) (Suppl. Table 15). These data suggest that the inhibition of CTSK may lower the risk of CAD (data in MR are concordant as the vascular expression of CTSK is positively associated with CAD-risk), but at the expense of increasing cerebrovascular events. Several drugs under investigation are reported in DGIdb to inhibit CTSK (Suppl. Table 14). A recent randomized clinical trial evaluating odanacatib, a CTSK inhibitor developed for postmenopausal osteoporosis, has found that inhibition of the target lowered primary endpoints, but increased the risk of stroke (HR 1.32, p=0.03)⁴². Also, inhibition of ATP1B1, a gene positively associated with the CAD-risk in MR, is predicted to increase the risk of venous thromboembolic disease (Suppl. Table 15). Prediction based on colocalization analysis suggests that inhibition of GGCX may lower CAD-risk, but according to the PheWAS it is associated with an increase risk of malignant neoplasm of prostate (P_PheWAS=3.06 x 10⁻⁹) (Suppl. Table 15). We next interrogated the Human Phenotype Ontology database⁴³ to identify disease-trait associations for the genes deemed druggable (Suppl. Table 16). In Human Phenotype Ontology, CTSK, GGCX and KCNH2, have been linked to bone-related conditions, coagulation defects and ventricular arrhythmia respectively. KCNH2 encodes for the Ether-A-Go-Go-Related Protein 1, a potassium voltage-gated channel, involved in arrhythmia. Consistently, in the Side Effect Resource (SIDER)⁴⁴, several drugs targeting KCNH2 in DGIdb are reported to induce ventricular arrhythmia. After a comprehensive filtering based on multiple resources including a PheWAS analysis and interrogation of Human Phenotype Ontology as well as SIDER databases, potential vascular drug targets such as MAP3K11, CAMK1D, PDGFD, IPO9 and CETP were not predicted to be associated with major adverse side effects (cardiovascular, neurologic, metabolic, cancer) that would result from drug inhibition. Thus, some of these genes may represent suitable potential drug targets for follow-up studies.

Impact of targeting MAP3K11 in vascular smooth muscle cells

By using the present framework, we showed that some causally associated vascular genes were central in a network and were potentially druggable. Among those genes, MAP3K11 (also known as MLK3) is positively associated with the CAD risk (OR: 1.07, 95%CI: 1.05-1.10, P_causal =8.46 x 10⁻⁹) and is a target of the experimental compound URMC-099⁴⁵. Community network analysis using random walks showed that a module including MAP3K11 (P=1.94 x 10⁻⁶) (Suppl. Figure 2) was enriched in GO for the regulation of JNK cascade (P=1.81 x 10⁻¹⁰) (Suppl. Table 17), which is involved in inflammation and cell migration⁴⁶. URMC-099 is an experimental compound that inhibits MAP3K11. We hypothesized that URMC-099 may modulate the expression of key cytokines and transcription factors involved in the development of atherosclerosis. We observed that vascular smooth muscle cells (VSMCs) treated with URMC-099 (100nM) for 6 hours had lower expression of transcripts encoding for interleukin1 beta (IL1B), C-C motif chemokine ligand 2 (CCL2 and also known as MCP1) and plasminogen activator urokinase (PLAU) (Figure 5A-C). IL1B, CCL2 and PLAU are key mediators involved in plaque activity^47–49. Early growth response 1 (EGR1) is a transcription factor known to enhance the expression of IL1B and chemokines as well as to promote the development of atherosclerosis in mice⁵⁰. In isolated VSMCs, URMC-099 reduced the transcript level encoding for EGR1 by 30% (Figure 5D). Considering the pathway enrichment of CAD gene variants in cell migration (Suppl. Table 2), we assessed the impact of URMC-099 on VSMC transmigration in a Boyden chamber. Consistent with the impact of URMC-099 on genes having a role on cell migration such as CCL2 and EGR1⁵¹, we observed a reduction of cell migration by 40% in VSMC treated with the inhibitor for 6 hours (Figure 5E). As a proof of concept, these data provide further evidence that the pharmacological inhibition of predicted causally associated genes such as MAP3K11 in VSMC impacts athero-relevant gene expression and cell phenotype.

In this work, we provide evidence that promoter-enhancer anchor loops in HCASMC explain 22% of the heritability for CAD. 3D mapped vascular smooth muscle genes were enriched in eQTL genes and in predicted causal targets. After filtering by using cross-phenotype analyses and curation of Human Phenotype Ontology and SIDER databases, we identified a set of druggable vascular genes, which are not predicted to be associated with major adverse events. Network of CAD-causally associated vascular genes was enriched in TGF-beta signaling, HDAC class I and MAPK pathways. As an illustrative case, the pharmacological inhibition of causally associated gene MAP3K11 in vascular smooth muscle cells reduced cell migration, a key process involved in the development of plaque⁵².

Among the causal candidate vascular genes identified by MR, ARRB1 (OR: 0.84, 95%CI: 0.78-0.91, P_causal=9.45 x 10⁻⁶) and MFGE8 (OR: 1.13, 95%CI: 1.09-1.17, P_causal=2.08 x 10⁻¹²) were the genes with the highest effect size on the risk of CAD. ARRB1 encodes for arrestin beta 1, which is involved in the regulation of G-protein coupled receptor (GPCR) signaling. As illustrated by the network approach, ARRB1 interacts with a large number of proteins and modulates, in a context specific manner, a myriad of signaling events related, among others, to inflammation⁵³. At the 15q26.1 locus, we found a shared signal between the vascular expression of MFGE8 and the risk of CAD (PP=0.90). Taken together, colocalization and MR analyses strongly militate for a role of MFGE8, which encodes for Milk Fat Globule EGF And Factor V/VIII Domain Containing (also known as lactadherin), on the risk of CAD. A previous study conducted in VSMCs showed that the knockdown of MFGE8 reduced the proliferation rate of cells⁵⁴. Similarly, in a mouse model of vascular injury, the silencing of MFGE8 decreased the migration of VSMCs and the formation of neointima⁵⁵. Taken together, these data are consistent with an implication of MFGE8 on the proliferation/migration of VSMC, a key process in atherogenesis.

Among the causal associations with CAD, 15 genes were identified by enhancer-promoter looping including 6 genes that were mapped only by using 3D genome data. Among those genes, several novel causal candidates, which have not been investigated in the context of atherosclerosis, were identified and include EXOSC5, B3GNT8, ARRB1, PIF1, UTP11, AGPAT4 and MAP3K11. For instance, MR data indicate that the vascular expression of AGPAT4 is negatively associated with CAD-risk. AGPAT4 encodes for a membrane enzyme that inactivates lysophosphatidic acid, a small lipid mediator with pro-atherogenic activity⁵⁶. Also, PIF1, which encodes for a helicase involved in the activity of telomerase⁵⁷, is a candidate gene for further exploration as the telomere length has been linked to the CAD-risk⁵⁸.

Network provides a holistic approach in identifying pathways and gene modules with specialized functions in chronic disorders⁵⁹. A pathway analysis of the network constructed by using the candidate causal genes showed an enrichment for TGF-beta and MAPK pathways. These data are consistent with a role of the TGF-beta pathway in atherogenesis⁶⁰. Drug targets are enriched in hub molecules⁶¹ and the present data are in line with this notion. In this regard, we found that causally associated vascular genes were acting as hub in a network and were also deemed druggable according to DGIdb. After a comprehensive assessment of potential side effects, we narrowed down the number of druggable causal candidate genes to 5 targets (MAP3K11, CAMK1D, PDGFD, IPO9 and CETP). PDGFD encodes for a platelet derived growth factor with implication in atherosclerosis⁶². CETP, which has been identified by colocalization analysis, encodes for cholesteryl ester transfer protein. CETP is involved in the metabolism of high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Genetic signal at the CETP locus suggested that inhibition of this enzyme may lower the risk of CAD⁶³. Drugs targeting CETP were evaluated in 4 different randomized clinical trials with inconsistent results, which are possibly linked to drug-related off-target effects on the blood pressure and the design of studies (reviewed in⁶⁴). In the present work, we identified by using MR that two druggable kinases (MAP3K11, CAMK1D) were positively associated with CAD. A previous analysis using MR showed that the expression of CAMK1D in blood cells was positively associated with the risk of CAD (OR: 1.05)¹⁶. These data are concordant with the present findings, which demonstrate a causal association for the vascular expression of CAMK1D on the risk of CAD (OR: 1.11). As a proof of concept, we evaluated a drug under development, which targets MAP3K11, on the expression of key genes involved in atherosclerosis and on the migration of VSMCs. In VSMCs, drug-induced inhibition of MAP3K11 reduced the expression of IL1B and CCL2, two genes causally associated with CAD^65,66. Also, the pharmacological inhibition of MAP3K11 decreased substantially the migration of VSMC, a cardinal process in the development of atheromatous plaques.

The present work has some limitations as causal inference using MR is subject to horizontal pleiotropy (i.e. gene variants that affect the outcome through an alternative pathway)⁶⁷. However, the assessment of pleiotropy with the Cochran’s Q test and also the implementation of sensitivity analyses with the median weighted analyses minimize this risk. Also, the assessment of both MR and colocalization provides robustness to the findings. As such, the combined evidence derived from colocalization and MR for a drug target (gene) increased substantially the likelihood for a drug to be licensed⁶⁸.

CAD genetic associations

Summary statistics from GWAS data of a meta-analysis including 122,733 CAD cases and 424,528 controls from the UK Biobank and CARDIoGRAMplusC4D were downloaded for analyses²⁸. GWAS meta-analysis was adjusted for age, gender and the first 30 principal components and included 7,947,838 gene variants. Cases from UK Biobank were identified by using ICD codes I21-I25 for ischemic heart disease and the corresponding OPCS-4 codes K40-K46, K49, K50 and K75 including percutaneous angioplasty. Patients reporting cardiovascular events such as myocardial infarction, coronary angioplasty and coronary artery bypass grafting were also included.

Pathway analysis of genetic association data

Genetic association data for CAD were analyzed by using DEPICT, which provides an integrative approach to assess the most likely causal genes at risk loci and to infer pathway and tissue-cell enrichments⁶⁹. Plink was used to identify independent loci based on GWAS significant SNPs (P_GWAS < 5E-8) and DEPICT was used with the prioritization of genes, gene sets and tissue-cells.

Mapping the GWAS

Genetic association data for CAD was mapped by using the Functional Mapping and Annotation of GWAS (FUMA) framework⁷⁰. Genomic risk loci were defined using a pre-calculated LD structure of the 1000G EUR reference population. SNPs in genomic loci with LD r²<0.6, P-value<5x 10^-8 and MAF≥0.01 were identified as independent significant SNPs (IndSigSNPs). IndSigSNPs independent from each other (LD r²<0.1) were identified as lead SNPs. Genomic loci closely located (<250kb based on the most right and left SNPs of each locus) were merged into one genomic locus. Gene annotation was based on Ensembl (build 85) and entrez ID yielding identification of 19,436 protein coding genes. Vascular eQTL (GTEx v8, aorta) were mapped by using IndSigSNPs to genes in cis (±500kb). SNP eQTL gene pairs were deemed significant at FDR 5%.

Analysis of HiChIP

H3K27ac-HiChIP FASTQ files (GSE101498) from HCASMC were downloaded and aligned with HiC-Pro using the default settings⁷¹. Loop call was performed with FitHiChIP⁷² at FDR < 1 x10^-6 and a resolution of 5kb. Mapping of SNP to gene promoters was performed by using bedtools with the intersect function. Gene promoters were identified as a region ± 2kb from the transcription start site (TSS) by using GENCODE version 35 in build 37. 1D H3K27ac-HiChIP track was generated using deepTools bamCoverage from the sorted BAM file generated by HiC-Pro. Hub enhancers were identified from H3K27ac-HiChIP by generating an interaction matrix of interacting pairs, which was analyzed with Cytoscape (version 3.8.2). Most connected hub enhancers were defined as those with a degree ≥ 90^th percentile.

Analysis of ATAC-seq and ChIP-seq

ATAC-seq and H3K27ac ChIP-seq data from HCASMC were downloaded (GSE124011). FASTQ files were extracted from SRA by using parallel-fastq-dump. Data were aligned on hg19 using Bowtie 2 and converted to bam files with Samtools. Duplicate reads were removed with Picard tools. Peak call was performed with MACS2 for broad peak with cutoff at 0.01. BigWig files were generated with deepTools bamCoverage and the sorted bam files.

Enrichment analysis of anchor loops with open chromatin

Anchor loops and ATAC-seq bed fileswere analyzed for enrichment of overlap by using the R package GenometriCorr⁷³. The projection test, which uses a binomial distribution was used to assess significance of calculated enrichment. Relative distance between the reference and query features were quantified by the density function correlation and illustrated in a heatmap.

Pathway analysis of anchor loops

Significant loop calls of interacting pair regions in HCASMC H3K27ac-HiChIP identified from FitHiChIP were transformed into a bed file, which integrated the interacting regions or anchor loops. Enrichment was performed by using HOMER and the KEGG pathway.

Partitioned heritability

Partitioned heritability of anchor loops in HCASMC was integrated to the full baseline model of 53 annotations in the stratified LD score regression framework³⁰. LD score was calculated for each chromosome. CAD summary statistics was converted using the munge_sumstats.py and partitioned heritability calculated using the --h2 flag.

Genetic Colocalization

Shared genetic signal between the eQTL and CAD was assessed by using the HyPrColoc package, which provides Bayesian colocalization analysis between traits⁷⁴. Genomic regions were defined as 500kb down- and 500kb up-stream of the TSS of each gene. Shared genetic signal between the expression (eQTL) and CAD was considered if the posterior probability PP>0.8. Data were visualized by using LocusCompare.

Mendelian randomization

Causal inference was evaluated with two-sample MR by selecting independent SNPs (instrumental variables) associated with the expression. SNPs were analyzed within a window of 500kb around the TSS of each gene, then only SNPs strongly associated with the expression (P<0.001 corresponds to ~F statistics>10) and independent (r²<0.1 based on the 1000G EUR reference panel) were selected as instrumental variables. MR was performed by using the Mendelian Randomisation package and a minimum of three instrumental variables was required to perform the analysis. Horizontal pleiotropy was estimated by using the Cochran’s Q test and was considered significant when P_{heterogeneity}<0.05. Genes were considered causally associated with CAD at FDR 5% using the IVW MR. Sensitivity analysis was performed with the weighted median MR.

Network analysis

Candidate causal genes were used to extract a network from the DifferentialNet dataset³⁹. Data inferred for the aorta were extracted and analyzed by using NetworkAnalyst⁷⁵. Centrality indices were downloaded from NetworkAnalyst and hub nodes (genes) were identified as those with a degree ≥ 90^th percentile. Community identification was performed by using the random walk algorithm as implemented in NetworkAnalyst. Enrichment analysis was performed in Enrichr with the BioPlanet 2019 resource.

Druggable genome

The Drug Gene Interaction Database (DGIdb) was leveraged to assess the druggability of candidate causal genes⁴⁰. Drug-gene pairs identified in DGIdb were evaluated as potential candidate by using the directional effect of the expression on the CAD risk and whether the compound was identified as an agonist or antagonist (in case of enzyme: an inhibitor).

PheWAS analysis

PheWAS analysis was performed in Gene ATLAS, which integrates 778 traits computed form 452,264 individuals in the UK Biobank⁴¹. Traits and disorders were deemed significantly associated with a SNP at FDR 5%.

Cell culture experiments

Human aorta smooth muscle cells were obtained from patients undergoing heart transplantation. All donated tissues have been obtained with an explicit written consent approved by the local ethical committee and the investigations conducted in accordance with the Helsinki Declaration. Aortic roots were cut, the tunica adventitia and tunica intima were removed, and tissues were cut into pieces and incubated 8 minutes in Trypsin (Invitrogen, Thermo Fisher Scientific, ON, Canada) at 37°C under agitation. Trypsin was then removed and tissues were resuspended in complete DMEM media (DMEM, 20% FBS with L-glutamine and sodium pyruvate). After cell growth (approximatively 1 month), DMEM media was removed and cells were cultured in smooth muscle cell basal media with growth supplements (#310-470 and #311-GS, Cell Application, CA, USA). Cells were used between passages 2 to 5. Cells were treated with 100 nM of URMC-099 (MedKoo Biosciences, NC, USA) as indicated in the result section. The expression of IL1B, CCL2, PLAU and EGR1 was evaluated by quantitative real-time PCR. RNA from cells was isolated with E.Z.N.A.® Micro RNA kit (Omega Bio-tek, VWR, QC, Canada). One μg of RNA was reverse transcribed using the Qscript cDNA supermix from Quanta (VWR, QC, Canada). qPCRs were performed with perfecta sybr supermix from Quanta on the Rotor-Gene 6000 system (Corbett Robotics Inc, CA, USA). Primers for CCL2 were obtained from Qiagen (ON, Canada) and IL1B, PLAU and EGR1 from IDT (IDT, IL, USA) (Supplementary Table 18). Transmigration assay was carried out in Boyden chamber with the QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), fluorimetric (Millipore, Burlington, USA). 10,000 cells in DMEM 0% FBS with 100 nM of URMC-099 or with the control were loaded into the insert, and DMEM 10% FBS was applied outside the insert. Cell migration was quantified after 6 hours according to manufacturer’s instructions.

Statistics

For cell analyses, continuous data were expressed as mean ± SEM. Normality was tested with the Shapiro-Wilk test. Data with normal distribution were compared with Student t-test. For data with non-normal distribution, groups were compared with the Wilcoxon-Mann-Whitney test. Statistical analyses were performed with Prism 8.0.2. Hypergeometric and binomial tests were performed by using R version 4.0. FDR was calculated by using the R package multtest with the Benjamini and Hochberg test.

In this work we found that the connectome of enhancer-promoter in HCASMC explained a significant proportion of the heritability for CAD. The mapping of genes using 3D enhancer-promoter contacts was enriched in vascular eQTL genes and causally-associated CAD genes. Among the causal candidate vascular genes, some are druggable and need further investigation to assess their role as potential pharmacologic targets for CAD.

CAD : coronary artery disease; eQTL: expression quantitative trait loci; GWAS: genetic association studies; HCASMC : human coronary artery smooth muscle cells; IVs: instrumental variables; LD: linkage disequilibrium; MR: Mendelian randomization; PheWAS: phenome-wide analysis study; RCTs: randomized clinical trials.

Ethics approval and consent to participate

All donated tissues have been obtained with an explicit written consent approved by the local ethical committee and the investigations were conducted in accordance with the Helsinki Declaration. Datasets from GEO and the GWAS summary statistics were publicly available and did not require ethical approval.

Consent for publication

Not applicable.

Availability of data and materials

All the data are available in the manuscript including in the supplementary tables and figures. GWAs summary statistics for CAD and vascular eQTL (GTEx v8, aorta) as well as FASTQ files from GEO dataset were publicly available. Accession number and URLs are provided in the manuscript methods section.

Competing interests

No competing interest to declare.

Funding

This work was supported by the Canadian Institutes of Health Research grants to P.M. (FRN148778, FRN159697) and the Quebec Heart and Lung Institute Fund. Y.B. holds a Canada Research Chair in Genomics of Heart and Lung Diseases. S.T. and B.J.A. hold a scholarship from Fonds de Recherche du Québec-Santé (FRQS).

Authors’ contributions

A.C. and P.M. conceived and designed experiments. A.C. S.M. and P.M. conducted mapping, colocalization and MR analyses. S.M. and P.M. performed statistical analyses, enrichment and network analyses. P.M. analyzed HiChIP data. Functional assays were conducted by A.C. P.M. drafted the manuscript. S.M. drafted the figures. All the authors critically reviewed the manuscript and provided intellectual inputs.

Acknowledgments

Not applicable.

Summary statistics GWAS CAD: https://data.mendeley.com/datasets/gbbsrpx6bs/1

GEO DataSets: https://www.ncbi.nlm.nih.gov/gds

GTEx v8: https://www.gtexportal.org/home/

DEPICT: https://data.broadinstitute.org/mpg/depict/

PLINK: http://zzz.bwh.harvard.edu/plink/

FUMA: https://fuma.ctglab.nl/

HiC-Pro: https://github.com/nservant/HiC-Pro

FitHiChIP: https://ay-lab.github.io/FitHiChIP/

parallel-fastq-dump: https://github.com/rvalieris/parallel-fastq-dump

Bowtie 2: http://bowtie-bio.sourceforge.net/bowtie2/index.shtml

Samtools : http://www.htslib.org/

Picard: https://broadinstitute.github.io/picard/

MACS2: https://pypi.org/project/MACS2/

deepTools: https://deeptools.readthedocs.io/en/develop/index.html

GenometriCorr: http://genometricorr.sourceforge.net/

HOMER: http://homer.ucsd.edu/homer/

Partitioned heritability: https://github.com/bulik/ldsc

HyPrColoc: https://github.com/jrs95/hyprcoloc

LocusCompare: http://locuscompare.com/

MR: https://cran.r-project.org/web/packages/MendelianRandomization/index.html

NetworkAnalyst : https://www.networkanalyst.ca/

Cytoscape : https://cytoscape.org/

DGIdb: https://www.dgidb.org/

Gene Atlas: http://geneatlas.roslin.ed.ac.uk/

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Enhancer Promoter Interactome and Mendelian Randomization Identify Network of Druggable Vascular Genes in Coronary Artery Disease

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Journal Publication

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Abstract

Figures

Introduction

Results

Tissue enrichment and pathway analyses

Heritability for CAD explained by the vascular enhancer-promoter connectome

Identification of gene promoters connected to CAD associated variants within distant enhancers

Identification of vascular eQTLs

Genetic colocalization analyses

Mendelian Randomization

Network and prioritization

Druggability of candidate causal genes

Impact of targeting MAP3K11 in vascular smooth muscle cells

Discussion

Methods

Conclusions

Abbreviations

Declarations

References

Supplementary Files

Status:

Journal Publication

Version 1